Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems
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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems

1st Edition - May 14, 2020

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  • Editor: David M. Chenoweth
  • eBook ISBN: 9780128201480
  • Hardcover ISBN: 9780128201459

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Description

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

      1. Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates
        2.

        Ophelia I. Ukaegbu, Kristen E. DeMeester, Hai Liang, Ashley R. Brown, Zachary S. Jones and Catherine Leimkuhler Grimes

      2. Chemical tools for selective activity profiling of bacterial penicillin-binding proteins

        3.

        Shabnam Sharifzadeh, Nathaniel W. Brown, Joshua D. Shirley, Kevin E. Bruce, Malcolm E. Winkler and Erin E. Carlson

      3. Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking

        4.

        Samir Gautam, Taehan Kim, Rebecca Howell and David A. Spiegel

      4. Gram-scale preparation of the antibiotic lead compound salicyl-AMS, a potent inhibitor of bacterial salicylate adenylation enzymes

        5.

        Nihar Kinarivala, Lisa Standke, Tezcan Guney, Cheng Ji, Naoyoshi Noguchi, Yasutomi Asano and Derek S. Tan

      5. Probe-enabled approaches for function-dependent cell sorting and characterization of microbiome subpopulations

        6.

        Andrea K. Steiger, Sarah J. Fansler, Christopher Whidbey and Aaron T. Wright

      6. Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity

        7. Byungchul Kim, Juliel Espinosa and Howard C. Hang

      7. Controlled release of bioactive signaling molecules using DNA-icosahedron

        8.

        Maulik S. Jani, Aneesh T. Veetil and Yamuna Krishnan

      8. Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation

        9.

        Hui Chen and Matthew C. Good

      9. Engineering reversible cell-cell interactions with chemical biology

        10.

        Clifford M. Csizmar and Carston R. Wagner

      10. Fast phosphine-activated control of protein function using unnatural lysine analogues

        11.

        Joshua S. Wesalo and Alexander Deiters

      11. Photopharmacological control of lipid function

        12.

        Johannes Morstein and Dirk Trauner

      12. Expanding the substrate selectivity in SNAP/CLIP-tagging of intracellular targets

        13.

        Miguel Macias-Contreras, Kevin N. Little and Lei Zhu

      13. Light-induced protein proximity by activation of gibberellic acid derivatives in living cells

        14.

        Michael J. Ziegler and Richard Wombacher

      14. Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes

        15.

        Daniel Z. Wu, Michael A. Lampson and David M. Chenoweth

      15. Site-specific antibody fragment conjugates for targeted imaging

        16.

        Robert Maloney, Zakey Yusuf Buuh, Yue Zhao and Rongsheng E. Wang

      16. Quantifying protein-protein interactions of the acyl carrier protein with solvatochromic probes

    Katherine Charov and Michael D. Burkart

Product details

  • No. of pages: 358
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: May 14, 2020
  • Imprint: Academic Press
  • eBook ISBN: 9780128201480
  • Hardcover ISBN: 9780128201459

About the Serial Volume Editor

David M. Chenoweth

David Chenoweth is at University of Pennsylvania, PA, USA

Affiliations and Expertise

University of Pennsylvania, PA, USA

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