Cellular Immunology LabFax

Cellular Immunology LabFax

1st Edition - July 13, 1994

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  • Editors: B. Hames, D. Rickwood
  • eBook ISBN: 9781483297705

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Description

LABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents the key information for a major subject in one place and so saves hours of searching. The authors and editors of each LABFAX volume have searched the original literature for the accurate data they know the subject specialist needs.Cellular Immunology Labfax is a detailed compendium of information on the tissues and cells of the immune system, cell lines and hybridomas, antigens and receptors, the major histocompatibility complex, soluble immunoregulatory molecules, in vivo procedures, important features of species used in immunological research, and much more.The contents of Cellular Immunology Labfax have been carefully chosen to complement the data presented in Immunochemistry Labfax. It is therefore the companion volume to this text.

Table of Contents


  • Contributors

    Abbreviations

    1. Laboratory Animals

    Welfare of Laboratory Animals

    Health Monitoring

    Anesthesia and Analgesia

    Pre-Anesthesia

    Anesthesia

    Analgesia

    Animal Models in Immunology

    Induced Animal Models

    Spontaneous Animal Models

    Transplantation Immunology

    Graft versus Host Reaction

    Immunization of Animals for Antibody Production

    Polyclonal Antibody Production

    Monoclonal Antibody Production

    Antigen Purity and Dose

    Adjuvants

    Routes of Administration

    A Standard Procedure for the Production of Monoclonal Antibodies

    A Standard Procedure for the Production of Polyclonal Antibodies

    Bleeding

    References

    2. Tissues and Cells of the Immune System

    Primary Lymphoid Tissues

    Thymus

    Bursa of Fabricius

    Mammalian B Lymphopoiesis

    Secondary (Peripheral) Lymphoid Tissues

    Lymph Nodes

    Spleen

    Mucosa-Associated Lymphoid Tissue (MALT)

