Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621

1. Targeted m6A reader proteins to study the epitranscriptome

Simone Rauch and Bryan C. Dickinson

2. Synthetic gene expression circuits regulating sexual reproduction

Nobuo Fukuda and Shinya Honda

3. Nucleotide resolution sequencing of N4-acetylcytidine in RNA

Justin M. Thomas, Keri M. Bryson and Jordan L. Meier

4. A guide for drug inducible genome editing with HIT systems

Chen Zhao, Shixian Wei and Yu Wang

5. A guide for drug inducible transcriptional activation with HIT systems

Chen Zhao, Shixian Wei and Yu Wang

6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination

Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore

7. Salt-sensitive intein for large-scale polypeptide production

Yi-Zong Lee and Shih-Che Sue

8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls

M. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg

9. Design, cloning and characterization of transcription factor-based inducible gene expression systems

Erik K.R. Hanko, Nigel P. Minton and Naglis Malys

10. Design, construction, and validation of optogenetic proteins

Colin P. O'Banion, Anwesha Goswami and David S. Lawrence

11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineering

Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg

12. Chemical biology of glycoproteins: From chemical synthesis to biological impact

Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan

13. In bulla functional channel expression systems that mimic bacterial synthetic membranes

Masayuki Iwamoto and Shigetoshi Oiki

14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems

John E. Gadbery and Nicole S. Sampson

15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases

David Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin

16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy

Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia

17. Improved sensitivity and resolution of in-cell NMR spectra

David S. Burz, Leonard Breindel and Alexander Shekhtman

18. High-throughput methods in aptamer discovery and analysis

Kyle H. Cole and Andrej Lupták

19. Drop-in-well chamber for droplet interface bilayer with built-in electrodes

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this volume covering Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers, the Design and synthesis of fluorescent activity probes for protein phosphatases, Stains, Utilizing split-nanoLuc fragments as luminescent probes for protein solubility in living cells, SH2-domain based sensor for intracellular recognition of sulfo-tyrosine, DNA-encoded immunoglobulins for detection of parasites, An engineered TEV protease – calmodulin fusion based sensor for neuronal calcium recording, and much more.

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
• Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics

Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech professionals, natural product chemists, drug discovery researchers