Some Basic Concepts for the Choice of Methods. Techniques for the Production of Monoclonal and Polyclonal Antibodies. Lowicryl Resins. Polar and Apolar Lowicryl Resin for Immunolabeling. Comparable Yield of Immunolabel by Resin Sections and Thawed Cryosectins. Colloidal Gold Labeling for Determining Cell Surface Area. Quantitation of Colloidal Gold by Electron Microscopy. Simultaneous Demonstration of Antigens on Outer and Protoplasmic Surfaces of the Plasma Membrane by Replica Immunocytochemistry. Double Labeling of Antigenic Sites on Cell Surfaces Imaged with Backscattered Electrons. Location and Identification of Colloidal Gold Particles with an Energy Dispersive Analyzer. Use of Dinitrophenol IgG Conjugates: Immunogold Labeling of Cellular Antigens on Thin Sections of Osmicated and Epon-Embedded Specimens. Preparation and Application of Insulin-Gold Complex. Microinjection of Colloidal Gold. Combined Diaminobenzidine-Colloidal Gold Staining. Colloidal Gold Techniques and the Immunocytochemistry of Antigen-Antibody Reactions at Interfaces. Colloidal Gold Labeling for Diagnostic Pathology. Immunogold Silver Staining Using Microwave Irradiation. A Correlative Approach to Colloidal Gold Labeling with Video-Enhanced Light Microscopy, Low Voltage Scanning Electron Microscopy, and High Voltage Electron Microscopy.