Cell Separation

Cell Separation

Methods and Selected Applications

1st Edition - September 28, 1983

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  • Editors: Thomas G. Pretlow, Theresa P. Pretlow
  • eBook ISBN: 9781483219394

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Cell Separation: Methods and Selected Applications, Volume 2 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including thyroid structure and function, procurement of eosinophils, elutriation, stromal cells, isolation of epithelial cells, and separation of cells. Organized into 15 chapters, this volume begins with an overview of the structure of thyroid parenchyma and the properties of endocrine parafollicular cells. This text then summarizes the various aspects of the separation methodology to assist investigators with the use of elutriation. Other chapters describe the methods that are useful for the separation of stromal cells from tumors. This book discusses as well the types of methods that are available for the purification of cells from tumors. The final chapter deals with the biological significance of the erythroblastic nest. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Table of Contents

  • Contributors

    Contents of Volume 1


    1. Separation of Parafollicular Cells from Thyroid Follicular Cells by Affinity Chromatography Using Thyroglobulin-Coupled Sepharose

    I. Thyroid Structure and Function

    II. Parafollicular Cells and Serotonin

    III. Obtaining Parafollicular Cells for Study

    IV. Serotonin Binding Protein

    V. Distribution of Parafollicular Cells

    VI. Dissociation of the Cells of the Thyroid

    VII. Separation of Parafollicular Cells


    2. Procurement and Purification of Eosinophils

    I. Introduction

    II. Procurement of Eosinophils

    III. Purification

    IV. Summary and Conclusions


    3. Experimental Factors Involved in Separation by Centrifugal Elutriation

    I. Introduction

    II. Methodology of Elutriation

    III. Concluding Remarks


    4. Analysis and Separation of Stromal Cells Infiltrating Tumors

    I. Introduction

    II. Previous Work

    III. Quantitation and Subcompartments

    IV. Identification of Stromal Cells and Morphometry

    V. Purification of Cells from Tumors

    VI. Concluding Comments


    5. Selective Isolation of Epithelial Cells in Primary Expiant Cultures of Human and Animal Tissues

    I. Introduction

    II. Initiation of Primary Expiant Cultures

    III. Selective Isolation of Epithelial Cells

    IV. Identification of Human Bronchial and Bladder Epithelial Cells in Primary Expiant Cultures

    V. Discussion

    VI. Other Techniques


    6. Isolation of Human Natural Killer Cells (Large Granular Lymphocytes)

    I. Introduction

    II. Isolation of Large Granular Lymphocytes (LGL)

    III. Comments


    7. Purification of Islets and Cells from Islets

    I. Introduction

    II. Isolation of Pancreatic Islets

    III. Islet Purification

    IV. Preparation of Islet Cells

    V. Separation of Islet Cell Subpopulations

    VI. Summary


    8. Magnetic Cell Sorting

    I. Introduction

    II. Magnetism and Magnetic Forces

    III. High Gradient Magnetic Separation

    IV. Magnetic Particles for Cell Labeling

    V. Conclusions


    9. Separation of Cells by Preparative Density Gradient Electrophoresis

    I. Introduction

    II. Electrophoresis Theory

    III. Preparative Cell Electrophoresis in Density Gradients

    IV. Separation and Characterization of Cells by Density Gradient Electrophoresis

    V. Concluding Remarks


    10. Isolation, Characterization, and Culture of Human Mammary Epithelial Cells

    I. Introduction

    II. Isolation of Mammary Epithelial Cells in Culture

    III. Method for the Culture of Human Mammary Epithelial Cells

    IV. Verification of the Mammary Epithelial Origin of the Cultured Cells

    V. Verification of the Malignant Origin of the Epithelial Cells Derived from Tumor Specimens

    VI. A Marker of Early Stages in Malignant Progression

    VII. Use of the Clonal Assay to Study Chemotherapeutic Drug Sensitivity

    VIII. Summary


    11. Solid-Phase Fractionation of Lymphoid Cells on Ligand-Coated Plastic Plates

    I. Introduction

    II. Lymphocyte Fractionation

    III. Isolation of Surface Immunoglobulin-Positive Lymphocytes

    IV. Isolation of Human Fc Receptor-Bearing Lymphocytes on Antigen-Antibody Coated Dishes

    V. Isolation of Fc Receptor-Bearing Human T Lymphocytes

    VI. Isolation of Antigen-Specific Lymphocytes

    VII. Isolation of Lymphocyte Subsets with Monoclonal Antibodies

    VIII. Summary


    12. Large Capacity Separation of Cells in a Reorienting Zonal Rotor

    I. Introduction

    II. General Characteristics of Reorienting Zonal Rotors

    III. Types of Separations Carried out in the TX-28 Rotor

    IV. Choice of Reorienting Zonal Rotor Versus Other Techniques

    V. Concluding Comments


    13. Short-Term Culture of Cells from Human Solid Tumors in Semisolid Medium

    I. Introduction

    II. Methodology for Clonogenic Assays

    III. Conclusions


    14. Preparation and Performance of the Erythrocyte-Unsaturated Fatty Acid (E-UFA) Test

    I. Historical Introduction

    II. Technique of Red Blood Cell (RBC) Preparation

    III. Measurement of Absolute Mobility

    IV. Precautions: Interfering Effects of Medication

    V. Some Unexpected Results

    VI. "Silent" and Subclinical Multiple Sclerosis

    VII. Etiology and Pathogenesis of Multiple Sclerosis

    VIII. Summary


    15. Isolation of Erythroblastic Nests and Separation of Their Cellular Components

    I. Introduction

    II. Definitions

    III. Why Erythroblastic Nest (EN)?

    IV. Method and Guidelines

    V. Generation of Erythropoietic Spleens Containing ENs

    VI. Preparation of Cell Suspensions from Erythropoietic Spleens

    VII. Preparation of Spleen Cell Suspensions Enriched in ENs

    VIII. EN Purification

    IX. EN Dissociation

    X. Separation of EN Central Cells from Erythroblasts

    XI. Summation of Methods and Selected Applications

    XII. Conclusions

    XIII. Perspectives for the Future



Product details

  • No. of pages: 344
  • Language: English
  • Copyright: © Academic Press 1983
  • Published: September 28, 1983
  • Imprint: Academic Press
  • eBook ISBN: 9781483219394

About the Editors

Thomas G. Pretlow

Theresa P. Pretlow

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