
Cell, Tissue, and Organ Cultures in Neurobiology
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Cell, Tissue, and Organ Cultures in Neurobiology emerged from an international workshop held at the University of Saskatchewan in March 1977. This book reviews the uses of cell, tissue, and organ cultures in neurobiological research. It brings together an interdisciplinary perspective from morphology, biochemistry, pharmacology, endocrinology, embryology, and genetics. The book is organized into seven parts. Part I contains papers on the characteristics of differentiated cells. Part II presents studies on cell differentiation in primary cultures. Part III deals with studies on cell cultures and cell strains. Part IV focuses on phenotypic cell expression. Part V examines various cellular interactions. Part VI covers studies on nutrition while Part VII takes up applications of cell tissue and organ cultures in neurobiology. The book is directed toward tissue culturists concerned with the nervous system, as well as all neurobiologists, cell biologists, and embryologists interested in learning how neural cells and tissues behave in cultures and what has been learned about the nervous system using tissue culture methods, including the applicability of tissue cultures to the study of cell differentiation.
Table of Contents
List of Contributors
Preface
Introduction
Characteristics of Differentiated Cells
Neuroglia—From the Subventricular Precursor to the Mature Cell
Biochemistry of Glial Cells
Physiological and Pharmacological Studies on Central Synaptic Transmission
Characteristics of Neurons in Tissue Culture
Differentiation of Cells in Primary Cultures
Differentiation of Cells in Organ Cultures
Differentiation of Cells in Primary Cultures: Myelination
Synapse Formation in Neural Tissue Cultures
Dissociated Brain Cells in Primary Cultures
Tracing Glial Cell Lineages by Colony Formation in Primary Cultures
Differentiation of Glioblasts under the Influence of Glia Maturation Factor
Neural Cell Isolation and Identification
Cell Lines and Cell Strains
Primary Cultures, Cell Lines, and Cell Strains: Terminology and Characteristics
Glial Cell Lines
Neuronal Cell Lines
Phenotypic Cell Expression
Genetic Control of Neural Development
Specific Protein Species Expressed by Differentiating Neuroblastoma, Glioma, and Hybrid Cells in Culture. Evidence for Neuroblast-Glioblast Reciprocal Genetic Regulation
Immunochemical Markers in Primary Cultures and in Cell Lines
Transmitters, Putative Transmitters, and Transmitter-Related Enzymes Studied in Cultured Cell Systems
Differences between Astrocytes in Primary Cultures and Glial Cell Lines in Uptake and Metabolism of Putative Amino Acid Transmitters
Role of Cyclic Nucleotide in the Differentiation of Nerve Cells
Hormonal Effects on Differentiation in Neural Cultures
Cell Interactions
Cell Aggregation and Recognition in the Self-Assembly of Brain Tissues
Organization of Pathways in Cultures of Mouse Cerebellum
Cell and Organotypic Culture Studies of Neurological Mutations Affecting Structural Development
Neurosecretion in Cultured Systems
A Putative Precursor to Neurophysins and Neurohypophyseal Hormones
Neuroblast-Glioblast Interactions: Ecto-Enzymes
Nutrition
Hormones and Growth Factors in Cell Cultures: Problems and Perspectives
Nutritional Requirements of Cells in Culture, with Special Reference to Neural Cells
Use of Cell Tissue and Organ Cultures in Neurobiology
Cell and Tissue Differentiation in Cultures: Discussion
Cultured Neural Cells and Tissues as Models of Their In Vivo Counterparts: Discussion
Index
Product details
- No. of pages: 708
- Language: English
- Copyright: © Academic Press 1977
- Published: January 1, 1977
- Imprint: Academic Press
- eBook ISBN: 9780323143158
About the Editor
S. Fedoroff
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