Chromosome Techniques

Chromosome Techniques

Theory and Practice

3rd Edition - October 9, 1980

Write a review

  • Authors: Arun Kumar Sharma, Archana Sharma
  • eBook ISBN: 9781483100845

Purchase options

Purchase options
DRM-free (Mobi, PDF, EPub)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome spread are described. The text also explains fixation and processing, including fixing of fluids and mixtures and air-drying techniques for chromosome study. The selection also discusses methods for special materials. Study of division in embryosac mother cells; study of chromosomes from thallophytes; salivary gland, lamp brush, and pachytene chromosomes; spiral structure; and secondary constriction are explained. The text also discusses microscopy, including ordinary light microscopy, high resolution autoradiography, and light microscope autoradiography. The book discusses study of plant chromosomes from tissue culture; chromosome analysis following short- and long-term cultures in animals, including man; and chromosome analysis from malignant tissues. The text takes a look at the banding patterns of chromosomes, including banding pattern techniques, C-banding, and representative schedules for comparative study of different banding patterns. The book further describes somatic cell fusion and the chemical nature of chromosomes, proteins, and enzymes. The text is a vital source of information for readers wanting to conduct research on chromosomes.

Table of Contents

  • Part 1: Physical Nature of Chromosomes

    1 Introduction

    2 Pre-treatment and Hypotonic Treatment

    3 Fixation

    4 Processing

    5 Staining

    6 Mounting

    7 Representative Schedules for Direct Observation of Chromosomes from Plants and Animals

    8 Methods for Special Materials

    9 Microscopy

    Ordinary Light Microscopy

    Phase and Interference Microscopy

    Polarization Microscopy

    X-ray Microscopy

    Fluorescence Microscopy

    Light Microscope Auto-radiography

    Electron Microscopy

    High Resolution Auto-radiography


    10 Study of Plant Chromosomes from Tissue Culture

    11 Chromosome Analysis Following Short- and Long-term Cultures in Animals, Including Man

    12 Chromosome Analysis from Malignant Tissues

    13 Study of Banding Patterns of Chromosomes

    Banding pattern techniques

    Sister Chromatid Exchange

    14 Somatic Cell Fusion

    15 Effects of Physical and Chemical Agents on Chromosomes

    Part 2: Chemical Nature of Chromosomes

    16 Introduction

    17 Nucleic Acid and Its Components

    18 Proteins

    19 Enzymes

    20 Micrurgy

    21 Isolation and Extraction of Nuclei, Chromosomes and Chromosome Components

    22 Molecular Hybridization in Situ

    Appendix 1 Culture Medium for Drosophila

    Appendix 2 Buffer Solutions


    Author Index

    Subject Index

Product details

  • No. of pages: 724
  • Language: English
  • Copyright: © Butterworth-Heinemann 1980
  • Published: October 9, 1980
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483100845

About the Authors

Arun Kumar Sharma

Er. Arun Sharma, Assistant Research Professor, Department of Food Engineering at National Institute of Food Technology Entrepreneurship and Management (NIFTEM). Arun is an early career researcher with a research focus on nanotechnology specifically nanoparticles based nanosensors for food safety detection. He has three book chapters in press with Apple Academic Press, (USA) on Detection of Pesticides in Fruits and Vegetables: Methods and Principles. He’s currently leading three research projects at NIFTEM

Affiliations and Expertise

Assistant Research Professor, Department of Food Engineering, National Institute of Food Technology Entrepreneurship and Management (NIFTEM), Kundli, Sonipat, Haryana, India

Archana Sharma

Ratings and Reviews

Write a review

There are currently no reviews for "Chromosome Techniques"