Cell, Tissue, and Organ Cultures in Neurobiology - 1st Edition - ISBN: 9780122504501, 9780323143158

Cell, Tissue, and Organ Cultures in Neurobiology

1st Edition

Editors: S. Fedoroff
eBook ISBN: 9780323143158
Imprint: Academic Press
Published Date: 1st January 1977
Page Count: 708
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Description

Cell, Tissue, and Organ Cultures in Neurobiology emerged from an international workshop held at the University of Saskatchewan in March 1977. This book reviews the uses of cell, tissue, and organ cultures in neurobiological research. It brings together an interdisciplinary perspective from morphology, biochemistry, pharmacology, endocrinology, embryology, and genetics. The book is organized into seven parts. Part I contains papers on the characteristics of differentiated cells. Part II presents studies on cell differentiation in primary cultures. Part III deals with studies on cell cultures and cell strains. Part IV focuses on phenotypic cell expression. Part V examines various cellular interactions. Part VI covers studies on nutrition while Part VII takes up applications of cell tissue and organ cultures in neurobiology. The book is directed toward tissue culturists concerned with the nervous system, as well as all neurobiologists, cell biologists, and embryologists interested in learning how neural cells and tissues behave in cultures and what has been learned about the nervous system using tissue culture methods, including the applicability of tissue cultures to the study of cell differentiation.

Table of Contents


List of Contributors

Preface

Introduction

Characteristics of Differentiated Cells

Neuroglia—From the Subventricular Precursor to the Mature Cell

Biochemistry of Glial Cells

Physiological and Pharmacological Studies on Central Synaptic Transmission

Characteristics of Neurons in Tissue Culture

Differentiation of Cells in Primary Cultures

Differentiation of Cells in Organ Cultures

Differentiation of Cells in Primary Cultures: Myelination

Synapse Formation in Neural Tissue Cultures

Dissociated Brain Cells in Primary Cultures

Tracing Glial Cell Lineages by Colony Formation in Primary Cultures

Differentiation of Glioblasts under the Influence of Glia Maturation Factor

Neural Cell Isolation and Identification

Cell Lines and Cell Strains

Primary Cultures, Cell Lines, and Cell Strains: Terminology and Characteristics

Glial Cell Lines

Neuronal Cell Lines

Phenotypic Cell Expression

Genetic Control of Neural Development

Specific Protein Species Expressed by Differentiating Neuroblastoma, Glioma, and Hybrid Cells in Culture. Evidence for Neuroblast-Glioblast Reciprocal Genetic Regulation

Immunochemical Markers in Primary Cultures and in Cell Lines

Transmitters, Putative Transmitters, and Transmitter-Related Enzymes Studied in Cultured Cell Systems

Differences between Astrocytes in Primary Cultures and Glial Cell Lines in Uptake and Metabolism of Putative Amino Acid Transmitters

Role of Cyclic Nucleotide in the Differentiation of Nerve Cells

Hormonal Effects on Differentiation in Neural Cultures

Cell Interactions

Cell Aggregation and Recognition in the Self-Assembly of Brain Tissues

Organization of Pathways in Cultures of Mouse Cerebellum

Cell and Organotypic Culture Studies of Neurological Mutations Affecting Structural Development

Neurosecretion in Cultured Systems

A Putative Precursor to Neurophysins and Neurohypophyseal Hormones

Neuroblast-Glioblast Interactions: Ecto-Enzymes

Nutrition

Hormones and Growth Factors in Cell Cultures: Problems and Perspectives

Nutritional Requirements of Cells in Culture, with Special Reference to Neural Cells

Use of Cell Tissue and Organ Cultures in Neurobiology

Cell and Tissue Differentiation in Cultures: Discussion

Cultured Neural Cells and Tissues as Models of Their In Vivo Counterparts: Discussion

Index


Details

No. of pages:
708
Language:
English
Copyright:
© Academic Press 1977
Published:
Imprint:
Academic Press
eBook ISBN:
9780323143158

About the Editor

S. Fedoroff

Ratings and Reviews