Cellular and Molecular Procedures in Developmental Biology
Free Global Shipping
No minimum orderDescription
This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures.
Key Features
- Provides straightforward, current protocols and critical appraisals
- Includes diverse analysis of cellular and molecular techniques
- Presents everything from whole-organ cultures to electrophysiological approaches
- Details a variety of methods for interfering with gene function in various species
- Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis
Readership
Graduate students and researchers in developmental, molecular, and cell biology, as well as genetics, zoology, agriculture, and neuroscience.
Table of Contents
- E. Dupin, C. Ziller, and N.M. Le Douarin, The Avian Embryo as a Model in Developmental Studies.
A.V. Morales and F. de Pablo, Inhibition of Gene Expression by Antisense Oligonucleotides in Chick Embryos in Vitro and in Vivo.
C.L. Cepko, E. Ryder, C. Austin, J. Golden, and S. Fields-Berry, Lineage Analysis Using Retroviral Vectors.
G. Lagna and A. Hemmati-Brivanlou, Use of Dominant/Negative Constructs to Modulate Gene Expression.
M. Torres, The Use of Embryonic Stem Cells for the Genetic Manipulation of the Mouse.
J.J. Garrido, T. Schimmang, J. Represa, and F. Giraldez, Organoculture of Otic Vesicle and Ganglion.
E.J. de la Rosa, B. Diaz, and F. de Pablo, Organoculture of the Chick Embryonic Neuroretina.
C.E. Krull and P.M. Kulesa, Embryonic Explant and Slice Preparations for Studies of Cell Migration and Axon Guidance.
A.V. Holst and H. Roher, Culture of Avian Sympathetic Neurons.
M-J. Fann and P.H. Patterson, Analysis of Gene Expression in Cultured Primary Neurons.
S. Nakagawa, H. Matsunami, and M. Takeichi, Selective Aggregation Assays for Embryonic Brain Cell and Cell Lines.
J. Serna, B. Pimentel, and E.J. de la Rosa, Flow Cytometric Analysis of Whole Organs and Embryos.
C.D. Stern, Detection of Multiple Gene Products Simultaneously by in Situ Hybridization and Immunohistochemistry in Whole Mounts of Avian Embryos.
C. Dulac, Differential Cloning from Single Cell cDNA Libraries.
F. Feiguin, S. Llamazares, and C. Gonzalez, Methods in Drosophila Cell Cycle Biology.
J. Lerma, M. Morales, and M. de los Angeles Vincente, Single CNS Neurons in Culture.
M. Martinez-Padron and A. Ferrus, Patch-Clamp Recordings from Drosophila Presynaptic Terminals.
N.J. McCarthy and G.I. Evan, Methods for Detecting and Quantifying Apoptosis.
Subject Index.
Contents of Previous Volumes.
Product details
- No. of pages: 329
- Language: English
- Copyright: © Academic Press 1997
- Published: October 10, 1997
- Imprint: Academic Press
- eBook ISBN: 9780080584591
About the Serial Editors
Roger Pedersen
Affiliations and Expertise
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, U.S.A.
Gerald Schatten
Affiliations and Expertise
University of Pittsburgh School of Medicine, PA, U.S.A.
About the Serial Volume Editors
Flora de Pablo
Affiliations and Expertise
Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain
Alberto Ferrus
Affiliations and Expertise
Instituto Cajal, C.S.I.C., Madrid, Spain
Claudio Stern
Claudio Stern is at Department of Cell and Developmental Biology, UCL, London, UK
Affiliations and Expertise
UCL, London, UK
Ratings and Reviews
There are currently no reviews for "Cellular and Molecular Procedures in Developmental Biology"