Cilia: From Mechanisms to Disease–Part A
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Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. Sections in this new release include Protocols to induce and study ciliogenesis, Flow Cytometry-Based Approach for the study of primary Cilia, Microscopic observation of human airway ciliary movement using wheat germ agglutinin, Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins, Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method, and much more. Other sections cover Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish, Visualizing Multiciliated Cells in the Zebrafish, Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas, Using Paramecium as a Model for Ciliopathies, Using organoids to study cilia, Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model, and more.
Key Features
- Offers a detailed overview of the protocols used to study cilia structure and various aspects of ciliary function
- Provides an approach to the study of some diseases related to ciliary dysfunction, also known as ciliopathies
- Written in an accessible style by renowned experts in the field
Readership
Students and entry-level scientists who are for the first time approaching the study of cilia, as well as experienced researchers.
Table of Contents
- 1. Protocols to induce and study ciliogenesis
Jose Manuel M. Bravo San Pedro
2. A Flow Cytometry-Based Approach for the study of primary Cilia
Francesca Ciccolini
3. Microscopic observation of human airway ciliary movement using wheat germ agglutinin
Yo Kishimoto
4. Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins
Koji Ikegami
5. Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method
Yumiko Saito and Yuki Kobayashi
6. High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathies
Tatsuo Miyamoto, Kosuke Hosoba, Tomoka Morita, Ying Zhang, Hiroko Kishi and Takashi Yamamoto
7. Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish
Ruxandra Bachman-Gagescu
8. Visualizing Multiciliated Cells in the Zebrafish
Rebecca Ann Wingert
9. Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas
Stephen M. King
10. Using Paramecium as a Model for Ciliopathies
Judith Van Houten
11. Using organoids to study cilia
Vincent GUEN
12. Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model
Tang K. Tang, Ting-Yu Chen and Chia-Hsiang Chang
Product details
- No. of pages: 312
- Language: English
- Copyright: © Academic Press 2023
- Published: April 1, 2023
- Imprint: Academic Press
- Hardcover ISBN: 9780443185861
About the Serial Volume Editors
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and Expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA
Jose Manuel Bravo-San Pedro
Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).
Affiliations and Expertise
Departamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain
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