Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C - 1st Edition - ISBN: 9780128191286

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633

1st Edition

Serial Volume Editors: Arun Shukla
Hardcover ISBN: 9780128191286
Imprint: Academic Press
Published Date: 15th February 2020
Page Count: 412
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Table of Contents

1. Next generation probes for molecular imaging in cells
Jefferson Chan
2. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein
Vesa P. Hytönen
3. Converting avidin to bind ligands other than biotin, especially steroids
Vesa P. Hytönen
4. Designing N-glycan Isomer Library
Peng George Wang
5. Chemoenzymatic Labeling Strategy
Peng George Wang
6. Engineered Siderophores
Thomas Böttcher
7. Small molecules to inhibit bacterial population behavior
Thomas Böttcher
8. NMR tube bioreactor
Andreas G Tzakos
9. Small molecule controlled RAS activation system
Dustin J. Maly
10. Small molecule regulated Cas9
Dustin J. Maly
11. Design and application synthetic receptors
Jerome Bonnet
12. GLP-1R localization and signalling interrogated by a chemical biology approach using LUXendins
Johannes Broichhagen
13. Design and application of NMR Tube Bioreactor
Andreas G Tzakos
14. Characterization of Chemical Inhibitors and Inducers or Autophagy: Research and Therapeutic Considerations
Rodrigo Franco
15. Designer tools for CREB inhibition
Xiangshu Xiao
16. Identification of Lamins as the Molecular Targets of LBL1 using a clickable photoaffinity probe
Xiangshu Xiao
17. REX technologies for profiling and decoding the electrophile signalling axes mediated by Rosetta stone proteins
Yimon Aye
18. Engineering chimeric receptors for ca2+ signals
Jordan Meier
19. Building artificial genetic circuits to understand protein function
Oliver Rackham
20. Activity-based chemical probes for the human sirtuins
Yana Cen
21. Discovery and application of cyclotides
David J. Craik
22. Bimane labeling of β-arrestins to measure their interaction with GPCRs
Arun K. Shukla
23. Reversible biotinylation of proteins for investigating their interaction with partners
Arun K. Shukla


This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Key Features

  • Contains the authority of authors who are leaders in their field
  • Provides a comprehensive source on new methods and research in enzymology


Experts in the field who want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques


No. of pages:
© Academic Press 2020
15th February 2020
Academic Press
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editors

Arun Shukla

Arun Shukla Serial Volume Editor

Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.

Affiliations and Expertise

Indian Institute of Technology, Kanpur, India