Cellular Immunology LabFax

Cellular Immunology LabFax

1st Edition - July 13, 1994

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  • Editors: B. Hames, D. Rickwood
  • eBook ISBN: 9781483297705

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LABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents the key information for a major subject in one place and so saves hours of searching. The authors and editors of each LABFAX volume have searched the original literature for the accurate data they know the subject specialist needs.Cellular Immunology Labfax is a detailed compendium of information on the tissues and cells of the immune system, cell lines and hybridomas, antigens and receptors, the major histocompatibility complex, soluble immunoregulatory molecules, in vivo procedures, important features of species used in immunological research, and much more.The contents of Cellular Immunology Labfax have been carefully chosen to complement the data presented in Immunochemistry Labfax. It is therefore the companion volume to this text.

Table of Contents

  • Contributors


    1. Laboratory Animals

    Welfare of Laboratory Animals

    Health Monitoring

    Anesthesia and Analgesia




    Animal Models in Immunology

    Induced Animal Models

    Spontaneous Animal Models

    Transplantation Immunology

    Graft versus Host Reaction

    Immunization of Animals for Antibody Production

    Polyclonal Antibody Production

    Monoclonal Antibody Production

    Antigen Purity and Dose


    Routes of Administration

    A Standard Procedure for the Production of Monoclonal Antibodies

    A Standard Procedure for the Production of Polyclonal Antibodies



    2. Tissues and Cells of the Immune System

    Primary Lymphoid Tissues


    Bursa of Fabricius

    Mammalian B Lymphopoiesis

    Secondary (Peripheral) Lymphoid Tissues

    Lymph Nodes


    Mucosa-Associated Lymphoid Tissue (MALT)

    Lymphocyte Recirculation and Homing

    Cells of the Immune Response


    Nonlymphoid Cells


    3. Leukocyte Development


    Lymphocyte Development

    B Cells

    T Cells

    Markers of Cellular Activation

    Leukocyte Lifespans

    Age Dependence of Lymphocyte Subpopulations

    Methods of Studying Lymphocyte Development


    4. Tissue and Cell Culture

    General Information

    Biosafety Cabinets

    Other Required Equipment

    Methods of Sterilization

    Biosafety Levels

    Tissue Culture Media

    Media Formulations

    Supplements and Media Modifications


    Organ Culture

    Long-Term Bone Marrow Cultures

    Thymic Organ Cultures

    Splenic Fragment Cultures

    Cell Isolation

    Methods of Tissue Dissociation

    Enumeration of Cell Number

    Washing and Removal of Dead Cells

    Cell Separation Techniques

    Dyes Used in FACS Analysis and Cell Sorting

    Cell Culture

    Cell Density

    Use of Feeder Cells

    Inactivation of Cells/Cell Substitutes

    Use of Culture Supernatants

    Polyclonal Activators

    Cell and Tissue Preservation




    5. Cell Lines and Hybridomas

    Cell Lines

    Viral- and Gene-Mediated Transformation

    Stromal Cell-Dependent B-Cell and T-Cell Lines

    Antigen-Specific T-Cell Lines

    Murine Helper T-Cell Lines

    Human Helper T-Cell Lines

    B-Cell Hybridomas

    General Aspects of Hybridoma Production


    Normal Lymphocytes

    Cell Fusion

    Selection of Hybridomas


    Culture of Hybridoma Clones

    Application of Monoclonal Antibodies

    T-Cell Hybridomas

    T-Lymphoma Lines

    Normal T Lymphocytes

    Fusion and Selection



    Definition and Purpose


    Verification of Clonality

    Cell-Line Suppliers


    6. Assays of Cellular Activity

    Measurement of Early Activation

    Phagocytosis and Microbicidal Activity

    Chemotaxis and Chemokinesis


    Cytokine Production

    Antibody Secretion


    Helper Function

    TH Lymphokine Secretion

    Supernatant Helper Function

    Limiting Dilution



    7. Cell-Surface Antigens



    8. Antigens Receptors


    General Features

    Immunoglobulin Heavy Chains

    Immunoglobulin Light Chains

    Class Differences between Ig Molecules

    T-Cell Receptor Chains

    Antigen Binding

    Genes Encoding Antigen Receptors


    Ig Heavy Chain Locus

    The Ig Light-Chain Gene Loci

    The TCR Gene Loci

    Rearrangement of Antigen-Receptor Genes

    Joining Signals and the 12/23 Rule

    Mechanisms of Joining

    Imprecision and Nucleotide Addition at Coding Joints

    Components of the Rearrangement Machinery

    Order of Rearrangement

    Allelic Exclusion

    Generation of Diversity

    Combinatorial and Junctional Diversity

    Somatic Hypermutation of Ig Genes

    Expression of Ig and TCR Genes

    Transcriptional Regulation of Ig and TCR Genes

    Isotype Switching in IgH Genes

    The Expressed V-Region Repertoire of TCR and Ig

    The Ig V-Region Repertoire

    The TCR V-Region Repertoire

    Assembly and Cell-Surface Transport of Antigen Receptors

    Components of the TCR Complex

    Assembly and Surface Transport of CD3/TCR Complexes

    Components of the Membrane-Bound Ig Complex

    Assembly and Surface Transport of Membrane-Bound Ig


    9. Antigen Processing and Presentation


    The Antigens

    Antigen-Presenting Cells

    The Interdigitating Dendritic Cell

    The Macrophage

    The B Cell

    Inhibitors of Antigen Processing

    The Antigen-Processing Pathway

    The Molecules



    10. The Major Histocompatibility Complex

    Molecules Encoded within the MHC

    Structure of Class I and class II Molecules

    Maps of H-2 and HLA Genes (and Other Species)

    Nomenclature of H-2 and HLA Alleles and Haplotypes

    Typing Methods


    11. Soluble Immunoregulatory Molecules




    Tumor Necrosis Factor-α and Lymphotoxin

    Transforming Growth Factors


    Colony-Stimulating Factors

    Soluble Cytokine Antagonists

    Soluble Adhesion Molecules

    Other Soluble Immune Regulatory Molecules



    12. Cellular Activation

    Ligands and Receptors Involved in Immune-Cell Activation

    Cell Lines Useful for Studying Signal Transduction

    Tyrosine Kinase Activation by Receptors on Cells of the Immune System

    PTK Substrates

    Tyrosine Kinase-Regulated Signal Transduction Pathways

    The Phospholipase C Pathway

    The Phosphatidylinositol 3-Kinase Pathway

    The p21ras Pathway

    The MAP Kinase Pathway

    Activation of Phospholipase C by G Protein-Linked Receptors

    Regulation of cAMP Levels

    Phosphatidylcholine Hydrolysis

    Glycosyl-Phosphatidylinositol (GPI) Hydrolysis

    Methods Used in Studying Signal Transduction



Product details

  • No. of pages: 260
  • Language: English
  • Copyright: © Academic Press 1994
  • Published: July 13, 1994
  • Imprint: Academic Press
  • eBook ISBN: 9781483297705

About the Series Editors

B. Hames

Affiliations and Expertise

Consultant Microbiologist, Freeman Hospital, Newcastle upon Tyne, UK

D. Rickwood

Affiliations and Expertise

University of Essex, Wivenhoe Park, Colchester

About the Series Volume Editor

Peter Delves

Affiliations and Expertise

University College London Medical School, Arthur Stanley House, London, U.K.

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