Cells and Tissues

Cells and Tissues

2nd Edition - January 1, 1966

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  • Editor: Arthur W. Pollister
  • eBook ISBN: 9781483270777

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Physical Techniques in Biological Research, Volume III, Part A: Cells and Tissues focuses on improvements and innovations in physical techniques involved in biological research on cells and tissues. The selection first offers information on phase contrast and interference microscopy in cytology and birefringence and dichroism of cells and tissues. Topics include observational methods and results, quantitative techniques, nerve and muscle fibers, chloroplasts, cytoplasm, and nucleus. The book then examines X-ray microscopy and X-ray absorption analysis, including theoretical considerations of X-ray microscopy of biological materials and absorption and production of X-rays. The publication ponders on microtomy and manometric techniques for single cells and electron microscopy of microorganisms. Discussions focus on Cartesian diver respirometers, capillary respirometers, preliminary preparation of materials for drying, and techniques for studying dried specimens. The selection is highly recommended for students and readers interested in the physical techniques used in biological research.

Table of Contents

  • Contents

    List of Contributors v

    Preface to First Edition of Volume III

    Preface to Second Edition

    Chapter 1 Phase Contrast and Interference Microscopy in Cytology

    I. Introduction

    II. Observational Methods and Results

    III. Quantitative Techniques


    Chapter 2 Birefringence and Dichroism of Cells and Tissue

    I. Introduction

    II. Plant Cell Walls

    III. Cytoplasm

    IV. Chloroplasts

    V. Nerve Fibers

    VI. Muscle Fibers

    VII. Nucleus


    Chapter 3 X-Ray Microscopy and X-Ray Absorption Analysis

    I. Introduction

    II. Production of X-Rays

    III. Absorption of X-Rays

    IV. The Formation of X-Ray Images

    V. Theoretical Considerations of X-Ray Microscopy of Biological Materials

    VI. Equipment

    VII. Preparation of the Biological Material for Historadiography and a Discussion of Errors

    VIII. Some Applications of Microradiography


    Chapter 4 Microtomy

    I. Introduction

    II. The Specimen and Embedding

    III. Microtomes

    IV. Knives and Knife Sharpening

    V. Measurement of Section Thickness as a Criterion of Over-all Performance

    VI. Resolution and Section Thickness


    Chapter 5 Manometric Techniques for Single Cells

    I. Introduction

    II. Capillary Respirometers

    III. Cartesian Diver Respirometers


    Chapter 6 Electron Microscopy of Microorganisms

    I. Introduction

    II. Preliminary Preparation of Materials for Drying

    III. Techniques for Drying Specimens

    IV. Techniques for Studying Dried Specimens

    V. Conclusions


    Author index

    Subject Index

Product details

  • No. of pages: 422
  • Language: English
  • Copyright: © Academic Press 1966
  • Published: January 1, 1966
  • Imprint: Academic Press
  • eBook ISBN: 9781483270777

About the Editor

Arthur W. Pollister

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