Description

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.

Key Features

* Updates the numerous technological innovations that have improved the capabilities of electron microscopy * Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging * Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

Readership

Researchers in various fields of biology who utilize electron microscopy.

Table of Contents

Introduction: McIntosh - A new era in electron microscopy of cells Preparative Methods for Cellular Electron Microscopy 1. The physics of rapid cooling and its implications for cryo-immobilization of cells 2. Cryopreparation Methods for Electron Microscopy of Selected Model Systems 3. Cryo-preparation methodology for plant cell biology 4. Correlative Light and Electron Microscopy of Early C. elegans Embryos in Mitosis Imaging Fixed Cells in Three Dimensions 5. Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography 6. Whole Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography 7. Electron Microscopy of archaea 8. Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography 9. Visualization of Membrane-Cytoskeletal Interactions during Plant Cytokinesis 10. Electron Tomographic Methods for Studying the Chemical Synapse 11. Using electron microscopy to understand functional mechanisms of chromosome alignment on the mitotic spindle 12. EM Analysis of the Leading Edge in Migrating Cells 13. Imaging actomyosin in situ Imaging Frozen-Hydrated Cells and Cell Parts 14. Electron tomography of bacterial chemotaxis receptor assemblies 15. How to “read” a vitreous section 16. Single Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction 17. Electron microscopy of microtubule-based cytoskeletal machinery 18. Reconstructing the endocytotic machinery Localizing Macromolecules in Cells 19. 3-D Immuno-locali

Details

No. of pages:
880
Language:
English
Copyright:
© 2007
Published:
Imprint:
Academic Press
eBook ISBN:
9780080475035
Print ISBN:
9780123706478

About the editor

Reviews

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.