Cell, Tissue, and Organ Cultures in Neurobiology

Cell, Tissue, and Organ Cultures in Neurobiology

1st Edition - January 1, 1977

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  • Editor: S. Fedoroff
  • eBook ISBN: 9780323143158

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Description

Cell, Tissue, and Organ Cultures in Neurobiology emerged from an international workshop held at the University of Saskatchewan in March 1977. This book reviews the uses of cell, tissue, and organ cultures in neurobiological research. It brings together an interdisciplinary perspective from morphology, biochemistry, pharmacology, endocrinology, embryology, and genetics. The book is organized into seven parts. Part I contains papers on the characteristics of differentiated cells. Part II presents studies on cell differentiation in primary cultures. Part III deals with studies on cell cultures and cell strains. Part IV focuses on phenotypic cell expression. Part V examines various cellular interactions. Part VI covers studies on nutrition while Part VII takes up applications of cell tissue and organ cultures in neurobiology. The book is directed toward tissue culturists concerned with the nervous system, as well as all neurobiologists, cell biologists, and embryologists interested in learning how neural cells and tissues behave in cultures and what has been learned about the nervous system using tissue culture methods, including the applicability of tissue cultures to the study of cell differentiation.

Table of Contents


  • List of Contributors

    Preface

    Introduction

    Characteristics of Differentiated Cells

    Neuroglia—From the Subventricular Precursor to the Mature Cell

    Biochemistry of Glial Cells

    Physiological and Pharmacological Studies on Central Synaptic Transmission

    Characteristics of Neurons in Tissue Culture

    Differentiation of Cells in Primary Cultures

    Differentiation of Cells in Organ Cultures

    Differentiation of Cells in Primary Cultures: Myelination

    Synapse Formation in Neural Tissue Cultures

    Dissociated Brain Cells in Primary Cultures

    Tracing Glial Cell Lineages by Colony Formation in Primary Cultures

    Differentiation of Glioblasts under the Influence of Glia Maturation Factor

    Neural Cell Isolation and Identification

    Cell Lines and Cell Strains

    Primary Cultures, Cell Lines, and Cell Strains: Terminology and Characteristics

    Glial Cell Lines

    Neuronal Cell Lines

    Phenotypic Cell Expression

    Genetic Control of Neural Development

    Specific Protein Species Expressed by Differentiating Neuroblastoma, Glioma, and Hybrid Cells in Culture. Evidence for Neuroblast-Glioblast Reciprocal Genetic Regulation

    Immunochemical Markers in Primary Cultures and in Cell Lines

    Transmitters, Putative Transmitters, and Transmitter-Related Enzymes Studied in Cultured Cell Systems

    Differences between Astrocytes in Primary Cultures and Glial Cell Lines in Uptake and Metabolism of Putative Amino Acid Transmitters

    Role of Cyclic Nucleotide in the Differentiation of Nerve Cells

    Hormonal Effects on Differentiation in Neural Cultures

    Cell Interactions

    Cell Aggregation and Recognition in the Self-Assembly of Brain Tissues

    Organization of Pathways in Cultures of Mouse Cerebellum

    Cell and Organotypic Culture Studies of Neurological Mutations Affecting Structural Development

    Neurosecretion in Cultured Systems

    A Putative Precursor to Neurophysins and Neurohypophyseal Hormones

    Neuroblast-Glioblast Interactions: Ecto-Enzymes

    Nutrition

    Hormones and Growth Factors in Cell Cultures: Problems and Perspectives

    Nutritional Requirements of Cells in Culture, with Special Reference to Neural Cells

    Use of Cell Tissue and Organ Cultures in Neurobiology

    Cell and Tissue Differentiation in Cultures: Discussion

    Cultured Neural Cells and Tissues as Models of Their In Vivo Counterparts: Discussion

    Index


Product details

  • No. of pages: 708
  • Language: English
  • Copyright: © Academic Press 1977
  • Published: January 1, 1977
  • Imprint: Academic Press
  • eBook ISBN: 9780323143158

About the Editor

S. Fedoroff

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