Cilia: Model Organisms and Intraflagellar Transport

Cilia: Model Organisms and Intraflagellar Transport

1st Edition - November 23, 2009

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  • Editors: Stephen King, Gregory Pazour
  • Hardcover ISBN: 9780123813770
  • eBook ISBN: 9780123813787

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Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures.

Key Features

* Includes both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time
* Covers forward and reverse genetic analysis of IFT and biochemical methods to define the role of IFT components
* Methods presented cover molecular, genetic, and biochemical approaches to ciliary function in model organisms


Researchers and students in cell, molecular and developmental biology.

Table of Contents

  • 1. Manipulating Ciliary Protein-Encoding Genes in Tetrahymena thermophila

    Drashti Dave, Dorota Wloga, and Jacek Gaertig

    2. Approaches for Functional Analysis of Flagellar Proteins in African Trypanosomes

    Michael Oberholzer, Miguel A. Lopez, Katherine S. Ralston, and Kent L. Hill

    3. Tools for Analyzing Intraflagellar Transport in Trypanosomes

    Daria Julkowska and Philippe Bastin

    4. Schmidtea mediterranea: A Model System for Analysis of Motile Cilia

    Panteleimon Rompolas, Ramila S. Patel-King, and Stephen M. King

    5. Targeted Gene Silencing by RNA Interference in Chlamydomonas

    Eun-Jeong Kim and Heriberto Cerutti

    6. Analysis of Cargo Transport by IFT and GFP Imaging of IFT in Chlamydomonas

    Dennis Diener

    7. Genetic and Phenotypic Analysis of Flagellar Assembly Mutants

    in Chlamydomonas reinhardtii

    Carlo Iomini, Jacob E. Till, and Susan K. Dutcher

    8. Recording and Analyzing IFT in Chlamydomonas Flagella

    William Dentler, Kristyn VanderWaal, and Mary E Porter

    9. Total Internal Reflection Fluorescence (TIRF) Microscopy

    of Chlamydomonas Flagella

    Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, and

    Wallace F. Marshall

    10. Purification of IFT Particle Proteins and Preparation of Recombinant

    Proteins for Structural and Functional Analysis

    Robert H. Behal, Ewelina Betleja, and Douglas G. Cole

    11. Studying Cilia in Zebrafish

    Iain Drummond

    12. Analysis of IFT Kinesins in Developing Zebrafish Cone Photoreceptor

    Sensory Cilia

    Christine Insinna, Katherine Luby-Phelps, Brian A. Link, and Joseph C. Besharse

    13. Analysis of Intraflagellar Transport in C. elegans Sensory Cilia

    Limin Hao, Seyda Acar, James Evans, Guangshuo Ou, and Jonathan M. Scholey

    14. Functional Genomics of Intraflagellar Transport-Associated Proteins in C. elegans

    Peter N. Inglis, Oliver E. Blacque, and Michel R. Leroux

    15. Generating Conditional Mutants to Analyze Ciliary Functions:

    The Use of Cre–Lox Technology to Disrupt Cilia in Specific Organs

    Amber K. O’Connor, Robert A. Kesterson, and Bradley K. Yoder

    16. Imaging Intraflagellar Transport in Mammalian Primary Cilia

    Tatiana Y. Besschetnova, Barnali Roy, and Jagesh V. Shah

    17. Analysis of Hedgehog Signaling in Mouse Intraflagellar Transport Mutants

    Hyuk W. Ko, Aimin Liu, and Jonathan T. Eggenschwiler

Product details

  • No. of pages: 416
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: November 23, 2009
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123813770
  • eBook ISBN: 9780123813787

About the Serial Volume Editors

Stephen King

Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

Affiliations and Expertise

Professor, Department of Molecular Biology and Biophysics Director, Electron Microscopy Facility, University of Connecticut Health Center

Gregory Pazour

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