Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A - 1st Edition - ISBN: 9780128181171

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621

1st Edition

Serial Volume Editors: Arun Shukla
Hardcover ISBN: 9780128181171
Imprint: Academic Press
Published Date: 3rd June 2019
Page Count: 382
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Table of Contents

    1. Targeted m6A reader proteins to study the epitranscriptome
    2. Simone Rauch and Bryan C. Dickinson

    3. Synthetic gene expression circuits regulating sexual reproduction
    4. Nobuo Fukuda and Shinya Honda

    5. Nucleotide resolution sequencing of N4-acetylcytidine in RNA
    6. Justin M. Thomas, Keri M. Bryson and Jordan L. Meier

    7. A guide for drug inducible genome editing with HIT systems
    8. Chen Zhao, Shixian Wei and Yu Wang

    9. A guide for drug inducible transcriptional activation with HIT systems
    10. Chen Zhao, Shixian Wei and Yu Wang

    11. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination
    12. Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore

    13. Salt-sensitive intein for large-scale polypeptide production
    14. Yi-Zong Lee and Shih-Che Sue

    15. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls
    16. M. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg

    17. Design, cloning and characterization of transcription factor-based inducible gene expression systems
    18. Erik K.R. Hanko, Nigel P. Minton and Naglis Malys

    19. Design, construction, and validation of optogenetic proteins
    20. Colin P. O'Banion, Anwesha Goswami and David S. Lawrence

    21. Overcoming component limitations in synthetic biology through transposon-mediated protein engineering
    22. Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg

    23. Chemical biology of glycoproteins: From chemical synthesis to biological impact
    24. Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan

    25. In bulla functional channel expression systems that mimic bacterial synthetic membranes
    26. Masayuki Iwamoto and Shigetoshi Oiki

    27. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems
    28. John E. Gadbery and Nicole S. Sampson

    29. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases
    30. David Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin

    31. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy
    32. Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia

    33. Improved sensitivity and resolution of in-cell NMR spectra
    34. David S. Burz, Leonard Breindel and Alexander Shekhtman

    35. High-throughput methods in aptamer discovery and analysis
    36. Kyle H. Cole and Andrej Lupták

    37. Drop-in-well chamber for droplet interface bilayer with built-in electrodes

Kazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki


Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this volume covering Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers, the Design and synthesis of fluorescent activity probes for protein phosphatases, Stains, Utilizing split-nanoLuc fragments as luminescent probes for protein solubility in living cells, SH2-domain based sensor for intracellular recognition of sulfo-tyrosine, DNA-encoded immunoglobulins for detection of parasites, An engineered TEV protease – calmodulin fusion based sensor for neuronal calcium recording, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics


Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech professionals, natural product chemists, drug discovery researchers


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© Academic Press 2019
Academic Press
Hardcover ISBN:


Praise for the Series:
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"The work most often consulted in the lab." --ENZYMOLOGIA
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

Ratings and Reviews

About the Serial Volume Editors

Arun Shukla

Arun Shukla Serial Volume Editor

Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.

Affiliations and Expertise

Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India