Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human diseases and infections. This volume and its companion Volume 287 Chemokines provide comprehensive experimental protocols used in this field of research.

Key Features

@introbul:Key Features @bul:* Chemokine receptors * Chemokines in disease * Signal transduction of chemokines


Biochemists, molecular biologists, immunologists, cell biologists, pharmacologists, and biomedical researchers.

Table of Contents

Chemokine Receptors: A. Richmond, S. Mueller, J.R. White, and W. Schraw, C-X-C Chemokine Receptor Desensitization Mediated through Ligand-Enhanced Receptor Phosphorylation on Serine Residues. A. Chuntharapai and K.J. Kim, Generation of Monoclonal Antibodies to Chemokine Receptors. M. Halks-Miller, J. Hesselgesser, I.J. Miko, and R. Horuk, Chemokine Receptors in Developing Human Brain. Z-X. Wang, Y-H. Cen, H-H. Guo, J-G. Du, and S.C. Peiper, Expression of Chemokine Receptors in Insect Cells Using Baculovirus Vectors. S.C. Peiper, Z-H.i Lu, T-Y. Zhang, and Z-X. Wang, Chimeric Chemokine Receptors for Analysis of Structure-Function Relationships. F.S. Monteclaro, H. Arai, and I.F. Charo, Molecular Approaches to Identifying Ligand Binding and Signaling Domains of C-C Chemokine Receptors. S. Pitchford, M. Hirst, H.G. Wada, S.D.H. Chan, V.E. Timmermans, and G.M.K. Humphries, Characterization of Functional Activity of Chemokine Receptors Using Cytosensor Microphysiometer. . P.M. Murphy, Calcium Flux Assay of Chemokine Receptor Expression in Xenopus Oocytes. J.Rucker, B.J. Doranz, A.L. Edinger, D. Long, J.F. Berson, and R.W. Doms, Cell-Cell Fusion Assay to Study Role of Chemokine Receptors in Human Immunodeficiency Virus Type 1 Entry. G.L. Bennett and R. Horuk, Iodination of Chemokines for Use in Receptor Binding Analysis. V. Fingar, H-H. Guo, Z-H. Lu, and S.C. Peiper, Expression of Chemokine Receptors by Endothelial Cells: Detection by Intravital Microscopy Using Chemokine-Coated Fluorescent Microspheres. Chemokine in Disease: V.C. Broaddus and C.A. Hebert, Neutralization of Interleukin-8 in in Vivo Models of Lung and Pleural Injury.


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© 1997
Academic Press
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