Cellular and Molecular Procedures in Developmental Biology

Cellular and Molecular Procedures in Developmental Biology

1st Edition - October 10, 1997

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  • Editors: Roger Pedersen, Gerald Schatten
  • eBook ISBN: 9780080584591

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This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures.

Key Features

  • Provides straightforward, current protocols and critical appraisals
  • Includes diverse analysis of cellular and molecular techniques
  • Presents everything from whole-organ cultures to electrophysiological approaches
  • Details a variety of methods for interfering with gene function in various species
  • Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis


Graduate students and researchers in developmental, molecular, and cell biology, as well as genetics, zoology, agriculture, and neuroscience.

Table of Contents

  • E. Dupin, C. Ziller, and N.M. Le Douarin, The Avian Embryo as a Model in Developmental Studies.
    A.V. Morales and F. de Pablo, Inhibition of Gene Expression by Antisense Oligonucleotides in Chick Embryos in Vitro and in Vivo.
    C.L. Cepko, E. Ryder, C. Austin, J. Golden, and S. Fields-Berry, Lineage Analysis Using Retroviral Vectors.
    G. Lagna and A. Hemmati-Brivanlou, Use of Dominant/Negative Constructs to Modulate Gene Expression.
    M. Torres, The Use of Embryonic Stem Cells for the Genetic Manipulation of the Mouse.
    J.J. Garrido, T. Schimmang, J. Represa, and F. Giraldez, Organoculture of Otic Vesicle and Ganglion.
    E.J. de la Rosa, B. Diaz, and F. de Pablo, Organoculture of the Chick Embryonic Neuroretina.
    C.E. Krull and P.M. Kulesa, Embryonic Explant and Slice Preparations for Studies of Cell Migration and Axon Guidance.
    A.V. Holst and H. Roher, Culture of Avian Sympathetic Neurons.
    M-J. Fann and P.H. Patterson, Analysis of Gene Expression in Cultured Primary Neurons.
    S. Nakagawa, H. Matsunami, and M. Takeichi, Selective Aggregation Assays for Embryonic Brain Cell and Cell Lines.
    J. Serna, B. Pimentel, and E.J. de la Rosa, Flow Cytometric Analysis of Whole Organs and Embryos.
    C.D. Stern, Detection of Multiple Gene Products Simultaneously by in Situ Hybridization and Immunohistochemistry in Whole Mounts of Avian Embryos.
    C. Dulac, Differential Cloning from Single Cell cDNA Libraries.
    F. Feiguin, S. Llamazares, and C. Gonzalez, Methods in Drosophila Cell Cycle Biology.
    J. Lerma, M. Morales, and M. de los Angeles Vincente, Single CNS Neurons in Culture.
    M. Martinez-Padron and A. Ferrus, Patch-Clamp Recordings from Drosophila Presynaptic Terminals.
    N.J. McCarthy and G.I. Evan, Methods for Detecting and Quantifying Apoptosis.
    Subject Index.
    Contents of Previous Volumes.

Product details

  • No. of pages: 329
  • Language: English
  • Copyright: © Academic Press 1997
  • Published: October 10, 1997
  • Imprint: Academic Press
  • eBook ISBN: 9780080584591

About the Serial Editors

Roger Pedersen

Affiliations and Expertise

Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, U.S.A.

Gerald Schatten

Affiliations and Expertise

University of Pittsburgh School of Medicine, PA, U.S.A.

About the Serial Volume Editors

Flora de Pablo

Affiliations and Expertise

Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain

Alberto Ferrus

Affiliations and Expertise

Instituto Cajal, C.S.I.C., Madrid, Spain

Claudio Stern

Claudio Stern is at Department of Cell and Developmental Biology, UCL, London, UK

Affiliations and Expertise

UCL, London, UK

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