Description

Chromatography Today provides a comprehensive coverage of various separation methods: gas, liquid, thin-layer, and supercritical fluid-chromatography, and capillary electrophoresis. Particular attention is paid to the optimization of these techniques in terms of kinetic parameters and retention mechanisms. When these facts are understood, method selection and optimization becomes a more logical process. Sample preparation methods are treated fully as they frequently represent an integral part of the total analytical method. Also described are preparative-scale separations used for isolating significant amounts of product which are generally achieved under conditions that are not identical to those used for analytical separations. The most common hyphenated methods used for sample identification are discussed from the perspective of the information they yield and the requirements of common interfaces.
The scope and level of discussion are designed to be appropriate for various user groups. This book should be suitable for use as a graduate-level student textbook in separation science, a text for professional institutes offering short courses in chromatography, and as a self-study guide for chromatographers to refresh their knowledge of the latest developments in the field. The book is extensively illustrated with over 200 figures, 110 tables and 3,300 references, largely to the contemporary literature.

Table of Contents

1. Fundamental Relationships of Chromatography Introduction. Retention. Flow in Porous Media. Band Broadening Mechanism. Peak Shape Models. Parameters Affecting Resolution. Optimization of Kinetic Column Variables. Principles of Quantitation in Column Chromatography. References. 2. The Column in Gas Chromatography Introduction. Packed Column Gas-Liquid Chromatography. Open Tubular Columns. Evaluation of the Quality of Open Tubular Columns. Retention in Gas-Liquid Chromatography. Gas-Solid Chromatography. Preparative-Scale Gas Chromatography. References. 3. Instrumental Aspects of Gas Chromatography Introduction. Pneumatic Systems. Thermal Zones. Sample Inlets. Detection Devices for Gas Chromatography. Column Connectors and Effluent Splitters. References. 4 The Column in Liquid Chromatography Introduction. Column Packing Materials. Column Preparation. Column Evaluation and Test Methods. Retention Mechanisms in Liquid Chromatography. Method Development Strategies for Liquid Chromatography. Preparative- Scale Liquid Chromatography. Capillary Electrophoretic Separation Methods. References. 5. Instrumental Aspects of High Pressure Liquid Chromatography Introduction. Requirements with Respect to Extracolumn Dispersion. Solvent Reservoirs and Solvent Degassing. Solvent Delivery Systems. Injection Devices. Detectors. References. 6. Supercritical Fluid Chromatography Introduction. Column Selection. Mobile Phase Selection. Parameters Affecting Retention. Instrumental Aspects of Supercritical Fluid Chromatography. References. 7. Thin- Layer Chromatography Introduction. Theoretica

Details

Language:
English
Copyright:
© 1991
Published:
Imprint:
Elsevier Science
Print ISBN:
9780444891617
Electronic ISBN:
9780444596192

About the authors

S.K. Poole

Affiliations and Expertise

Wayne State University, Detroit, MI, USA

Reviews

@qu:...a balanced and easy to read text. @source:Journal of High Resolution Chromatography @qu:The presentation is clear and readable... This is a worthy successor to the earlier volume and can be strongly recommended to all chromatographers. @source:Analytica Chimica Acta @qu:It is virtually impossible to do justice to a book of this size in such a short review. ...immensely valuable, and it will certainly take pride of place in my collection of chromatography reference books. @source:Chemistry in Britain @qu:This book is highly recommended for advanced undergraduate students of analytical chemistry, and is a must for practising analytical chemists. Every chapter incorporates an extensive bibliography that gives easy access to original literature. Although it is a multi-authored text it has been well compiled, so that there is no overlap of topics or ideas, and the style is very consistent. It can be emphasized that no other single current textbook on separation science gives so much for so little. @source:LC-GC International @qu:This reviewer has rarely seen a more detailed and comprehensive coverage of the entire subject of general chromatography in a single volume than in this excellent review. ...a very informative, readable and indeed enjoyable book that must be regarded as an essential addition to the bookshelves of a wide variety of users. The book will be of great value to graduate students, research workers, and, as a working reference, professional organizations running chromatographic courses. @source:Society for General Microbiology Quarterly @qu:This is perhaps the best value book I have come across on chromatography in general, with 1000 pages on gas, liquid supercritical fluid and thin-layer methods. The coverage of HPLC is naturally the most extensively (almost 300 pages), and includes a reasonable up-to-date introduction