Cilia: Structure and Motility

Cilia: Structure and Motility

1st Edition - November 27, 2009

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  • Editors: Stephen King, Gregory Pazour
  • Hardcover ISBN: 9780123749734
  • eBook ISBN: 9780080961064

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Description

Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.

Key Features

* Covers protocols for cilia and flagella across systems and species
* Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time
* Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

Readership

Researchers and students in cell, molecular and developmental biology.

Table of Contents

  • Cryo-Electron Microscope Tomography to Study Axonemal

    Organization

    Daniela Nicastro

    2. Electron Microscopic Imaging and Analysis of Isolated

    Dynein Particles

    Anthony J. Roberts and Stan A. Burgess

    3. Immunogold Labeling of Flagellar Components In Situ

    Stefan Geimer

    4. Scanning Electron Microscopy to Examine Cells and Organs

    Jovenal T. SanAgustin, John A. Follit, Gregory Hendricks, and Gregory J. Pazour

    5. X-ray Fiber Diffraction Studies on Flagellar Axonemes

    Kazuhiro Oiwa, Shinji Kamimura, and Hiroyuki Iwamoto

    6. Markers for Neuronal Cilia

    Jacqueline S. Domire and Kirk Mykytyn

    7. Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells

    Heymut Omran and Niki T. Loges

    8. Immunoprecipitation to Examine Protein Complexes

    Gregory J. Pazour

    9. Tandem Affinity Purification of Ciliopathy-Associated Protein

    Complexes

    Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner,

    Marius Ueffing, and Ronald Roepman

    10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products

    Miho Sakato

    11. Analysis of the Ciliary/Flagellar Beating of Chlamydomonas

    Kenneth W. Foster

    12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii

    Ritsu Kamiya

    13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain

    Karl-Ferdinand Lechtreck, Michael J. Sanderson, and George B. Witman

    14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow

    Yasushi Okada and Nobutaka Hirokawa

    15. Modification of Mouse Nodal Flow by Applying Artificial Flow

    Shigenori Nonaka

    16. Measuring Cilium-Induced Ca2þ Increases in Cultured Renal Epithelia

    Helle A. Praetorius

Product details

  • No. of pages: 344
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: November 27, 2009
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123749734
  • eBook ISBN: 9780080961064

About the Serial Volume Editors

Stephen King

Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

Affiliations and Expertise

Professor, Department of Molecular Biology and Biophysics Director, Electron Microscopy Facility, University of Connecticut Health Center

Gregory Pazour

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