Description

The understanding of chemokines, the proteins that control the migration of cells, and their receptors, is critical to the study of causes and therapies for a wide range of human diseases and infections, including certain types of cancer, inflammatory diseases, HIV, and malaria. This volume, focusing on chemokine structure and function, as well as signaling, and its companion volume (Methods in Enzymology volume 461, focusing on chemokines as potential targets for disease intervention) provide a comprehensive overview and time-tested protocols in this field, making it an essential reference for researchers in the area.

Key Features

Along with its companion volume, provides a comprehensive overview of chemokine methods, specifically as related to potential disease therapy Gathers tried, tested, and trusted methods and techniques from top players in chemokine research Provides an essential reference for researchers in the field

Readership

Researchers in cell, molecular and developmental biology; biochemists pharmacologists, and geneticists

Table of Contents

STRUCTURE AND FUNCTION 1. A Novel Chemokine-mediated In Vivo T Cell Recruitment Assay for Structure Function Studies (Andrew D. Luster and Gabriele Campanella) 2. Tyrosine sulfation of HIV-1 coreceptors and other chemokine-binding receptors (Micheal R. Farzan) 3. Lymphotactin Structural Dynamics (Brian F. Volkman) 4. Regulation of chemokines by posttranslational modifications (Paul Proost ) 5. Proteolytic Processing of Chemokines: Mass Spectrometry, Biochemistry, Neoepitope Antibody and Functional Characterization (Christopher Overall and Amanda Starr) 6. Multiple approaches to study Chemokine Receptor Homo and Heterodimerization (Mario Mellado) 7. Heterodimerization of Chemokine Receptors with Other GPCRs (Graeme Milligan and Shirley Appelbe) 8. Homo and Hetero-Oligomerization of Chemokines (Tracy Handel, Damon Hamel, Ariane Jansma) 9. Interactions of Chemokine with Glycosaminoglycans (Amanda Proudfoot) 10. Interactions of Chemokine with Glycosaminoglycans (Andreas J. Kungl) 11. Structures of Chemokine:Chemokine Binding Protein Complexes (Daved Fremont) 12. Activation Mechanisms of Chemokine Receptors (Mette Rosenkilde) 13. Modeling Small Molecule Compound Binding to Receptors (Richard Horuk, James Pease, N. Vaidehi) 14. Spectroscopic Approaches to Study Conformational Changes in GPCRs (David Farrens) 15. Plasmon Resonance Methods in GPCR Signaling (Victor Hruby, Isabel Alves, Gordon Tollin) 16. Flow Cytometric Analyses of GPCR Interactions or Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics (Larry Sklar) 17. Expression and Functional Reconstitution of CCR1 (Tracy Handel and Samantha Allen, Damon Hamel) 18. Characterization of the Interactions of Chemokin

Details

No. of pages:
350
Language:
English
Copyright:
© 2009
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080956961
Print ISBN:
9780123749079
Print ISBN:
9780323164795

About the serial-volume-editors

Tracy Handel

Professor, Skaggs School of Pharmacy and Pharmaceutical Sciences and Department of Pharmacology, School of Medicine, University of California, USA