Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B - 1st Edition - ISBN: 9780128181195

Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622

1st Edition

Serial Volume Editors: Arun Shukla
Hardcover ISBN: 9780128181195
Imprint: Academic Press
Published Date: 17th June 2019
Page Count: 466
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Table of Contents

    1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systems
    2. Brianna E.K. Jayanthi, Wenting Zhao and Laura Segatori

    3. Design and synthesis of fluorescent activity probes for protein phosphatases
    4. Garrett R. Casey, Jon R. Beck and Cliff I. Stains

    5. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells
    6. Travis J. Nelson, Jia Zhao and Cliff I. Stains

    7. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation
    8. Justin Lawrie, Wei Niu and Jiantao Guo

    9. Synthesis and screening of bead-displayed combinatorial libraries
    10. Todd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek

    11. Visualizing the brain's astrocytes
    12. Alyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin

    13. Modular activatable bioorthogonal reagents
    14. Pratik Kumar and Scott T. Laughlin

    15. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptors
    16. Sam Lear, Zaid Amso and Weijun Shen

    17. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes
    18. Lyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi

    19. Selection and optimization of enzyme reporters for chemical cytometry
    20. Angela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton

    21. Synthetic cell-like membrane interfaces for probing dynamic protein–lipid interactions
    22. Spencer T. Glantz, Erin E. Berlew and Brian Y. Chow

    23. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity
    24. Wan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu

    25. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blotting
    26. Narek Darabedian and Matthew R. Pratt

    27. Optogenetic perturbation of the biochemical pathways that control cell behavior
    28. Lauren L. Haar, David S. Lawrence and Robert M. Hughes

    29. pH-shift stress on antibodies
    30. Hiroshi Imamura and Shinya Honda

    31. Small molecule target identification using photo-affinity chromatography
    32. Seung-Yong Seo and Timothy W. Corson

    33. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetases
    34. Andrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai

    35. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cells
    36. Ryou Kubota, Shigeki Kiyonaka and Itaru Hamachi

    37. Bioorthogonal oncometabolite ligation

Chloe A. Briney, Susana Najera and Jordan L. Meier


Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics


Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech professionals, natural product chemists, drug discovery researchers


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© Academic Press 2019
Academic Press
Hardcover ISBN:


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"The work most often consulted in the lab." --ENZYMOLOGIA
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

Ratings and Reviews

About the Serial Volume Editors

Arun Shukla

Arun Shukla Serial Volume Editor

Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.

Affiliations and Expertise

Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India