Cell Separation
Methods and Selected Applications
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Cell Separation: Methods and Selected Applications, Volume 5 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including endothelial cells, separation of lymphoid cells, separation of T lymphocytes, and methods of epidermal separation. Organized into 16 chapters, this volume begins with an overview of the role of endothelium in wound healing and neovascularization as well as in the pathogenesis of many disease states. This text then examines a method of cell separation, isokinetic sedimentation, which can be employed to concentrate and purify one type of cell from a single-cell suspension of disaggregated tissues or organs. Other chapters consider the nature of tumor cell heterogeneity. This book discusses as well the cellular properties essential in malignant tumor behavior. The final chapter deals with transplantable pancreatic acinar cell carcinoma. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.
Table of Contents
Preface
Contents of Previous Volumes
1. Isolation and Characterization of Human Vascular Endothelial Cells with Application to Studies of the Subendothelial Matrix
I. Introduction
II. Isolation of Endothelial Cells
III. Isolation and Characterization of the Subendothelial Matrix
IV. Concluding Remarks
References
2. Separation and Characterization of Lymphoid Cells from Mouse Mammary Carcinomas by Isokinetic Sedimentation
I. Introduction
II. Separation of Lymphoid Cells from Mouse Mammary Tumors
III. Comparison of Results with Isokinetic Sedimentation and Centrifugal Elutriation
IV. Conclusion
References
3. Separation of Malignant Cells from Host Cells Using Centrifugal Elutriation
I. Introduction
II. Theoretical and Practical Aspects
III. Isolation of Host Cells from Tumor Cells
IV. Isolation of Cell Subpopulations from Neoplastic Cells
V. Conclusions
References
4. Selection of Tumor Cell Metastatic Variants by Differential Adhesion to Immobilized Lectins
I. Introduction
II. Experimental Methods
III. Fractionation of Murine Large-Cell Lymphoma Cells
IV. Discussion of the Technique
References
5. Purification of Antigen-Specific Β Cells by Adherence to Whole-Cell Antigens
I. Introduction
II. Methods
III. Assessment of the Technique
IV. Comments on the Technique
References
6. Separation and Characterization of Subpopulations of Τ Cells
I. Introduction
II. Physiochemical Characteristics of Τ Lymphocytes
III. Surface Markers of Τ Lymphocytes
IV. Separation of Τ Lymphocyte Subpopulations
V. Concluding Remarks
References
7. Separation of Pancreatic Islet Cells according to Functional Characteristics
I. Introduction
II. Isolation of Pancreatic Islets
III. Preparation of Islet Cells
IV. Autofluorescence-Activated Sorting of Islet Cells
V. Applicability to Other Cells
VI. Summary
References
8. Separation and Characterization of Leukocytes from the Intestine
I. Introduction
II. Distribution of Lymphoid Cells within the Intestine
III. Isolation of Cells from Peyer's Patches
IV. Isolation of Intraepithelial Leukocytes (IEL)
V. Isolation of Cells from the Lamina Propria
VI. Problems Associated with Isolation
VII. Properties of Intestinal Leukocytes
VIII. Concluding Remarks
References
9. Separation of Epithelial Cells from Gastric and Intestinal Mucosa
I. Introduction
II. Techniques for Isolating Gastric and Intestinal Mucosal Cells
III. Techniques for Cell Separation
IV. Culture of Dispersed Gastrointestinal Epithelial Cells
V. Morphology of Isolated Cells
VI. Functional Characterization of Isolated Gastrointestinal Cells
VII. Concluding Remarks
References
10. Separation of Cells from Normal and Abnormal Epidermis
I. Introduction
II. Methods of Epidermal Separation
III. Methods of Epidermal Cell Separation
IV. Biology and Pathobiology of Epidermal Cell Subpopulations
V. Conclusion
References
11. Isokinetic Gradient Sedimentation of Hematopoietic Progenitor Cells
I. Introduction
II. Preparation of Bone Marrow Cells and Gradients
III. Isokinetic Gradient Sedimentation of Mouse Bone Marrow Cells
IV. Isokinetic Gradient Sedimentation of Human Bone Marrow Cells
V. Concluding Remarks
References
12. Purification of Osteoblasts
I. Introduction
II. Separation of Osteoblasts
III. Purification Methods
IV. Problems of Separated Cells
V. Identification of Osteoblast
VI. Purification of Osteoblasts by FACS
VII. Applications
References
13. Isolation and Separation of Human Colostra! and Milk Cells
I. Introduction
II. Whole-Cell Populations
III. Adherence
IV. Isopycnic Centrifugation
V. Velocity Sedimentation and Isokinetic Centrifugation
VI. Evaluation of Various Techniques
References
14. Isokinetic Gradients for the Separation of Cells
I. Introduction
II. Background
III. Isokinetic Sedimentation
IV. Evaluation of Data
V. Concluding Remarks
References
15. Analysis and Sorting by Flow Cytometry: Applications to the Study of Human Disease
I. Introduction
II. Technical Aspects of Flow Cytometry
III. Sorting Applications in the Study of Human Disease
IV. Appendix: Practical Aspects of Flow Sorting
References
16. Isolation of Granule-Rich and Granule-Deficient Cell Populations from Acinar Carcinoma of Rat Pancreas
I. Transplantable Pancreatic Acinar Carcinoma
II. Isolation of Granule-Rich and Granule-Deficient Carcinoma Cell Populations
III. Perspectives
References
Index
Product details
- No. of pages: 392
- Language: English
- Copyright: © Academic Press 1987
- Published: December 2, 1987
- Imprint: Academic Press
- eBook ISBN: 9781483219424