Changes in Eukaryotic Gene Expression in Response to Environmental Stress

Changes in Eukaryotic Gene Expression in Response to Environmental Stress

5th Edition - February 27, 1985

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  • Editor: Burr Atkinson
  • eBook ISBN: 9780323162227

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Changes in Eukaryotic Gene Expression in Response to Environmental Stress focuses on various aspects of eukaryotic cell's response to heat stress (shock) and other stress stimuli. This book is organized into two major sections, encompassing 17 chapters that reflect the emphasis on research utilizing Drosophila, a variety of animal systems, and plants. This book first provides a brief introduction to the organization, sequences, and induction of heat shock proteins and related genes. It then describes the control of transcription during heat shock from the standpoint of molecular biology and evolutionary variations of the mechanisms in organisms with diverse metabolic needs. It goes on to discuss the issue of coordinate and noncoordinate responses of heat shock genes. It presents a model for post-transcriptional regulation on certain aspects of coordinate and noncoordinate regulations. Chapters 6-12 discuss heat shock proteins and genes and the effects of stress on gene expression of sea urchin, avian, and mammalian cells. The second part of the book focuses on the physiological role of heat shock proteins and genes in plants and fungi. It includes a discussion on experimental problems encountered during studies of the mechanisms of inhibition of photosynthesis by unfavorable environmental conditions. The changes in transcription and translation of specific mRNAs in the developing embryo during heat shock at various temperatures are described. The concluding chapters deal with heat shock response in plants, particularly the response in soybeans and maize, covering both physiological and molecular analyses. Research scientists, clinicians, and agriculturists will greatly benefit from the information presented in this book.

Table of Contents

  • Contributors


    I. Animals

    1 Organization, Sequences, and Induction of Heat Shock Genes

    I. Introduction

    II. Organization and Sequences

    III. Induction


    2 Mechanism of Transcriptional Control during Heat Shock

    I. Introduction

    II. Phenomenology of Transcriptional Control

    III. Identification of Regulatory Mechanism

    IV. Transcriptional Induction in Vitro

    V. Nature of Inducer

    VI. Mechanism of Transcriptional Control


    3 Mechanism of Translational Control in Heat-Shocked Drosophila Cells

    I. Introduction

    II. Studies of Translational Control in Intact Drosophila Cells

    III. Studies Using Cell-Free Translation Systems

    IV. Studies Defining the Steps at Which Protein Synthesis Is Altered in Heat-Shocked Cells

    V. Summary


    4 Coordinate and Noncoordinate Gene Expression during Heat Shock: A Model for Regulation

    I. Introduction

    II. Basic Features of Heat Shock Response

    III. Major Control Points of Heat Shock Gene Regulation

    IV. Distinction between Coordinate and Noncoordinate Aspects of Regulation

    V. Other Recent Findings Relevant to Regulation

    VI. A Model for Regulation


    5 Intracellular Localization and Possible Functions of Heat Shock Proteins

    I. Introduction

    II. Biochemical Studies on Heat Shock Protein Localization

    III. Immunocytochemical Localization of Heat Shock Proteins

    IV. Putative Function of Heat Shock Proteins

    V. Summary


    B. Other Animals

    6 Heat Shock Proteins in Sea Urchin Development

    I. Introduction

    II. Heat Treatment of Embryos at Gastrula Stage

    III. Heat Treatment of Embryos at Different Developmental Stages

    IV. Fate of the Heat Shock Proteins after Reversal to Normal Protein Synthesis

    V. Heat Shock Protein Synthesis in Dissociated Cells

    VI. Tissue Specificity in the Production of Heat Shock Proteins

    VII. Intracellular Location of Heat Shock Proteins

    VIII. Bulk Protein Synthesis Inhibition after Heating

    IX. Dependence of Heat Shock Protein Synthesis on Synthesis of Corresponding mRNA's


    7 Heat Shock Gene Expression during Early Animal Development

    I. Introduction

    II. Sea Urchin

    III. Xenopus laevis

    IV. Mouse and Rabbit Preimplantation Embryos

    V. Conclusions


    8 Effects of Stress on the Gene Expression of Amphibian, Avian, and Mammalian Blood Cells

    I. Introduction

    II. Elaboration of a Thermal Stress Response in Cultured Red Blood Cells from Normal (Nonanemic) and Phenylhydrazine-Treated (Anemic) Adult Quail

