@from:From the Preface: Chemotaxis and inflammation, as many other biological processes, can be divided into humoral and cellular components. In this simplest sense soluble activators or mediators of host or external origin interact with cells that respond to signals received and transmitted via specific membrane receptors. The biological consequences are dramatic, and the biochemical mechanisms are complex and interrelated through a series of cascades that may involve several chemical messengers of different chemical classes. Volumes 162 and 163 of Methods in Enzymology cover in vitro and in vivo methodology that has been developed for the purpose of studying the biochemistry of these active humoral factors and the biology of the cells and their receptors that respond to the various signals... The literature dealing with chemotaxis and inflammation is extensive and new techniques are constantly being developed. Therefore, some selection has been necessary to include the most commonly used and generally applicable techniques. Newer methods often involve significant modifications of established procedures, and we have tried to insure that these innovations have been included.


Biochemists, immunologists, microbiologists, pharmacologists, toxicologists, physiologists, clinical chemists, cell and molecular biologists, and biomedical scientists.

Table of Contents

Chemotaxis: A. Methods for the Study of Chemotaxis: P.C. Wilkinson and W.S. Haston, Chemotaxis: An Overview. W.S. Haston and P.C. Wilkinson, Visual Methods for Measuring Leukocyte Locomotion. P.C. Wilkinson, Micropore Filter Methods for Leukocyte Chemotaxis. R.D. Nelson and M.J. Herron, Agarose Method for Human Neutrophil Chemotaxis. J.I. Gallin, Chromium-51 Radioimmunoassay for Chemotaxis. S.H. Zigmond, Orientation Chamber in Chemotaxis. A.W. Ford-Hutchinson and J.F. Evans, Neutrophil Aggregation and Chemokinesis Assays. C.C. Daughaday, A.N. Bohrer, and I. Spilberg, Semiautomated Measurement of Neutrophil Migration with an Image Analyzer. D.A. Lauffenburger, R.T. Tranquillo, and S.H. Zigmond, Concentration Gradients of Chemotactic Factors in Chemotaxis Assays. M.D.P Boyle, M.J.P. Lawman, A.P. Gee, and M. Young, Measurement of Leukocyte Chemotaxis in Vivo. H. Gruler, Necrotaxis and Galvanotaxis. B. Methods for the Study of Chemoattractants and Biochemistry of Chemotaxis: P.C. Wilkinson, Chemotactic Factors: An Overview. N. Muthukumaraswamy and R.J. Freer, Synthesis of Chemotactic Peptides. H. Hayashi, M. Honda, Y. Mibu, S. Yamamoto, and M. Hirashima, Natural Mediators of Leukocyte Chemotaxis. J.A. Smith, Eosinophilic Chemotactic Factors of Anaphylaxis. M. Owhashi and Y. Nawa, High-Molecular-Weight Eosinophil Chemotactic Factor from Schistosoma japonicum Eggs. P.C. Wilkinson, Denatured Proteins as Chemotactic Agents: Mitogens as Lymphocyte Locomotion Activators. I. Spilberg and A.K. Bhatt, Crystal-Induc


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© 1988
Academic Press
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