Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part B

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part B

1st Edition - May 30, 2019

Write a review

  • Editor: Arun Shukla
  • eBook ISBN: 9780128181201
  • Hardcover ISBN: 9780128181195

Purchase options

Purchase options
DRM-free (Mobi, PDF, EPub)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics


Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech professionals, natural product chemists, drug discovery researchers

Table of Contents

  • 1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systems
    Brianna E.K. Jayanthi, Wenting Zhao and Laura Segatori
    2. Design and synthesis of fluorescent activity probes for protein phosphatases
    Garrett R. Casey, Jon R. Beck and Cliff I. Stains
    3. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells
    Travis J. Nelson, Jia Zhao and Cliff I. Stains
    4. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation
    Justin Lawrie, Wei Niu and Jiantao Guo
    5. Synthesis and screening of bead-displayed combinatorial libraries
    Todd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek
    6. Visualizing the brain's astrocytes
    Alyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin
    7. Modular activatable bioorthogonal reagents
    Pratik Kumar and Scott T. Laughlin
    8. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptors
    Sam Lear, Zaid Amso and Weijun Shen
    9. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes
    Lyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi
    10. Selection and optimization of enzyme reporters for chemical cytometry
    Angela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton
    11. Synthetic cell-like membrane interfaces for probing dynamic protein–lipid interactions
    Spencer T. Glantz, Erin E. Berlew and Brian Y. Chow
    12. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity
    Wan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu
    13. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blotting
    Narek Darabedian and Matthew R. Pratt
    14. Optogenetic perturbation of the biochemical pathways that control cell behavior
    Lauren L. Haar, David S. Lawrence and Robert M. Hughes
    15. pH-shift stress on antibodies
    Hiroshi Imamura and Shinya Honda
    16. Small molecule target identification using photo-affinity chromatography
    Seung-Yong Seo and Timothy W. Corson
    17. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetases
    Andrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai
    18. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cells
    Ryou Kubota, Shigeki Kiyonaka and Itaru Hamachi
    19. Bioorthogonal oncometabolite ligation
    Chloe A. Briney, Susana Najera and Jordan L. Meier

Product details

  • No. of pages: 466
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: May 30, 2019
  • Imprint: Academic Press
  • eBook ISBN: 9780128181201
  • Hardcover ISBN: 9780128181195

About the Serial Volume Editor

Arun Shukla

Arun Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

Affiliations and Expertise

Indian Institute of Technology, Kanpur, India

Ratings and Reviews

Write a review

There are currently no reviews for "Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part B"