Description

Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human diseases and infections. This volume and its companion Volume 287 Chemokines provide comprehensive experimental protocols used in this field of research.

Key Features

@introbul:Key Features @bul:* Chemokine receptors * Chemokines in disease * Signal transduction of chemokines

Readership

Biochemists, molecular biologists, immunologists, cell biologists, pharmacologists, and biomedical researchers.

Table of Contents

Chemokine Receptors: A. Richmond, S. Mueller, J.R. White, and W. Schraw, C-X-C Chemokine Receptor Desensitization Mediated through Ligand-Enhanced Receptor Phosphorylation on Serine Residues. A. Chuntharapai and K.J. Kim, Generation of Monoclonal Antibodies to Chemokine Receptors. M. Halks-Miller, J. Hesselgesser, I.J. Miko, and R. Horuk, Chemokine Receptors in Developing Human Brain. Z-X. Wang, Y-H. Cen, H-H. Guo, J-G. Du, and S.C. Peiper, Expression of Chemokine Receptors in Insect Cells Using Baculovirus Vectors. S.C. Peiper, Z-H.i Lu, T-Y. Zhang, and Z-X. Wang, Chimeric Chemokine Receptors for Analysis of Structure-Function Relationships. F.S. Monteclaro, H. Arai, and I.F. Charo, Molecular Approaches to Identifying Ligand Binding and Signaling Domains of C-C Chemokine Receptors. S. Pitchford, M. Hirst, H.G. Wada, S.D.H. Chan, V.E. Timmermans, and G.M.K. Humphries, Characterization of Functional Activity of Chemokine Receptors Using Cytosensor Microphysiometer. . P.M. Murphy, Calcium Flux Assay of Chemokine Receptor Expression in Xenopus Oocytes. J.Rucker, B.J. Doranz, A.L. Edinger, D. Long, J.F. Berson, and R.W. Doms, Cell-Cell Fusion Assay to Study Role of Chemokine Receptors in Human Immunodeficiency Virus Type 1 Entry. G.L. Bennett and R. Horuk, Iodination of Chemokines for Use in Receptor Binding Analysis. V. Fingar, H-H. Guo, Z-H. Lu, and S.C. Peiper, Expression of Chemokine Receptors by Endothelial Cells: Detection by Intravital Microscopy Using Chemokine-Coated Fluorescent Microspheres. Chemokine in Disease: V.C. Broaddus and C.A. Hebert, Neutralization of Interleukin-8 in in Vivo Models of Lung and Pleural Injury.

Details

No. of pages:
426
Language:
English
Copyright:
© 1997
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080884004
Print ISBN:
9780121821890

About the serial-volume-editor

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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY