Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C - 1st Edition - ISBN: 9780128191286

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633

1st Edition

Serial Volume Editors: Arun Shukla
Hardcover ISBN: 9780128191286
Imprint: Academic Press
Published Date: 15th February 2020
Page Count: 412
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Table of Contents

    1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein
    2. Elke Oberbichler, Maria Wiesauer, Eva Schlögl, Jessica Stangl, Felix Faschinger, Günther Knör, Hermann J. Gruber and Vesa P. Hytönen

    3. (Strept)avidin as a template for ligands other than biotin: An overview
    4. Vesa P. Hytönen

    5. Engineered siderophores
    6. Sina Rütschlin and Thomas Böttcher

    7. Competitive profiling for enzyme inhibitors using chemical probes
    8. Michaela Prothiwa and Thomas Böttcher

    9. The NMR tube bioreactor
    10. Alexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos

    11. A chemically-controlled system for activating RAS GTPases
    12. Emily M. Dieter and Dustin J. Maly

    13. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9
    14. Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler

    15. Synthetic receptors to understand and control cellular functions
    16. Hung-Ju Chang and Jerome Bonnet

    17. A suite of bioassays to evaluate CREB inhibitors
    18. Bingbing X. Li and Xiangshu Xiao

    19. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe
    20. Xiangshu Xiao and Bingbing X. Li

    21. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteins
    22. Marcus J. C. Long, Daniel A. Urul and Yimon Aye

    23. Building artificial genetic circuits to understand protein function
    24. Louis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham

    25. Methods for studying human sirtuins with activity-based chemical probes
    26. Song Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen

    27. Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors
    28. Ashish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla

    29. Reversible biotinylation of purified proteins for measuring protein-protein interactions

Hemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla


Description

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more.

Key Features

  • Contains the authority of authors who are leaders in their field
  • Provides a comprehensive source on new methods and research in enzymology

Readership

Experts in the field who want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques


Details

No. of pages:
412
Language:
English
Copyright:
© Academic Press 2020
Published:
15th February 2020
Imprint:
Academic Press
Hardcover ISBN:
9780128191286

Ratings and Reviews


About the Serial Volume Editors

Arun Shukla

Arun Shukla Serial Volume Editor

Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.

Affiliations and Expertise

Indian Institute of Technology, Kanpur, India