The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, analytical chemists, microbiologists, and cell and molecular biologists.

Table of Contents

Analytical Methods: M.C. Rose, Characterization of Human Tracheobronchial Mucin Glycoproteins. M. Fukuda, Characterization of O-Linked Saccharides from Cell Surface Glycoproteins. D.F. Smith and B.V. Torres, Lectin Affinity Chromatography of Glycolipids and Glycolipid-Derived Oligosaccharides. A. Kobata and K. Yamashita, Affinity Chromatography of Oligosaccharides on E4-Phytohemagglutinin-Agarose Column. D. Zopf, S. Ohlson, J. Dakour, W. Wang, and A. Lundblad, Analysis and Purification of Oligosaccharides by High-Performance Liquid Affinity Chromatography. R.R. Townsend, M.R. Hardy, and Y.C. Lee, Separation of Oligosaccharides Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection. M.R. Hardy, Monosaccharide Analysis of Glycoconjugates by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection. S.W. Whiteheart, A. Passaniti, J.S. Reichner, G.D. Holt, R.S. Haltiwanger, and G.W. Hart, Glycosyltransferase Probes. G.N. Misevic, Immunoblotting and Immunobinding of Acidic Polysaccharides Separated by Gel Electrophoresis. S. Pelkonen and J. Finne, Polyacrylamide Gel Electrophoresis of Capsular Polysaccharides of Bacteria. V. Kolb-Bachofen, Carbohydrate Receptor Binding Using Colloidal Gold. J. Dabrowski, Two-Dimensional Proton Magnetic Resonance Spectroscopy. R.A. Laine, Tandem Mass Spectroscopy of Oligosaccharides. Preparations: C.L.M. Stults, C.C. Sweeley, and B.A. Macher, Glycosphingolipids: Structure, Biological Source, and Properties. M. McNeil, D. Chatterjee, S.W. Hunter, and P.J. Brennan, Mycobacterial Glycolipids: Isolation, Structures,


No. of pages:
© 1989
Academic Press
Print ISBN:
Electronic ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY