Cell Separation

Cell Separation

Methods and Selected Applications

1st Edition - December 2, 1987

Write a review

  • Editors: Thomas G. Pretlow, Theresa P. Pretlow
  • eBook ISBN: 9781483219424

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Cell Separation: Methods and Selected Applications, Volume 5 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including endothelial cells, separation of lymphoid cells, separation of T lymphocytes, and methods of epidermal separation. Organized into 16 chapters, this volume begins with an overview of the role of endothelium in wound healing and neovascularization as well as in the pathogenesis of many disease states. This text then examines a method of cell separation, isokinetic sedimentation, which can be employed to concentrate and purify one type of cell from a single-cell suspension of disaggregated tissues or organs. Other chapters consider the nature of tumor cell heterogeneity. This book discusses as well the cellular properties essential in malignant tumor behavior. The final chapter deals with transplantable pancreatic acinar cell carcinoma. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Table of Contents

  • Preface

    Contents of Previous Volumes

    1. Isolation and Characterization of Human Vascular Endothelial Cells with Application to Studies of the Subendothelial Matrix

    I. Introduction

    II. Isolation of Endothelial Cells

    III. Isolation and Characterization of the Subendothelial Matrix

    IV. Concluding Remarks


    2. Separation and Characterization of Lymphoid Cells from Mouse Mammary Carcinomas by Isokinetic Sedimentation

    I. Introduction

    II. Separation of Lymphoid Cells from Mouse Mammary Tumors

    III. Comparison of Results with Isokinetic Sedimentation and Centrifugal Elutriation

    IV. Conclusion


    3. Separation of Malignant Cells from Host Cells Using Centrifugal Elutriation

    I. Introduction

    II. Theoretical and Practical Aspects

    III. Isolation of Host Cells from Tumor Cells

    IV. Isolation of Cell Subpopulations from Neoplastic Cells

    V. Conclusions


    4. Selection of Tumor Cell Metastatic Variants by Differential Adhesion to Immobilized Lectins

    I. Introduction

    II. Experimental Methods

    III. Fractionation of Murine Large-Cell Lymphoma Cells

    IV. Discussion of the Technique


    5. Purification of Antigen-Specific Β Cells by Adherence to Whole-Cell Antigens

    I. Introduction

    II. Methods

    III. Assessment of the Technique

    IV. Comments on the Technique


    6. Separation and Characterization of Subpopulations of Τ Cells

    I. Introduction

    II. Physiochemical Characteristics of Τ Lymphocytes

    III. Surface Markers of Τ Lymphocytes

    IV. Separation of Τ Lymphocyte Subpopulations

    V. Concluding Remarks


    7. Separation of Pancreatic Islet Cells according to Functional Characteristics

    I. Introduction

    II. Isolation of Pancreatic Islets

    III. Preparation of Islet Cells

    IV. Autofluorescence-Activated Sorting of Islet Cells

    V. Applicability to Other Cells

    VI. Summary


    8. Separation and Characterization of Leukocytes from the Intestine

    I. Introduction

    II. Distribution of Lymphoid Cells within the Intestine

    III. Isolation of Cells from Peyer's Patches

    IV. Isolation of Intraepithelial Leukocytes (IEL)

    V. Isolation of Cells from the Lamina Propria

    VI. Problems Associated with Isolation

    VII. Properties of Intestinal Leukocytes

    VIII. Concluding Remarks


    9. Separation of Epithelial Cells from Gastric and Intestinal Mucosa

    I. Introduction

    II. Techniques for Isolating Gastric and Intestinal Mucosal Cells

    III. Techniques for Cell Separation

    IV. Culture of Dispersed Gastrointestinal Epithelial Cells

    V. Morphology of Isolated Cells

    VI. Functional Characterization of Isolated Gastrointestinal Cells

    VII. Concluding Remarks


    10. Separation of Cells from Normal and Abnormal Epidermis

    I. Introduction

    II. Methods of Epidermal Separation

    III. Methods of Epidermal Cell Separation

    IV. Biology and Pathobiology of Epidermal Cell Subpopulations

    V. Conclusion


    11. Isokinetic Gradient Sedimentation of Hematopoietic Progenitor Cells

    I. Introduction

    II. Preparation of Bone Marrow Cells and Gradients

    III. Isokinetic Gradient Sedimentation of Mouse Bone Marrow Cells

    IV. Isokinetic Gradient Sedimentation of Human Bone Marrow Cells

    V. Concluding Remarks


    12. Purification of Osteoblasts

    I. Introduction

    II. Separation of Osteoblasts

    III. Purification Methods

    IV. Problems of Separated Cells

    V. Identification of Osteoblast

    VI. Purification of Osteoblasts by FACS

    VII. Applications


    13. Isolation and Separation of Human Colostra! and Milk Cells

    I. Introduction

    II. Whole-Cell Populations

    III. Adherence

    IV. Isopycnic Centrifugation

    V. Velocity Sedimentation and Isokinetic Centrifugation

    VI. Evaluation of Various Techniques


    14. Isokinetic Gradients for the Separation of Cells

    I. Introduction

    II. Background

    III. Isokinetic Sedimentation

    IV. Evaluation of Data

    V. Concluding Remarks


    15. Analysis and Sorting by Flow Cytometry: Applications to the Study of Human Disease

    I. Introduction

    II. Technical Aspects of Flow Cytometry

    III. Sorting Applications in the Study of Human Disease

    IV. Appendix: Practical Aspects of Flow Sorting


    16. Isolation of Granule-Rich and Granule-Deficient Cell Populations from Acinar Carcinoma of Rat Pancreas

    I. Transplantable Pancreatic Acinar Carcinoma

    II. Isolation of Granule-Rich and Granule-Deficient Carcinoma Cell Populations

    III. Perspectives



Product details

  • No. of pages: 392
  • Language: English
  • Copyright: © Academic Press 1987
  • Published: December 2, 1987
  • Imprint: Academic Press
  • eBook ISBN: 9781483219424

About the Editors

Thomas G. Pretlow

Theresa P. Pretlow

Ratings and Reviews

Write a review

There are currently no reviews for "Cell Separation"