Cell Separation

Cell Separation

Methods and Selected Applications

1st Edition - June 5, 1984

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  • Editors: Thomas G. Pretlow, Theresa P. Pretlow
  • eBook ISBN: 9781483219400

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Cell Separation: Methods and Selected Application, Volume 3 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including lymphoma cells, lectins, purification of cells from lung tumors, macrophage electrophoretic migration test, tissue heterogeneity, and characteristics of cultured cells. Organized into 13 chapters, this volume begins with an overview of the approaches in examining particular cell-surface properties and their role in the metastatic process. This text then examines lectins as important tools for identification and separation of cells, particularly of lymphocyte subpopulations. Other chapters consider the various methods that have been used to disperse rat pituitary tissue into single cells. This book discusses as well the different methods for isolating type II cells. The final chapter deals with the significance of having cell cultures homogeneous for a given cell type. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Table of Contents

  • Contributors


    Contents of Previous Volumes

    1. Separation of Malignant Lymphoid Cells by Countercurrent Distribution

    I. Introduction

    II. Experimental Methods

    III. Fractionation of RAW117 Lymphoma Cells

    IV. Discussion of the Technique


    2. Use of Lectins for Separation of Cells

    I. Introduction

    II. Methodology

    III. Selected Applications

    IV. Concluding Remarks


    3. Enzymatic Disaggregation of Human Bronchogenic Carcinomas followed by Velocity Sedimentation of Cells

    I. Introduction

    II. Enzymatic Disaggregation of Tumors

    III. Suspensions of Tumors: Storage and Composition

    IV. Purification of Cells from Lung Tumors

    V. Concluding Remarks


    4. Macrophage Electrophoretic Migration (MEM) Test with Some Illustrative and Heuristic Applications

    I. Historical Introduction

    II. Basic Requirements

    III. Preparation of Macrophages

    IV. Preparation of Lymphocytes

    V. Procedure: Cancer

    VI. Applications


    5. Implementation of Microtechniques to Resolve Tissue Heterogeneity

    I. Introduction

    II. Description of Microtechniques

    III. Cell Differentiation in the Cellular Slime Mold Dictyostelium discoideum

    IV. Adaptation of the Micromethods to Human Mammary Tumors

    V. Conclusions


    6. In Vitro Isolation of Malignant Cells from Small Cell Carcinomas

    I. Introduction

    II. Establishment of Cell Lines

    III. Characteristics of Cultured Cells

    IV. Summary


    7. Computer-Controlled Cell Electrophoresis Microscope

    I. Introduction

    II. Design of the Automated Electrophoresis Microscope System (AEMS)

    III. Features Important to the Operation of the AEMS

    IV. Conclusions


    8. Separation of Cells from the Rat Anterior Pituitary Gland

    I. Cellular Organization of the Rat Anterior Pituitary Gland

    II. Preparation of Rat Pituitary Cell Suspensions

    III. Methods Used to Differentiate Pituitary Cell Types

    IV. Review of Pituitary Cell Separation Studies

    V. Separated Pituitary Cells: Applications

    VI. Concluding Remarks


    9. Isolation of Type II Pneumocytes and Their Application in the Study of Pulmonary Metabolism

    I. Introduction

    II. Overview—Type II Cell Isolation

    III. Method of Isolation

    IV. Applications

    V. Conclusion


    10. Selective Media

    I. Introduction

    II. Biological Basis for Selective Growth

    III. Families of Cells with Similar Growth Requirements

    IV. Selective Growth of Human Epidermal Keratinocytes

    V. Techniques for Achieving Selective Growth

    VI. Future Prospects


    11. Cell Labeling and Separation Using Immunospecific Microspheres

    I. Introduction

    II. General Considerations

    III. Cell-Specific Ligands

    IV. Microspheres for Cell Labeling and Separation

    V. Cell-Labeling Methods

    VI. Applications of Microspheres in Cell Separations

    VII. Summary and Concluding Remarks


    12. Collagenase and the Isolation of Cells from Bone

    I. Cells of the Osteoblast Line

    II. Cells of the Osteoclast Line

    III. Isolation of Calvarial Cells

    IV. Clostridium histolyticum Collagenase

    V. Cell Separation Techniques

    VI. Culture of Human Bone Cells

    VII. Freezing Cells for Storage


    13. Cell Separation by Biological Methods

    I. Introduction

    II. Differential Culture Procedures

    III. Selective Reagents

    IV. Selective Media

    V. Discussion



Product details

  • No. of pages: 336
  • Language: English
  • Copyright: © Academic Press 1984
  • Published: June 5, 1984
  • Imprint: Academic Press
  • eBook ISBN: 9781483219400

About the Editors

Thomas G. Pretlow

Theresa P. Pretlow

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