Concepts in Radiation Cell Biology

List of Contributors

Preface

Chapter 1 Ultraviolet Irradiation of DNA

I. Introduction

II. Photophysics

III. Identification of Photoproducts

IV. DNA Photoproducts and Their Properties

V. Physical and Biological Properties of Irradiated DNA

VI. Miscellaneous Topics

VII. Summary

References

Chapter 2 Methods for Photoinactivation of Viruses

I. Introduction

II. Ultraviolet Light

III. Photodynamic Action

References

Chapter 3 The Effects of Ultraviolet Radiations on Bacteria

I. Ancient History

II. The Site of UV-Induced Inactivation: Changes in Polynucleotides

III. The Fate of Pyrimidine Dimers in DNA

IV. Measurements of Radiation Sensitivity

V. Effects of Pyrimidine Dimers in D N A on Macromolecular Synthesis

VI. The Physiology of Irradiated Cells

VII. Mutation Induction—Sublethal Damage

VIII. Perspectives

References

Chapter 4 Radiation-Induced Biochemical Changes in Protozoa

I. Introduction

II. Survey

III. Changes in DNA following UV-Irradiation

IV. Biochemical Effects of Ionizing Radiation

V. Extensions

VI. Summary

References

Chapter 5 Techniques for the Analysis of Radiation-Induced Mitotic Delay in Synchronously Dividing Sea Urchin Eggs

I. Introduction

II. General Procedures for Handling Gametes and Zygotes

III. Irradiation Techniques

IV. Quantitative Analysis of Mitotic Delay

V. Special Techniques

VI. Special Advantages and Disadvantages in Comparison with Partially Synchronized Growing Cells

VII. Conclusion

References

Chapter 6 The Effects of Radiation on Mammalian Cells

I. Introduction

II. The Normal Cell Cycle

III. Radiosensitivity of the Cell Cycle

IV. Chromosome Aberrations

V. Radiation Lethality and Cell Renewal Systems

VI. Radiosensitivity of the Immune Response

VII. Radiation and Cancer

References

Chapter 7 Ionizing Radiation Effects on Higher Plants

I. Introduction

II. Actions of Radiation on Chromosomes and Mitosis

III. Actions of Radiation on Cell Growth

IV. Actions of Radiation on Senescence and Death

V. Law of Bergonie and Tribondeau

VI. Separation of "Genetic" and "Physiological" Lethality

VII. Summary

References

Chapter 8 Photodynamic Action of Laser Light on Cells

I. Introduction

II. Resume of Some Characteristics and Guidelines Relating to Photodynamic Action

III. Examples of Photosensitizing Agents

IV. Matching the Absorption Spectrum of the Photosensitizing Agent with the Wavelength of the Light Source

V. Comparison of Some Properties of Lasers and Other Light Sources

VI. Examples of Selective Destruction of Tissues, Cells, or Cell Organelles by Photodynamic Action Using Laser Light

References

Author Index

Subject Index