Centrosomes and Spindle Pole Bodies - 1st Edition - ISBN: 9780125441704, 9780080496627

Centrosomes and Spindle Pole Bodies, Volume 67

1st Edition

Editors: Robert Palazzo Trisha Davis
Hardcover ISBN: 9780125441704
eBook ISBN: 9780080496627
Imprint: Academic Press
Published Date: 27th August 2001
Page Count: 375
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Table of Contents

Contributors. Preface. Purification and Reconstitution of Drosophilia g- - Tubulin Complexes. Using Rapid Freeze and Freeze-Substitution for the Preparation of Yeast Cells for Electron Microscopy and Three-Dimensional Analysis. Digital Fluorescence Microscopy of Cell Cytoplasts with and without the Centrosome. Methods for the Study of Pericentrin in Centrosome Assembly and Function. Molecular Dissection of Yeast Spindle Pole Bodies by Two Hybrid in Vitro Binding, and Co-purification. Genetic Analysis of Yeast Spindle Pole Bodies. Methods for the Study of Centrosomes in Drosophilia during Embryogenesis. Methods for Identification of Centrosome-Associated Proteins. Reconstitution of Centrosome Microtubule Nucleation in Drosophilia. Reconstitution of Centrosome Microtubule Nucleation in Spisula.

In Vitro Approaches for the Study of Microtubule Nucleation at the Fission Yeast Spindle Pole Body. Gamma Tubulin and Microtubule Nucleation in Mammalian Cells. Gamma Tubulin in Plant Cells. Centrosomes and Parthenogenesis.

In Vitro Approaches for the Study of Molecular Motors in Aster Formation. Methods for the Study of Centrosome-Independent Spindle Assembly in Xenopus Extracts. Methods for the Study of Centrosome Reproduction in Mammalian Cells. Centrosome Reproduction in Xenopus Lysates. Centrosome Reproduction in Vitro: Mammalian Centrosomes in Xenopus Lysates. Identification of Centrosome Kinases. Methods for the Analysis of Centrosome Reproduction in Cancer Cells. Isolation of Centrosomes from Dictyostelium. Index. Volume in Series.


Description

Contributors. Preface. Purification and Reconstitution of Drosophilia g- - Tubulin Complexes. Using Rapid Freeze and Freeze-Substitution for the Preparation of Yeast Cells for Electron Microscopy and Three-Dimensional Analysis. Digital Fluorescence Microscopy of Cell Cytoplasts with and without the Centrosome. Methods for the Study of Pericentrin in Centrosome Assembly and Function. Molecular Dissection of Yeast Spindle Pole Bodies by Two Hybrid in Vitro Binding, and Co-purification. Genetic Analysis of Yeast Spindle Pole Bodies. Methods for the Study of Centrosomes in Drosophilia during Embryogenesis. Methods for Identification of Centrosome-Associated Proteins. Reconstitution of Centrosome Microtubule Nucleation in Drosophilia. Reconstitution of Centrosome Microtubule Nucleation in Spisula.

In Vitro Approaches for the Study of Microtubule Nucleation at the Fission Yeast Spindle Pole Body. Gamma Tubulin and Microtubule Nucleation in Mammalian Cells. Gamma Tubulin in Plant Cells. Centrosomes and Parthenogenesis.

In Vitro Approaches for the Study of Molecular Motors in Aster Formation. Methods for the Study of Centrosome-Independent Spindle Assembly in Xenopus Extracts. Methods for the Study of Centrosome Reproduction in Mammalian Cells. Centrosome Reproduction in Xenopus Lysates. Centrosome Reproduction in Vitro: Mammalian Centrosomes in Xenopus Lysates. Identification of Centrosome Kinases. Methods for the Analysis of Centrosome Reproduction in Cancer Cells. Isolation of Centrosomes from Dictyostelium. Index. Volume in Series.

Readership

Researchers and graduate students in cell biology, developmental biology, genetics, and cancer research. Academic and research libraries, pharmaceutical and biotechnology companies, and private research facilities.


Details

No. of pages:
375
Language:
English
Copyright:
© Academic Press 2001
Published:
Imprint:
Academic Press
eBook ISBN:
9780080496627
eBook ISBN:
9780080925172
Paperback ISBN:
9780125439855

About the Editors

Robert Palazzo Editor

Affiliations and Expertise

Department of Molecular Biosciences, University of Kansas, Lawrence, U.S.A.

Trisha Davis Editor

Affiliations and Expertise

University of Washington, Seattle, U.S.A.