Cilia: Model Organisms and Intraflagellar Transport, Volume 93

1st Edition

Serial Volume Editors: Stephen King Gregory Pazour
Hardcover ISBN: 9780123813770
eBook ISBN: 9780123813787
Imprint: Academic Press
Published Date: 23rd November 2009
Page Count: 416
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Table of Contents

1. Manipulating Ciliary Protein-Encoding Genes in Tetrahymena thermophila

Drashti Dave, Dorota Wloga, and Jacek Gaertig

2. Approaches for Functional Analysis of Flagellar Proteins in African Trypanosomes

Michael Oberholzer, Miguel A. Lopez, Katherine S. Ralston, and Kent L. Hill

3. Tools for Analyzing Intraflagellar Transport in Trypanosomes

Daria Julkowska and Philippe Bastin

4. Schmidtea mediterranea: A Model System for Analysis of Motile Cilia

Panteleimon Rompolas, Ramila S. Patel-King, and Stephen M. King

5. Targeted Gene Silencing by RNA Interference in Chlamydomonas

Eun-Jeong Kim and Heriberto Cerutti

6. Analysis of Cargo Transport by IFT and GFP Imaging of IFT in Chlamydomonas

Dennis Diener

7. Genetic and Phenotypic Analysis of Flagellar Assembly Mutants

in Chlamydomonas reinhardtii

Carlo Iomini, Jacob E. Till, and Susan K. Dutcher

8. Recording and Analyzing IFT in Chlamydomonas Flagella

William Dentler, Kristyn VanderWaal, and Mary E Porter

9. Total Internal Reflection Fluorescence (TIRF) Microscopy

of Chlamydomonas Flagella

Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, and

Wallace F. Marshall

10. Purification of IFT Particle Proteins and Preparation of Recombinant

Proteins for Structural and Functional Analysis

Robert H. Behal, Ewelina Betleja, and Douglas G. Cole

11. Studying Cilia in Zebrafish

Iain Drummond

12. Analysis of IFT Kinesins in Developing Zebrafish Cone Photoreceptor

Sensory Cilia

Christine Insinna, Katherine Luby-Phelps, Brian A. Link, and Joseph C. Besharse

13. Analysis of Intraflagellar Transport in C. elegans Sensory Cilia

Limin Hao, Seyda Acar, Jame


Description

Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures.

Key Features

Includes both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time
Covers forward and reverse genetic analysis of IFT and biochemical methods to define the role of IFT components
* Methods presented cover molecular, genetic, and biochemical approaches to ciliary function in model organisms

Readership

Researchers and students in cell, molecular and developmental biology.


Details

No. of pages:
416
Language:
English
Copyright:
© Academic Press 2009
Published:
Imprint:
Academic Press
eBook ISBN:
9780123813787
Hardcover ISBN:
9780123813770

Reviews

Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures.


About the Serial Volume Editors

Stephen King Serial Volume Editor

Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

Affiliations and Expertise

University of Texas, MD Anderson Cancer Center,Michale E. Keeling Center for Comparative Medicine and Research, Bastrop, Texas, U.S.A.

Gregory Pazour Serial Volume Editor