This book is the first devoted to centrifugal separation in biotechnology. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering.
Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation.
A comprehensive guide to centrifuges, their optimal development and operation in the biotechnology industry Applications for the separation of proteins, DNA, mitochondria, ribosomes, lysosomes and other cellular elements Provides detailed process information and data to assist in the development of particular processes from existing systems Explores the commercial applications of centrifuges in biotechnology Guidance on troubleshooting and optimizing centrifuges
chemical engineers, process & mechanical engineers, bioengineering professionals, pharmaceutical chemists, process industry R & D
1Introduction 1.1 Introduction 1.2 Centrifugal Separation and Filtration 1.2.1 Sedimenting Centrifuge 1.2.2 Filtering centrifuges 1.3 Pros and Cons of Filtration versus Centrifugation 1.4 Generic Flow Sheet for Biopharmaceutical Process 1.5 Other Centrifugal Separations 1.6 Inputs and Outputs of Centrifuge 1.7 Separation Metrics 1.7.1 Protein Yield 1.7.2 Centrate Suspended Solids. 1.7.3 Throughput Rate 1.7.4 Cell Viability 1.8 Summary
2Principles of Centrifugal Sedimentation 2.1 Introduction 2.2 Non-intuitive Phenomena 2.2.1 Pressure Distribution 2.2.2 Coriolis Effect 2.3 Intuitive Phenomena 2.3.1 Centrifugal Acceleration 2.3.2 Fluid in a Centrifuge Bowl not at Solid-Body Motion 2.3.3 Regimes of Sedimentation 2.3.4 Stokes’ Law 2.3.5 Settling with Concentrated Solids 2.4 Process Functions 2.5 Summary
3.Batch and Semi-Batch Centrifuges 3.1 Spintube 3.2 Centrifugal Filter 3.3 Ultracentrifuges 3.3.1 Analytical Ultracentrifuge 3.3.2 Preparative Ultracentrifuge 3.3.3 Centrifugal Elutriation 3.4 Tubular Centrifuge 3.4.1 General Tubular Bowl Geometry 3.4.2 Ribs and Solids Scraper 3.4.3 Plunger Cake Discharge 3.4.4 Submerged Hub 3.5 Summary
4.Disk Centrifuge 4.1 Lamella/Inclined Plate Settler 4.1.1 Inclined Plate Settler Principle 4.1.2 Complications in Inclined Plate Settler 4.2 Disk Stack Centrifuge 4.2.1 General Disk Geometry<BR id
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- © Academic Press 2007
- 17th July 2007
- Academic Press
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Dr. Wallace Woon-Fong Leung is Chair Professor of Innovative Products and Technologies, Department of Mechanical Engineering and Director, Research Institute of Innovative Products and Technologies, The Hong Kong Polytechnic University.
Chair Professor of Innovative Products and Technologies, Department of Mechanical Engineering; Director, Research Institute of Innovative Products and Technologies, The Hong Kong Polytechnic University, Hong Kong