Cellular Electron Microscopy

Cellular Electron Microscopy

1st Edition - February 22, 2007

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  • Editor: J. McIntosh
  • eBook ISBN: 9780080475035
  • Hardcover ISBN: 9780123706478

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Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.

Key Features

  • Updates the numerous technological innovations that have improved the capabilities of electron microscopy
  • Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging
  • Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going


Researchers in various fields of biology who utilize electron microscopy.

Table of Contents

  • Introduction: McIntosh - A new era in electron microscopy of cells

    Preparative Methods for Cellular Electron Microscopy
    1. The physics of rapid cooling and its implications for cryo-immobilization of cells
    2. Cryopreparation Methods for Electron Microscopy of Selected Model Systems
    3. Cryo-preparation methodology for plant cell biology
    4. Correlative Light and Electron Microscopy of Early C. elegans Embryos in Mitosis
    Imaging Fixed Cells in Three Dimensions
    5. Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography
    6. Whole Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography
    7. Electron Microscopy of archaea
    8. Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography
    9. Visualization of Membrane-Cytoskeletal Interactions during Plant Cytokinesis
    10. Electron Tomographic Methods for Studying the Chemical Synapse
    11. Using electron microscopy to understand functional mechanisms of chromosome alignment on the mitotic spindle
    12. EM Analysis of the Leading Edge in Migrating Cells
    13. Imaging actomyosin in situ

    Imaging Frozen-Hydrated Cells and Cell Parts
    14. Electron tomography of bacterial chemotaxis receptor assemblies
    15. How to “read” a vitreous section
    16. Single Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction
    17. Electron microscopy of microtubule-based cytoskeletal machinery
    18. Reconstructing the endocytotic machinery

    Localizing Macromolecules in Cells
    19. 3-D Immuno-localization with plastic sections
    20. EM analysis of viral morphogenesis
    21. Electron tomography of immuno-labeled cryosections
    22. Visualizing macromolecules with fluoronanogold: from photon microscopy to electron tomography
    23. Markers for correlated light and electron microscopy
    24. Localizing Specific Elements Bound to Macromolecules by EFTEM
    25. Localization of protein complexes by pattern recognition

    Aspects of Data Collection and Analysis Instrumentation for image acquisition
    26. Application of Energy Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples
    27. Optimization of Image Collection for Cellular Electron Microscopy
    28. Future Directions for Camera Systems in Electron Microscopy

    Computational methods for the analysis of cellular data
    29. Structure determination in situ by averaging of tomograms
    30. Methods for image segmentation in cellular tomography
    31. Database Resources for Cellular Electron Microscopy

Product details

  • No. of pages: 880
  • Language: English
  • Copyright: © Academic Press 2007
  • Published: February 22, 2007
  • Imprint: Academic Press
  • eBook ISBN: 9780080475035
  • Hardcover ISBN: 9780123706478

About the Editor

J. McIntosh

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