This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures.

Key Features

@introbul:Key Features @bul:* Provides straightforward, current protocols and critical appraisals * Includes diverse analysis of cellular and molecular techniques * Presents everything from whole-organ cultures to electrophysiological approaches * Details a variety of methods for interfering with gene function in various species * Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis


Graduate students and researchers in developmental, molecular, and cell biology, as well as genetics, zoology, agriculture, and neuroscience.

Table of Contents

E. Dupin, C. Ziller, and N.M. Le Douarin, The Avian Embryo as a Model in Developmental Studies. A.V. Morales and F. de Pablo, Inhibition of Gene Expression by Antisense Oligonucleotides in Chick Embryos in Vitro and in Vivo. C.L. Cepko, E. Ryder, C. Austin, J. Golden, and S. Fields-Berry, Lineage Analysis Using Retroviral Vectors. G. Lagna and A. Hemmati-Brivanlou, Use of Dominant/Negative Constructs to Modulate Gene Expression. M. Torres, The Use of Embryonic Stem Cells for the Genetic Manipulation of the Mouse. J.J. Garrido, T. Schimmang, J. Represa, and F. Giraldez, Organoculture of Otic Vesicle and Ganglion. E.J. de la Rosa, B. Diaz, and F. de Pablo, Organoculture of the Chick Embryonic Neuroretina. C.E. Krull and P.M. Kulesa, Embryonic Explant and Slice Preparations for Studies of Cell Migration and Axon Guidance. A.V. Holst and H. Roher, Culture of Avian Sympathetic Neurons. M-J. Fann and P.H. Patterson, Analysis of Gene Expression in Cultured Primary Neurons. S. Nakagawa, H. Matsunami, and M. Takeichi, Selective Aggregation Assays for Embryonic Brain Cell and Cell Lines. J. Serna, B. Pimentel, and E.J. de la Rosa, Flow Cytometric Analysis of Whole Organs and Embryos. C.D. Stern, Detection of Multiple Gene Products Simultaneously by in Situ Hybridization and Immunohistochemistry in Whole Mounts of Avian Embryos. C. Dulac, Differential Cloning from Single Cell cDNA Libraries. F. Feiguin, S. Llamazares, and C. Gonzalez, Methods in Drosophila Cell Cycle Biology. J. Lerma, M. Morales, and M. de los Angeles Vincente, Single CNS Neurons in Culture. M. Martinez-Padron and A. Ferrus<


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© 1998
Academic Press
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