Concepts in Radiation Cell Biology - 1st Edition - ISBN: 9780127473505, 9780323157162

Concepts in Radiation Cell Biology

1st Edition

Editors: Gary Whitson
eBook ISBN: 9780323157162
Imprint: Academic Press
Published Date: 28th January 1972
Page Count: 296
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Concepts in Radiation Cell Biology summarizes current concepts related to the effects of radiation on cell biology, with emphasis on the underlying macromolecular basis for cellular changes in irradiated cells. It explores the effects of non-ionizing radiation, such as ultraviolet and visible light; the use of laser light in cellular studies; and the biological effects of ionizing radiation on cells. Results of ultraviolet studies implicating DNA as the main target macromolecule responsible for radiation injury, such as division delays, lethality, and delayed DNA replication, are presented. Divided into eight chapters, this volume begins with an overview of ultraviolet irradiation of DNA as well as the physical and biological properties of irradiated DNA. It then discusses methods used in the photoinactivation of viruses; the effects of ultraviolet radiation on bacteria; radiation-induced biochemical changes in protozoa; and techniques for the analysis of radiation-induced mitotic delay in synchronously dividing sea urchin eggs. The book also covers the effects of radiation on mammalian cells; the effects of ionizing radiation on higher plants; and the photodynamic effects of laser light on cells. This book is a valuable resource for cell biologists, as well as students and investigators who are seeking the necessary information for further experimentation in radiation cell biology.

Table of Contents

List of Contributors


Chapter 1 Ultraviolet Irradiation of DNA

I. Introduction

II. Photophysics

III. Identification of Photoproducts

IV. DNA Photoproducts and Their Properties

V. Physical and Biological Properties of Irradiated DNA

VI. Miscellaneous Topics

VII. Summary


Chapter 2 Methods for Photoinactivation of Viruses

I. Introduction

II. Ultraviolet Light

III. Photodynamic Action


Chapter 3 The Effects of Ultraviolet Radiations on Bacteria

I. Ancient History

II. The Site of UV-Induced Inactivation: Changes in Polynucleotides

III. The Fate of Pyrimidine Dimers in DNA

IV. Measurements of Radiation Sensitivity

V. Effects of Pyrimidine Dimers in D N A on Macromolecular Synthesis

VI. The Physiology of Irradiated Cells

VII. Mutation Induction—Sublethal Damage

VIII. Perspectives


Chapter 4 Radiation-Induced Biochemical Changes in Protozoa

I. Introduction

II. Survey

III. Changes in DNA following UV-Irradiation

IV. Biochemical Effects of Ionizing Radiation

V. Extensions

VI. Summary


Chapter 5 Techniques for the Analysis of Radiation-Induced Mitotic Delay in Synchronously Dividing Sea Urchin Eggs

I. Introduction

II. General Procedures for Handling Gametes and Zygotes

III. Irradiation Techniques

IV. Quantitative Analysis of Mitotic Delay

V. Special Techniques

VI. Special Advantages and Disadvantages in Comparison with Partially Synchronized Growing Cells

VII. Conclusion


Chapter 6 The Effects of Radiation on Mammalian Cells

I. Introduction

II. The Normal Cell Cycle

III. Radiosensitivity of the Cell Cycle

IV. Chromosome Aberrations

V. Radiation Lethality and Cell Renewal Systems

VI. Radiosensitivity of the Immune Response

VII. Radiation and Cancer


Chapter 7 Ionizing Radiation Effects on Higher Plants

I. Introduction

II. Actions of Radiation on Chromosomes and Mitosis

III. Actions of Radiation on Cell Growth

IV. Actions of Radiation on Senescence and Death

V. Law of Bergonie and Tribondeau

VI. Separation of "Genetic" and "Physiological" Lethality

VII. Summary


Chapter 8 Photodynamic Action of Laser Light on Cells

I. Introduction

II. Resume of Some Characteristics and Guidelines Relating to Photodynamic Action

III. Examples of Photosensitizing Agents

IV. Matching the Absorption Spectrum of the Photosensitizing Agent with the Wavelength of the Light Source

V. Comparison of Some Properties of Lasers and Other Light Sources

VI. Examples of Selective Destruction of Tissues, Cells, or Cell Organelles by Photodynamic Action Using Laser Light


Author Index

Subject Index


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© Academic Press 1972
Academic Press
eBook ISBN:

About the Editor

Gary Whitson

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