Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome spread are described. The text also explains fixation and processing, including fixing of fluids and mixtures and air-drying techniques for chromosome study. The selection also discusses methods for special materials. Study of division in embryosac mother cells; study of chromosomes from thallophytes; salivary gland, lamp brush, and pachytene chromosomes; spiral structure; and secondary constriction are explained. The text also discusses microscopy, including ordinary light microscopy, high resolution autoradiography, and light microscope autoradiography. The book discusses study of plant chromosomes from tissue culture; chromosome analysis following short- and long-term cultures in animals, including man; and chromosome analysis from malignant tissues. The text takes a look at the banding patterns of chromosomes, including banding pattern techniques, C-banding, and representative schedules for comparative study of different banding patterns. The book further describes somatic cell fusion and the chemical nature of chromosomes, proteins, and enzymes. The text is a vital source of information for readers wanting to conduct research on chromosomes.
Part 1: Physical Nature of Chromosomes 1 Introduction 2 Pre-treatment and Hypotonic Treatment 3 Fixation 4 Processing 5 Staining 6 Mounting 7 Representative Schedules for Direct Observation of Chromosomes from Plants and Animals 8 Methods for Special Materials 9 Microscopy Ordinary Light Microscopy Phase and Interference Microscopy Polarization Microscopy X-ray Microscopy Fluorescence Microscopy Light Microscope Auto-radiography Electron Microscopy High Resolution Auto-radiography Micro-spectrophotometry 10 Study of Plant Chromosomes from Tissue Culture 11 Chromosome Analysis Following Short- and Long-term Cultures in Animals, Including Man 12 Chromosome Analysis from Malignant Tissues 13 Study of Banding Patterns of Chromosomes Banding pattern techniques Sister Chromatid Exchange 14 Somatic Cell Fusion 15 Effects of Physical and Chemical Agents on Chromosomes Part 2: Chemical Nature of Chromosomes 16 Introduction 17 Nucleic Acid and Its Components 18 Proteins 19 Enzymes 20 Micrurgy 21 Isolation and Extraction of Nuclei, Chromosomes and Chromosome Components 22 Molecular Hybridization in Situ Appendix 1 Culture Medium for Drosophila Appendix 2 Buffer Solutions Addendum Author Index Subject Index
- No. of pages:
- © Butterworth-Heinemann 1980
- 9th October 1980
- eBook ISBN: