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1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systems
Brianna E.K. Jayanthi, Wenting Zhao and Laura Segatori
2. Design and synthesis of fluorescent activity probes for protein phosphatases
Garrett R. Casey, Jon R. Beck and Cliff I. Stains
3. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells
Travis J. Nelson, Jia Zhao and Cliff I. Stains
4. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation
Justin Lawrie, Wei Niu and Jiantao Guo
5. Synthesis and screening of bead-displayed combinatorial libraries
Todd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek
6. Visualizing the brain's astrocytes
Alyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin
7. Modular activatable bioorthogonal reagents
Pratik Kumar and Scott T. Laughlin
8. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptors
Sam Lear, Zaid Amso and Weijun Shen
9. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes
Lyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi
10. Selection and optimization of enzyme reporters for chemical cytometry
Angela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton
11. Synthetic cell-like membrane interfaces for probing dynamic protein–lipid interactions
Spencer T. Glantz, Erin E. Berlew and Brian Y. Chow
12. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity
Wan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu
13. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blotting
Narek Darabedian and Matthew R. Pratt
14. Optogenetic perturbation of the biochemical pathways that control cell behavior
Lauren L. Haar, David S. Lawrence and Robert M. Hughes
15. pH-shift stress on antibodies
Hiroshi Imamura and Shinya Honda
16. Small molecule target identification using photo-affinity chromatography
Seung-Yong Seo and Timothy W. Corson
17. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetases
Andrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai
18. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cells
Ryou Kubota, Shigeki Kiyonaka and Itaru Hamachi
19. Bioorthogonal oncometabolite ligation
Chloe A. Briney, Susana Najera and Jordan L. Meier
Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
- Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics
Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech professionals, natural product chemists, drug discovery researchers
- No. of pages:
- © Academic Press 2019
- 30th May 2019
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY
"The work most often consulted in the lab." --ENZYMOLOGIA
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE
Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).
Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.
Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.
Indian Institute of Technology, Kanpur, India
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