Circadian Rhythms and Biological Clocks Part B - 1st Edition - ISBN: 9780128033807, 9780128033814

Circadian Rhythms and Biological Clocks Part B, Volume 552

1st Edition

Series Volume Editors: Amita Sehgal
eBook ISBN: 9780128033814
Hardcover ISBN: 9780128033807
Imprint: Academic Press
Published Date: 1st February 2015
Page Count: 414
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Table of Contents

  • Preface
  • Part I: Dissecting the Central Clock Circuit
    • Chapter One: Measuring Synchrony in the Mammalian Central Circadian Circuit
      • Abstract
      • 1 Introduction
      • 2 Monitoring SCN Rhythms with Cellular Resolution
      • 3 Isolating Data from Single Cells
      • 4 Defining a Rhythm
      • 5 Period Synchrony: Methods to Extract and Compare Periods Between Cells
      • 6 Phase Synchrony: Methods to Extract and Compare Phase Relationships Between Cells
      • 7 Perturbations Reveal Synchronization Mechanisms
      • 8 Methods Awaiting Application in Circadian Biology
      • 9 Step-by-Step Instructions for Measuring Synchrony in SCN Slice
      • Acknowledgments
    • Chapter Two: Patch-Clamp Electrophysiology in Drosophila Circadian Pacemaker Neurons
      • Abstract
      • 1 The Drosophila Circadian Network
      • 2 Circadian Control of Neuronal Activity
      • 3 Methods for Patch-Clamp Electrophysiology
      • 4 Conclusion
      • Acknowledgments
    • Chapter Three: Glial Cell Regulation of Rhythmic Behavior
      • Abstract
      • 1 Introduction
      • 2 Studies of Glial Cell Function in Circadian Behavior and Sleep
      • 3 Potential Circadian Glia–Neuron Signaling Molecules
      • 4 Molecular Genetic Strategies for Studying the Glial Regulation of Drosophila Rhythms
      • Appendix A Protocol for TRAP Profiling of Fly Glial Cells
    • Chapter Four: Neurophysiological Analysis of the Suprachiasmatic Nucleus: A Challenge at Multiple Levels
      • Abstract
      • 1 Introduction
      • 2 Part I: Clock Mechanisms at the Cellular Level
      • 3 Part II: The SCN as a Multi-Oscillator
      • 4 Part III: In Vivo Electrophysiology Recordings from the SCN in Anesthetized and Freely Moving Animals
      • 5 Conclusions
      • Acknowledgments
  • Part II: Entrainment of Central and Peripheral Clocks
    • Chapter Five: Photic Entrainment in Drosophila Assessed by Locomotor Activity Recordings
      • Abstract
      • 1 Introduction
      • 2 Different Light Regimes Used to Entrain Locomotor Activity of Fruit Flies
      • 3 Methods to Measure Locomotor Activity
      • 4 Data Analysis and System Comparison
    • Chapter Six: Photic Regulation of Clock Systems
      • Abstract
      • 1 Introduction
      • 2 The Suprachiasmatic Nuclei
      • 3 The Molecular Circadian Clock
      • 4 Peripheral Clocks
      • 5 Photoentrainment and Melanopsin
      • 6 Entrainment of the Molecular Clock
      • 7 Molecular Photoentrainment
      • 8 Studying the Effects of Light on the Circadian Clock
      • 9 Conclusions
      • Acknowledgments
    • Chapter Seven: Response of Peripheral Rhythms to the Timing of Food Intake
      • Abstract
      • 1 Introduction
      • 2 Animal Strain and Age
      • 3 Animal Room and Equipment
      • 4 Facilities to Accommodate Feeding Schedule
      • 5 Diet
      • 6 Monitoring Eating Pattern
      • 7 Physiological Readout of Eating Pattern
      • 8 Feeding Paradigms
      • 9 Mouse Tissue Collection
      • 10 Transcript, Protein, and Metabolome Expression Analysis
      • 11 Conclusion
  • Part III: Clocks and Metabolic Physiology
    • Chapter Eight: Circadian Regulation of Cellular Physiology
      • Abstract
      • 1 Introduction
      • 2 Materials
      • 3 Methods
      • 4 Notes
      • Acknowledgments
    • Chapter Nine: Analysis of the Redox Oscillations in the Circadian Clockwork
      • Abstract
      • 1 Introduction: Circadian and Redox Coupling in the Cell
      • 2 The Biochemical Properties of the Peroxiredoxin System
      • 3 Analysis of PRX Redox Oscillations
      • Acknowledgments
    • Chapter Ten: Clocks and Cardiovascular Function
      • Abstract
      • 1 Introduction
      • 2 Circadian Analysis of Physiological Parameters with Radiotelemetry
      • 3 Primary Cell Culture of Macrophages
      • 4 Circadian Variation in Thrombogenesis
      • 5 Atherosclerosis and Vascular Integrity in Models of Clock Disruption
      • 6 Clocks and Myocardial Dysfunction
      • 7 Conclusion
  • Part IV: Circadian Rhythms in Humans
    • Chapter Eleven: Measuring Circadian Clock Function in Human Cells
      • Abstract
      • 1 Introduction
      • 2 Studies of Circadian Clock Properties Using Reporters
      • 3 Ex Vivo and In Vitro Studies of Human Circadian Clocks
      • 4 Similar Technologies to Study Other Major Signaling Pathways
      • 5 Cell-Based Approaches to Study Gene Expression Variation and Human Interindividual Differences in Drug Responses
      • 6 Promise of In Vitro Gene Expression Profiling
      • 7 Specific Protocols
      • 8 Perspectives
      • Acknowledgments
    • Chapter Twelve: Human Activity and Rest In Situ
      • Abstract
      • 1 Introduction
      • 2 Probing Activity and Sleep by Questionnaires
      • 3 Measuring Activity and Sleep by Actimetry
      • 4 Concluding Remarks
    • Chapter Thirteen: Phenotyping of Neurobehavioral Vulnerability to Circadian Phase During Sleep Loss
      • Abstract
      • 1 Prevalence and Consequences of Sleep Loss
      • 2 Sleep–Wake and Circadian Regulation: Two-Process Model
      • 3 Subjective and Objective Measures for Circadian Variation in Performance
      • 4 Circadian Variation Assessment in Neurobehavioral Functions
      • 5 Sleep Deprivation and Performance
      • 6 Cumulative Effects on Performance from Chronic Sleep Restriction
      • 7 Phenotypic Individual Differences in Response to Sleep Deprivation
      • 8 The PVT: Example of a Behavioral Assay for Phenotyping Responses to Sleep Loss
      • 9 Conclusions
      • Acknowledgments
    • Chapter Fourteen: Genetics of Human Sleep Behavioral Phenotypes
      • Abstract
      • 1 Introduction
      • 2 Clinical Phenotyping
      • 3 Identification of Associated Genetic Variants
      • 4 Modeling Human Sleep Phenotypes in Rodents
      • 5 Concluding Remarks
      • Acknowledgments
    • Chapter Fifteen: Sleep and Circadian Rhythm Disruption and Recognition Memory in Schizophrenia
      • Abstract
      • 1 Introduction
      • 2 Sleep and Circadian Rhythm Disruption in Schizophrenia
      • 3 Recognition Memory Deficits in Schizophrenia
      • 4 Recognition Memory Deficits After the Direct Manipulation of Sleep and Circadian Rhythms
      • 5 Dual-Process Theory of Recognition
      • 6 Which is Impaired, Recollection or Familiarity?
      • 7 Is There an Association Between Sleep and Circadian Function and Recognition Memory in Schizophrenia?
      • 8 Summary of the Chapter and Some Unresolved Issues
      • Acknowledgments
  • Author Index
  • Subject Index


Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems.  Protocols are provided to assess clock function, entrainment of the clock to stimuli such as light and food, and output rhythms of behavior and physiology.  This volume also delves into the impact of circadian disruption on human health.  Contributions are from leaders in the field who have made major discoveries using the methods  presented here.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers research methods in biomineralization science
  • Keeping with the interdisciplinary nature of the circadian rhythm field, the volume includes diverse approaches towards the study of rhythms, from assays of biochemical reactions in unicellular organisms to monitoring of behavior in humans.


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.


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"…these volumes provide a remarkable summary of the methods, experimental systems, and findings of cutting-edge research into circadian rhythms….an essential resource for all types of scientists studying chronobiology, as well as an informative read for anyone who wishes to understand better the ticking of their biological clock." --The Quarterly Review of Biology

Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry
"The work most often consulted in the lab." --Enzymologia
"The Methods in Enzymology series represents the gold-standard." --Neuroscience

Ratings and Reviews

About the Series Volume Editors

Amita Sehgal Series Volume Editor

Dr. Sehgal is the John Herr Musser Professor of Neuroscience and Director of a Program in Chronobiology at the Perelman School of Medicine, University of Pennsylvania. She has also been an Investigator of the Howard Hughes Medical Institute since 1997. Dr. Sehgal received her Ph.D. from the Graduate School of Medical Sciences, Cornell University, working with Dr. Moses Chao, and conducted her postdoctoral work with Dr. Michael Young at Rockefeller University. A major focus in her laboratory is the cellular and molecular basis of circadian rhythms, using the fruit fly, Drosophila melanogaster as a model system. Her laboratory also developed a Drosophila model for the study of sleep, which has been adopted by laboratories worldwide, and is rapidly provding insight into the regulation and function of sleep. In 2009, Dr. Sehgal was elected to the Institute of Medicine and in 2011 to the American Academy of Arts and Sciences.

Affiliations and Expertise

John Herr Musser Professor of Neuroscience; co-Director, Penn Medicine Neuroscience Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA