Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."

Key Features

  • Comprehensively covers the topic of cellulases
  • International board of authors
  • Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

    Table of Contents


    1. Measurement of endo-1,4-b-glucanase
    2. Barry V. McCleary, Vincent McKie and Anna Draga

    3. Biomass Conversion determined via Fluorescent Cellulose Decay assay
    4. Bente Wischmann, Marianne Toft, Marco Malten and KC McFarland

    5. The analysis of saccharification in biomass using an automated high-throughput method
    6. Caragh Whitehead, Leonardo D. Gomez, Simon J. McQueen-Mason

    7. Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
    8. Ondrej Kosik, Jennifer Bromley, Marta Busse-Wicher, Zhinong Zhang & Paul Dupree

    9. Measuring processivity
    10. Svein J. Horn, Morten Sørlie, Kjell M. Vårum, Priit Väljamäe and Vincent G.H. Eijsink

    11. Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases
    12. Jens M. Eklöf, Marcus C. Ruda and Harry Brumer

    13. Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
    14. Maria J. Peña, Sami T. Tuomivaara, Breeanna R. Urbanowicz, Malcolm A. O’Neill and William S. York

    15. The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
    16. Shirley M. Roberts and Gideon J. Davies

    17. Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
    18. Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Merja Penttilä, Toshio Ando and Masahiro Samejima

    19. Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
    20. Mirjam Czjzek, Henri-Pierre


    No. of pages:
    © 2012
    Academic Press
    Print ISBN:
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