Cells and Tissues - 2nd Edition - ISBN: 9781483230467, 9781483270777

Cells and Tissues

2nd Edition

Editors: Arthur W. Pollister
eBook ISBN: 9781483270777
Imprint: Academic Press
Published Date: 1st January 1966
Page Count: 422
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Description

Physical Techniques in Biological Research, Volume III, Part A: Cells and Tissues focuses on improvements and innovations in physical techniques involved in biological research on cells and tissues. The selection first offers information on phase contrast and interference microscopy in cytology and birefringence and dichroism of cells and tissues. Topics include observational methods and results, quantitative techniques, nerve and muscle fibers, chloroplasts, cytoplasm, and nucleus. The book then examines X-ray microscopy and X-ray absorption analysis, including theoretical considerations of X-ray microscopy of biological materials and absorption and production of X-rays.
The publication ponders on microtomy and manometric techniques for single cells and electron microscopy of microorganisms. Discussions focus on Cartesian diver respirometers, capillary respirometers, preliminary preparation of materials for drying, and techniques for studying dried specimens.
The selection is highly recommended for students and readers interested in the physical techniques used in biological research.

Table of Contents


Contents

List of Contributors v

Preface to First Edition of Volume III

Preface to Second Edition

Chapter 1 Phase Contrast and Interference Microscopy in Cytology

I. Introduction

II. Observational Methods and Results

III. Quantitative Techniques

References

Chapter 2 Birefringence and Dichroism of Cells and Tissue

I. Introduction

II. Plant Cell Walls

III. Cytoplasm

IV. Chloroplasts

V. Nerve Fibers

VI. Muscle Fibers

VII. Nucleus

References

Chapter 3 X-Ray Microscopy and X-Ray Absorption Analysis

I. Introduction

II. Production of X-Rays

III. Absorption of X-Rays

IV. The Formation of X-Ray Images

V. Theoretical Considerations of X-Ray Microscopy of Biological Materials

VI. Equipment

VII. Preparation of the Biological Material for Historadiography and a Discussion of Errors

VIII. Some Applications of Microradiography

References

Chapter 4 Microtomy

I. Introduction

II. The Specimen and Embedding

III. Microtomes

IV. Knives and Knife Sharpening

V. Measurement of Section Thickness as a Criterion of Over-all Performance

VI. Resolution and Section Thickness

References

Chapter 5 Manometric Techniques for Single Cells

I. Introduction

II. Capillary Respirometers

III. Cartesian Diver Respirometers

References

Chapter 6 Electron Microscopy of Microorganisms

I. Introduction

II. Preliminary Preparation of Materials for Drying

III. Techniques for Drying Specimens

IV. Techniques for Studying Dried Specimens

V. Conclusions

References

Author index

Subject Index


Details

No. of pages:
422
Language:
English
Copyright:
© Academic Press 1966
Published:
Imprint:
Academic Press
eBook ISBN:
9781483270777

About the Editor

Arthur W. Pollister