Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Labelling - 1st Edition - ISBN: 9780128181195

Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Labelling, Volume 622

1st Edition

Serial Volume Editors: Arun Shukla
Hardcover ISBN: 9780128181195
Imprint: Academic Press
Published Date: 15th June 2019
Page Count: 412
Sales tax will be calculated at check-out Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

1. Design of optogenetic proteins
David S. Lawrence
2. Application of optogenetic proteins
David S. Lawrence
3. Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy
Shinya Honda
4. Bimane labeling of β-arrestins to measure their interaction with GPCRs
Arun K. Shukla
5. Reversible biotinylation of proteins for investigating their interaction with partners
Arun K. Shukla
6. Chemical biology approaches to study RNA cytidine acetylation
Jordan Meier
7.. Salt sensitive intein in robotic production of peptides
Shih-Che Sue
8. Isotope-coded Mass Spec Tags to study interfacial protein-membrane interactions
Nicole S. Sampson
9. Direct Cloning by Transformation-Associated Recombination and Heterologous Expression of Biosynthetic Gene Cluster
Bradley Moore
10. Allosteric protein switches: from rational design to real applications
John Karanicolas
11. Recombinant expression of protein kinases and their use for synthetic biology
Stefan T. Arold
12. Small molecule target identification using photo-affinity chromatography
Timothy W. Corson
13. Studying protein interactions by in-cell NMR
Alexander Shekhtman
14. Investigating the role of glycosylation in protein structure, function, and stability
Zhongping Tan
15. Drug inducible genome editing
Yu Wang
16. Drug inducible genome activation
Yu Wang
17. Computation biology towards directed biosynthesis of natural peptide products
Shiou-Chuan (Sheryl) Tsai
18. Emerging protein engineering approaches for imaging applications
Itaru Hamachi
19. Engineering structure and function of glycosyltransferases
Marcelo E. Guerin
20. Using Cas-Targeted m6A reader proteins to study the effects of reader proteins on single RNAs
Bryan C. Dickinson
21. Synthetic genetic circuits for regulation of sexual reproduction
Shinya Honda


Description

Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Labelling, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics

Readership

Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech professionals, natural product chemists, drug discovery researchers


Details

No. of pages:
412
Language:
English
Copyright:
© Academic Press 2019
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128181195

Reviews

Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY
"The work most often consulted in the lab." --ENZYMOLOGIA
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

Ratings and Reviews


About the Serial Volume Editors

Arun Shukla

Arun Shukla Serial Volume Editor

Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.

Affiliations and Expertise

Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India