Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems

1st Edition - May 14, 2020
This is the Latest Edition
  • Editor: David M. Chenoweth
  • eBook ISBN: 9780128201480
  • Hardcover ISBN: 9780128201459

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Description

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

      1. Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates
      2. Ophelia I. Ukaegbu, Kristen E. DeMeester, Hai Liang, Ashley R. Brown, Zachary S. Jones and Catherine Leimkuhler Grimes

      3. Chemical tools for selective activity profiling of bacterial penicillin-binding proteins
      4. Shabnam Sharifzadeh, Nathaniel W. Brown, Joshua D. Shirley, Kevin E. Bruce, Malcolm E. Winkler and Erin E. Carlson

      5. Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking
      6. Samir Gautam, Taehan Kim, Rebecca Howell and David A. Spiegel

      7. Gram-scale preparation of the antibiotic lead compound salicyl-AMS, a potent inhibitor of bacterial salicylate adenylation enzymes
      8. Nihar Kinarivala, Lisa Standke, Tezcan Guney, Cheng Ji, Naoyoshi Noguchi, Yasutomi Asano and Derek S. Tan

      9. Probe-enabled approaches for function-dependent cell sorting and characterization of microbiome subpopulations
      10. Andrea K. Steiger, Sarah J. Fansler, Christopher Whidbey and Aaron T. Wright

      11. Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity
      12. Byungchul Kim, Juliel Espinosa and Howard C. Hang

      13. Controlled release of bioactive signaling molecules using DNA-icosahedron
      14. Maulik S. Jani, Aneesh T. Veetil and Yamuna Krishnan

      15. Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation
      16. Hui Chen and Matthew C. Good

      17. Engineering reversible cell-cell interactions with chemical biology
      18. Clifford M. Csizmar and Carston R. Wagner

      19. Fast phosphine-activated control of protein function using unnatural lysine analogues
      20. Joshua S. Wesalo and Alexander Deiters

      21. Photopharmacological control of lipid function
      22. Johannes Morstein and Dirk Trauner

      23. Expanding the substrate selectivity in SNAP/CLIP-tagging of intracellular targets
      24. Miguel Macias-Contreras, Kevin N. Little and Lei Zhu

      25. Light-induced protein proximity by activation of gibberellic acid derivatives in living cells
      26. Michael J. Ziegler and Richard Wombacher

      27. Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes
      28. Daniel Z. Wu, Michael A. Lampson and David M. Chenoweth

      29. Site-specific antibody fragment conjugates for targeted imaging
      30. Robert Maloney, Zakey Yusuf Buuh, Yue Zhao and Rongsheng E. Wang

      31. Quantifying protein-protein interactions of the acyl carrier protein with solvatochromic probes

    Katherine Charov and Michael D. Burkart

Product details

  • No. of pages: 358
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: May 14, 2020
  • Imprint: Academic Press
  • eBook ISBN: 9780128201480
  • Hardcover ISBN: 9780128201459
  • About the Serial Volume Editor

    David M. Chenoweth

    David Chenoweth is at University of Pennsylvania, PA, USA

    Affiliations and Expertise

    University of Pennsylvania, PA, USA