Cell-wide Metabolic Alterations Associated with Malignancy - 1st Edition - ISBN: 9780128013298, 9780128014837

Cell-wide Metabolic Alterations Associated with Malignancy, Volume 543

1st Edition

Serial Volume Editors: Lorenzo Galluzzi Guido Kroemer
eBook ISBN: 9780128014837
Hardcover ISBN: 9780128013298
Imprint: Academic Press
Published Date: 15th June 2014
Page Count: 384
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Table of Contents

  • Preface
    • Acknowledgments
  • Chapter One: Methods to Measure Cytoplasmic and Mitochondrial Ca2 + Concentration Using Ca2 +-Sensitive Dyes
    • Abstract
    • 1 Introduction
    • 2 Experimental Components and Considerations
    • 3 Isolation and Differentiation of Naïve CD4+ CD25− T Cells from Spleen and Lymph Nodes of Wild-Type and Orai1−/− Mice
    • 4 Mitochondrial Ca2 + Measurement
    • 5 Data Handling and Processing
    • 6 Notes
    • 7 Conclusion
    • Acknowledgments
  • Chapter Two: Methods to Measure Intracellular Ca2 + Fluxes with Organelle-Targeted Aequorin-Based Probes
    • Abstract
    • 1 Introduction
    • 2 Experimental Components and Considerations
    • 3 Application Examples
    • 4 Aequorin Improvement by Fusion with GFP
    • 5 Monitoring Ca2 + Fluxes in Living Organism by BRET
    • Acknowledgments
  • Chapter Three: Measuring Baseline Ca2 + Levels in Subcellular Compartments Using Genetically Engineered Fluorescent Indicators
    • Abstract
    • 1 Introduction
    • 2 Evolution of Recombinant Ca2 + Probes and Organelle Targeting
    • 3 Enhancing the Fluorophores
    • 4 Modulating Ca2 +-Binding Properties
    • 5 Developing Organelle Targeting
    • 6 Quantitative Considerations of Measurements of Organelle-Targeted Probes
    • 7 Experimental Protocols
    • 8 Semiquantitative Imaging of Basal Mitochondrial [Ca2 +] with a Novel Mitochondria-Targeted GCaMP6m (2mtGCaMP6m)
    • 9 Semiquantitative Imaging of Agonist-Induced Mitochondrial Ca2 + Signals in Primary Neurons Using the Low-Affinity 2mtD4cpv Probe
    • 10 Semiquantitative Imaging of Basal, Steady-State [Ca2 +] in the ER
    • 11 Concluding Remarks
  • Chapter Four: Autophagy and Autophagic Flux in Tumor Cells
    • Abstract
    • 1 Introduction
    • 2 Autophagy Initiation and Autophagic Flux
    • 3 Autophagic Flux and Degradation
    • 4 Conclusion
    • Acknowledgments
  • Chapter Five: Methods to Assess Autophagy In Situ—Transmission Electron Microscopy Versus Immunohistochemistry
    • Abstract
    • 1 Introduction
    • 2 Transmission Electron Microscopy
    • 3 Immunohistochemistry
    • 4 Concluding Remarks
    • Acknowledgments
  • Chapter Six: Methods to Measure the Enzymatic Activity of PI3Ks
    • Abstract
    • 1 Introduction
    • 2 Analysis of PI3K Lipid Kinase Activity in Immunoprecipitate
    • 3 Analysis of the Activity of Small Molecule PI3K Inhibitors
    • 4 Analysis of the Localized Synthesis of PI3K Lipid Products
    • 5 Materials
    • Acknowledgments
  • Chapter Seven: Luciferase-Based Reporter to Monitor the Transcriptional Activity of the SIRT3 Promoter
    • Abstract
    • 1 Introduction
    • 2 Protocol for Construction of Reporter Plasmid
    • 3 Assaying SIRT3 Promoter Activity with the Construct
    • 4 Verifying Reporter Results with Complementary Methods
    • 5 Summary
    • Acknowledgments
  • Chapter Eight: Metabolomic Profiling of Cultured Cancer Cells
    • Abstract
    • 1 Introduction
    • 2 Cell Culture, Treatments, and Sample Collection
    • 3 Chromatography, Mass Spectrometry, and Data Analysis
    • 4 Concluding Remarks
    • 5 Notes
    • Acknowledgments
  • Chapter Nine: Pulsed Stable Isotope-Resolved Metabolomic Studies of Cancer Cells
    • Abstract
    • 1 Introduction
    • 2 Experimental Procedures
    • 3 Data Analysis
    • 4 Outlook
  • Chapter Ten: Single-Cell Imaging for the Study of Oncometabolism
    • Abstract
    • 1 Introduction
    • 2 Methods
    • 3 MAMS Arrays
  • Chapter Eleven: Study of Cellular Oncometabolism via Multidimensional Protein Identification Technology
    • Abstract
    • 1 Introduction
    • 2 Sample Protocol
    • 3 Data Processing
    • 4 Example Applications of MuDPIT
    • 5 Summary
  • Chapter Twelve: In Vivo Quantitative Proteomics for the Study of Oncometabolism
    • Abstract
    • 1 Introduction
    • 2 Sample Preparation
    • 3 MS Sample Preparation
    • 4 Instruments
    • 5 MS Data Analysis
    • 6 Additional Considerations
    • 7 Conclusions
  • Chapter Thirteen: Metabolomic Profiling of Neoplastic Lesions in Mice
    • Abstract
    • 1 Introduction
    • 2 Metabolic Profiling and Cancer Biology
    • 3 Techniques Applied in Metabolomic Profiling of Neoplastic Lesions in Mice
    • 4 Conclusion
  • Chapter Fourteen: Metabolomic Profiling of Tumor-Bearing Mice
    • Abstract
    • 1 Introduction
    • 2 Mouse Models
    • 3 Sample Preparation and Mass Spectrometry Analysis (Fig. 14.3)
    • 4 Statistics
    • 5 Biomarker Validation
    • 6 Summary
  • Chapter Fifteen: Metabolomic Studies of Patient Material by High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
    • Abstract
    • 1 MAS-NMR Spectroscopy as a Tool for Metabolic Profiling
    • 2 MAS-NMR Versus Solution-State NMR Spectroscopy of Tumors
    • 3 MAS-NMR Tumor Profiling in Clinical Studies
    • 4 Metabolic Perturbations in Tumors Detected by MAS-NMR Spectroscopy
    • 5 Protocol for the Analysis of Tissue by 1H MAS-NMR Spectroscopy
  • Chapter Sixteen: Analysis of Metabolomic Profiling Data Acquired on GC–MS
    • Abstract
    • 1 Introduction
    • 2 Spectrum Deconvolution
    • 3 Metabolite Identification
    • 4 Metabolite Quantification
    • 5 Association Network Analysis
    • 6 Metabolic Pathway Analysis
    • Acknowledgments
  • Author Index
  • Subject Index

Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo.   Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as "yet another" general hallmark of cancer, accumulating evidence suggests that the metabolic alterations of each neoplasm rather represent a molecular signature that intimately accompanies, and hence cannot be severed from, all facets of malignant transformation. 

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers research methods in biomineralization science
  • Contains sections on such topics providing a a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo.

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.


Details

No. of pages:
384
Language:
English
Copyright:
© Academic Press 2014
Published:
Imprint:
Academic Press
eBook ISBN:
9780128014837
Hardcover ISBN:
9780128013298

About the Serial Volume Editors

Lorenzo Galluzzi Serial Volume Editor

Lorenzo Galluzzi received his Ph.D. in 2008 from the University of Paris Sud/Paris XI (France), and now works as a research manager in the laboratory of Guido Kroemer. He is particularly fascinated by several aspects of mitochondrial cell death, autophagy, cancer cell metabolism and tumour immunology. He has published more than 270 articles in peer-reviewed scientific journals, and is currently the 6th and youngest of the 30 most-cited European cell biologists (relative to the period 2007-2013).

Affiliations and Expertise

Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

Guido Kroemer Serial Volume Editor

Guido Kroemer got his M.D. in 1985 from the University of Innsbruck, Austria, and his Ph.D. in molecular biology in 1992 from the Autonomous University of Madrid, Spain. He is currently Professor at the Faculty of Medicine of the University of Paris Descartes/Paris V, Director of the INSERM research team ‘Apoptosis, Cancer and Immunity’, Director of the Metabolomics and Cell Biology platforms of the Gustave Roussy Cancer Campus, and Practitioner at the Hôpital Européen George Pompidou (Paris, France). He is also the Director of the Paris Alliance of Cancer Research Institutes (PACRI) and the Labex 'Immuno-Oncology'. Dr. Kroemer is best known for the discoveries that mitochondrial membrane permeabilization constitutes a decisive step in regulated cell death; that autophagy is a cytoprotective mechanism with lifespan-extending effects; and that anticancer therapies are successful only if they stimulate tumour-targeting immune responses. He is currently the most-cited cell biologist in Europe (relative to the period 2007-2013), and he has received the Descartes Prize of the European Union, the Carus Medal of the Leopoldina, the Dautrebande Prize of the Belgian Royal Academy of Medicine, the Léopold Griffuel Prize of the French Association for Cancer Research, the Mitjavile prize of the French National Academy of Medicine and a European Research Council Advanced Investigator Award.

Affiliations and Expertise

INSERM Cordeliers Research Cancer Paris; Hopital Europeen Georges Pompidou; Universite Paris Descartes, France