Cilia: Motors and Regulation

Cilia: Motors and Regulation

1st Edition - November 23, 2009

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  • Editors: Stephen King, Gregory Pazour
  • eBook ISBN: 9780080961071
  • Hardcover ISBN: 9780123749741

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Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.

Key Features

    * Covers protocols for cilia and flagella across systems and species
    * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time
    * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures


    Researchers and students in cell, molecular and developmental biology.

    Table of Contents

    • MCB, 92

      1. Bioinformatic Approaches to Dynein Heavy Chain Classification

      Toshiki Yagi

      2. Identification and Characterization of Dynein Genes in Tetrahymena

      David E. Wilkes, Nicole Bennardo, Clarence W.C. Chan, Yu-Loung Chang,

      Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime, Julianna Erickson,

      Jonathan Hetzel, Erin E. Heyer, Mark J. Hubenschmidt, Ekaterina Kniazeva,

      Hallie Kuhn, Michelle Lum, Andrea Sand, Alicia Schep, Oksana Sergeeva,

      Natt Supab, Caroline R. Townsend, Liesl Van Ryswyk, Hadley E. Watson,

      Alice E. Wiedeman, Vidyalakshmi Rajagopalan, and David J. Asai

      3. Purification of Axonemal Dyneins and Dynein-Associated Components

      from Chlamydomonas

      Stephen M. King

      4. Purification of Dyneins from Sperm Flagella

      Kazuo Inaba and Katsutoshi Mizuno

      5. Protein Engineering Approaches to Study the Dynein Mechanism

      using a Dictyostelium Expression System

      Takahide Kon, Tomohiro Shima, and Kazuo Sutoh

      6. Biophysical Measurements on Axonemal Dyneins

      Hiroaki Kojima, Shiori Toba, Hitoshi Sakakibara, and Kazuhiro Oiwa

      7. Protein Electroporation into Chlamydomonas for Mutant Rescue

      Masahito Hayashi and Ritsu Kamiya

      8. Analysis of the Role of Nucleotides in Axonemal Dynein Function

      Chikako Shingyoji

      9. The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas

      Flagella by Axonemal Kinases and Phosphatases

      Candice A. Elam, Winfield S. Sale, and Maureen Wirschell

      10. Analysis of Redox-Sensitive Dynein Components

      Ken-ichi Wakabayashi

      11. Calcium Regulation of Ciliary Motility: Analysis of Axonemal

      Calcium-Binding Proteins

      Christen DiPetrillo and Elizabeth Smith

      12. Isolation and Analysis of Radial Spoke Proteins

      Pooja Kelekar, Mei Wei, and Pinfen Yang

      13. Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii

      David R. Mitchell and Brandon Smith

    Product details

    • No. of pages: 400
    • Language: English
    • Copyright: © Academic Press 2009
    • Published: November 23, 2009
    • Imprint: Academic Press
    • eBook ISBN: 9780080961071
    • Hardcover ISBN: 9780123749741

    About the Serial Volume Editors

    Stephen King

    Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

    Affiliations and Expertise

    Professor, Department of Molecular Biology and Biophysics Director, Electron Microscopy Facility, University of Connecticut Health Center

    Gregory Pazour

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