    Lymphocyte Recirculation and Homing

    Cells of the Immune Response

    Lymphocytes

    Nonlymphoid Cells

    References

    3. Leukocyte Development

    Hematopoiesis

    Lymphocyte Development

    B Cells

    T Cells

    Markers of Cellular Activation

    Leukocyte Lifespans

    Age Dependence of Lymphocyte Subpopulations

    Methods of Studying Lymphocyte Development

    References

    4. Tissue and Cell Culture

    General Information

    Biosafety Cabinets

    Other Required Equipment

    Methods of Sterilization

    Biosafety Levels

    Tissue Culture Media

    Media Formulations

    Supplements and Media Modifications

    Antibiotics

    Organ Culture

    Long-Term Bone Marrow Cultures

    Thymic Organ Cultures

    Splenic Fragment Cultures

    Cell Isolation

    Methods of Tissue Dissociation

    Enumeration of Cell Number

    Washing and Removal of Dead Cells

    Cell Separation Techniques

    Dyes Used in FACS Analysis and Cell Sorting

    Cell Culture

    Cell Density

    Use of Feeder Cells

    Inactivation of Cells/Cell Substitutes

    Use of Culture Supernatants

    Polyclonal Activators

    Cell and Tissue Preservation

    Fixation

    Cryopreservation

    References

    5. Cell Lines and Hybridomas

    Cell Lines

    Viral- and Gene-Mediated Transformation

    Stromal Cell-Dependent B-Cell and T-Cell Lines

    Antigen-Specific T-Cell Lines

    Murine Helper T-Cell Lines

    Human Helper T-Cell Lines

    B-Cell Hybridomas

    General Aspects of Hybridoma Production

    Myelomas

    Normal Lymphocytes

    Cell Fusion

    Selection of Hybridomas

    Screening

    Culture of Hybridoma Clones

    Application of Monoclonal Antibodies

    T-Cell Hybridomas

    T-Lymphoma Lines

    Normal T Lymphocytes

    Fusion and Selection

    Screening

    Cloning

    Definition and Purpose

    Procedures

    Verification of Clonality

    Cell-Line Suppliers

    References

    6. Assays of Cellular Activity

    Measurement of Early Activation

    Phagocytosis and Microbicidal Activity

    Chemotaxis and Chemokinesis

    Proliferation

    Cytokine Production

    Antibody Secretion

    Cytotoxicity

    Helper Function

    TH Lymphokine Secretion

    Supernatant Helper Function

    Limiting Dilution

    Apoptosis

    References

    7. Cell-Surface Antigens

    Introduction

    References

    8. Antigens Receptors

    Structure

    General Features

    Immunoglobulin Heavy Chains

    Immunoglobulin Light Chains

    Class Differences between Ig Molecules

    T-Cell Receptor Chains

    Antigen Binding

    Genes Encoding Antigen Receptors

    Organization

    Ig Heavy Chain Locus

    The Ig Light-Chain Gene Loci

    The TCR Gene Loci

    Rearrangement of Antigen-Receptor Genes

    Joining Signals and the 12/23 Rule

    Mechanisms of Joining

    Imprecision and Nucleotide Addition at Coding Joints

    Components of the Rearrangement Machinery

    Order of Rearrangement

    Allelic Exclusion

    Generation of Diversity

    Combinatorial and Junctional Diversity

    Somatic Hypermutation of Ig Genes

    Expression of Ig and TCR Genes

    Transcriptional Regulation of Ig and TCR Genes

    Isotype Switching in IgH Genes

    The Expressed V-Region Repertoire of TCR and Ig

    The Ig V-Region Repertoire

    The TCR V-Region Repertoire

    Assembly and Cell-Surface Transport of Antigen Receptors

    Components of the TCR Complex

    Assembly and Surface Transport of CD3/TCR Complexes

    Components of the Membrane-Bound Ig Complex

    Assembly and Surface Transport of Membrane-Bound Ig

    References

    9. Antigen Processing and Presentation

    Genetics

    The Antigens

    Antigen-Presenting Cells

    The Interdigitating Dendritic Cell

    The Macrophage

    The B Cell

    Inhibitors of Antigen Processing

    The Antigen-Processing Pathway

    The Molecules

    Conclusions

    References

    10. The Major Histocompatibility Complex

    Molecules Encoded within the MHC

    Structure of Class I and class II Molecules

    Maps of H-2 and HLA Genes (and Other Species)

    Nomenclature of H-2 and HLA Alleles and Haplotypes

    Typing Methods

    References

    11. Soluble Immunoregulatory Molecules

    Introduction

    Cytokines

    Interleukins

    Tumor Necrosis Factor-α and Lymphotoxin

    Transforming Growth Factors

    Interferons

    Colony-Stimulating Factors

    Soluble Cytokine Antagonists

    Soluble Adhesion Molecules

    Other Soluble Immune Regulatory Molecules

    Immunoglobulins

    References

    12. Cellular Activation

    Ligands and Receptors Involved in Immune-Cell Activation

    Cell Lines Useful for Studying Signal Transduction

    Tyrosine Kinase Activation by Receptors on Cells of the Immune System

    PTK Substrates

    Tyrosine Kinase-Regulated Signal Transduction Pathways

    The Phospholipase C Pathway

    The Phosphatidylinositol 3-Kinase Pathway

    The p21ras Pathway

    The MAP Kinase Pathway

    Activation of Phospholipase C by G Protein-Linked Receptors

    Regulation of cAMP Levels

    Phosphatidylcholine Hydrolysis

    Glycosyl-Phosphatidylinositol (GPI) Hydrolysis

    Methods Used in Studying Signal Transduction

    References

    Index

Product details

  • No. of pages: 260
  • Language: English
  • Copyright: © Academic Press 1994
  • Published: July 13, 1994
  • Imprint: Academic Press
  • eBook ISBN: 9781483297705

About the Series Editors

B. Hames

Affiliations and Expertise

Consultant Microbiologist, Freeman Hospital, Newcastle upon Tyne, UK

D. Rickwood

Affiliations and Expertise

University of Essex, Wivenhoe Park, Colchester

About the Series Volume Editor

Peter Delves

Affiliations and Expertise

University College London Medical School, Arthur Stanley House, London, U.K.

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