    III. Characterization of the Heat Shock and Stress Proteins Induced in Cultured Red Blood Cells from Anemic Adult Quail

    IV. Comparison of Quail Red Blood Cell Heat Shock Proteins Induced in Culture with Those Induced in Situ

    V. Characterization of the Response of Red Blood Cells from Anemic Quail to Heat Shock and Chemical Stress

    VI. Conclusion


    9 Stress Response in Avian Cells

    I. Introduction

    II. Stressors of Avian Cells

    III. Induction and Deinduction

    IV. Major Avian Stress Proteins

    V. Conclusions


    10 Stress Responses in Avian and Mammalian Cells

    I. Introduction

    II. Purification of Three Major Rat Stress Proteins

    III. Extracellular Appearance of Rat Stress Proteins

    IV. Stimulation of Stress mRNA Synthesis in Chicken Embryo Cells Exposed to Canavanine or Heat

    V. Inhibitors of the Stress Response

    VI. Summary


    11 Effect of Hyperthermia and LSD on Gene Expression in the Mammalian Brain and Other


    I. Introduction

    II. Inhibitory Effect of LSD on Brain Protein Synthesis

    III. Effect of Hyperthermia on Brain Protein Synthesis

    IV. Induction of Heat Shock Protein in Intact Mammalian Organs

    V. Developmental Changes in the Inducibility of Heat Shock Proteins

    VI. Heat Shock Protein in Specific Cellular Systems in Brain

    VII. Induction of mRNA Coding for Heat Shock Protein

    VIII. Conclusions


    12 Thermotolerance in Mammalian Cells: A Possible Role for Heat Shock Proteins

    I. Introduction

    II. Thermotolerance in Mammalian Systems

    III. Correlation between Synthesis of Heat Shock Proteins and Development of Thermotolerance

    IV. Kinetics of Heat Shock Protein Synthesis during Development of Thermotolerance: Effects of Temperature and Duration of Initial Heat Treatment

    V. Relationship between Levels of Heat Shock Proteins and Cellular Survival during Decay of Thermotolerance

    VI. Induction of Thermotolerance and Enhanced Synthesis of Heat Shock Proteins by Agents Other Than Heat

    VII. Effect of Amino Acid Analogs on Thermal Sensitivity and Development of Thermotolerance

    VIII. Stable Heat-Resistant Variants of Chinese Hamster Fibroblasts

    IX. Heat-Induced Protection of Mice against Thermal Death

    X. Induction of Thermal Tolerance and Enhanced Synthesis of Heat Shock Proteins in Murine Tumors

    XI. Clinical Relevance


    II. Plants and Fungi

    13 Heat Shock Genes of Dictyostelium

    I. Introduction

    II. Physiological Role of Heat Shock Proteins

    III. Induction of Heat Shock Genes

    IV. Control of Transcription

    V. The Heat Shock Protein 70 Gene of Dictyostelium

    VI. A Heat Shock-Induced Message Is Encoded by a Transposable Element

    VII. Heat Shock Proteins


    14 Plant Productivity, Photosynthesis, and Environmental Stress

    I. Introduction

    II. Research Strategy

    III. Conclusions


    15 Responses to Environmental Heat Stress in the Plant Embryo

    I. Introduction

    II. Storage Protein Synthesis Continues at Higher Rates at Heat Shock Temperatures in the Developing Soybean Embryo

    III. Synthesis of Specific Messenger RNA's during Heat Shock in Developing Soybean Embryos

    IV. Conclusions


    16 Physiological and Molecular Analyses of the Heat Shock Response in Plants

    I. Introduction

    II. Results

    III. Discussion and Summary


    17 Maize Genome Response to Thermal Shifts

    I. Introduction

    II. Characterization of the Heat Shock Response in Maize (cv. Oh43) Seedlings

    III. Influence of Growing Temperature and Thermal Shifts on Gene Expression in Maize (cv. Oh43) Seedlings

    IV. Impact of Genotype on Polypeptide Synthesis in Maize Seedlings

    V. Summary



Product details

  • No. of pages: 400
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: February 27, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9780323162227

About the Editor

Burr Atkinson

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