Cell Separation

Methods and Selected Applications

1st Edition - December 2, 1987
Editors: Thomas G. Pretlow, Theresa P. Pretlow
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 1 9 4 2 - 4

Cell Separation: Methods and Selected Applications, Volume 5 provides information pertinent to the design and application of methods for the separation of cells. This book covers a… Read more

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Cell Separation: Methods and Selected Applications, Volume 5 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including endothelial cells, separation of lymphoid cells, separation of T lymphocytes, and methods of epidermal separation. Organized into 16 chapters, this volume begins with an overview of the role of endothelium in wound healing and neovascularization as well as in the pathogenesis of many disease states. This text then examines a method of cell separation, isokinetic sedimentation, which can be employed to concentrate and purify one type of cell from a single-cell suspension of disaggregated tissues or organs. Other chapters consider the nature of tumor cell heterogeneity. This book discusses as well the cellular properties essential in malignant tumor behavior. The final chapter deals with transplantable pancreatic acinar cell carcinoma. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Preface

Contents of Previous Volumes

1. Isolation and Characterization of Human Vascular Endothelial Cells with Application to Studies of the Subendothelial Matrix

I. Introduction

II. Isolation of Endothelial Cells

III. Isolation and Characterization of the Subendothelial Matrix

IV. Concluding Remarks

References

2. Separation and Characterization of Lymphoid Cells from Mouse Mammary Carcinomas by Isokinetic Sedimentation

I. Introduction

II. Separation of Lymphoid Cells from Mouse Mammary Tumors

III. Comparison of Results with Isokinetic Sedimentation and Centrifugal Elutriation

IV. Conclusion

References

3. Separation of Malignant Cells from Host Cells Using Centrifugal Elutriation

I. Introduction

II. Theoretical and Practical Aspects

III. Isolation of Host Cells from Tumor Cells

IV. Isolation of Cell Subpopulations from Neoplastic Cells

V. Conclusions

References

4. Selection of Tumor Cell Metastatic Variants by Differential Adhesion to Immobilized Lectins

I. Introduction

II. Experimental Methods

III. Fractionation of Murine Large-Cell Lymphoma Cells

IV. Discussion of the Technique

References

5. Purification of Antigen-Specific Β Cells by Adherence to Whole-Cell Antigens

I. Introduction

II. Methods

III. Assessment of the Technique

IV. Comments on the Technique

References

6. Separation and Characterization of Subpopulations of Τ Cells

I. Introduction

II. Physiochemical Characteristics of Τ Lymphocytes

III. Surface Markers of Τ Lymphocytes

IV. Separation of Τ Lymphocyte Subpopulations

V. Concluding Remarks

References

7. Separation of Pancreatic Islet Cells according to Functional Characteristics

I. Introduction

II. Isolation of Pancreatic Islets

III. Preparation of Islet Cells

IV. Autofluorescence-Activated Sorting of Islet Cells

V. Applicability to Other Cells

VI. Summary

References

8. Separation and Characterization of Leukocytes from the Intestine

I. Introduction

II. Distribution of Lymphoid Cells within the Intestine

III. Isolation of Cells from Peyer's Patches

IV. Isolation of Intraepithelial Leukocytes (IEL)

V. Isolation of Cells from the Lamina Propria

VI. Problems Associated with Isolation

VII. Properties of Intestinal Leukocytes

VIII. Concluding Remarks

References

9. Separation of Epithelial Cells from Gastric and Intestinal Mucosa

I. Introduction

II. Techniques for Isolating Gastric and Intestinal Mucosal Cells

III. Techniques for Cell Separation

IV. Culture of Dispersed Gastrointestinal Epithelial Cells

V. Morphology of Isolated Cells

VI. Functional Characterization of Isolated Gastrointestinal Cells

VII. Concluding Remarks

References

10. Separation of Cells from Normal and Abnormal Epidermis

I. Introduction

II. Methods of Epidermal Separation

III. Methods of Epidermal Cell Separation

IV. Biology and Pathobiology of Epidermal Cell Subpopulations

V. Conclusion

References

11. Isokinetic Gradient Sedimentation of Hematopoietic Progenitor Cells

I. Introduction

II. Preparation of Bone Marrow Cells and Gradients

III. Isokinetic Gradient Sedimentation of Mouse Bone Marrow Cells

IV. Isokinetic Gradient Sedimentation of Human Bone Marrow Cells

V. Concluding Remarks

References

12. Purification of Osteoblasts

I. Introduction

II. Separation of Osteoblasts

III. Purification Methods

IV. Problems of Separated Cells

V. Identification of Osteoblast

VI. Purification of Osteoblasts by FACS

VII. Applications

References

13. Isolation and Separation of Human Colostra! and Milk Cells

I. Introduction

II. Whole-Cell Populations

III. Adherence

IV. Isopycnic Centrifugation

V. Velocity Sedimentation and Isokinetic Centrifugation

VI. Evaluation of Various Techniques

References

14. Isokinetic Gradients for the Separation of Cells

I. Introduction

II. Background

III. Isokinetic Sedimentation

IV. Evaluation of Data

V. Concluding Remarks

References

15. Analysis and Sorting by Flow Cytometry: Applications to the Study of Human Disease

I. Introduction

II. Technical Aspects of Flow Cytometry

III. Sorting Applications in the Study of Human Disease

IV. Appendix: Practical Aspects of Flow Sorting

References

16. Isolation of Granule-Rich and Granule-Deficient Cell Populations from Acinar Carcinoma of Rat Pancreas

I. Transplantable Pancreatic Acinar Carcinoma

II. Isolation of Granule-Rich and Granule-Deficient Carcinoma Cell Populations

III. Perspectives

References

Index