Cell Separation

Cell Separation

Methods and Selected Applications

1st Edition - December 28, 1982

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  • Editors: Thomas G. Pretlow, Theresa P. Pretlow
  • eBook ISBN: 9781483219387

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Cell Separation: Methods and Selected Applications is a compendium of articles on the design and/or application of methods for the separation of cells. This volume presents contributions on relatively finite subjects on cell separation. It covers topics on cell separation such as methods for obtaining cells in suspension from animal tissues; some of the kinds of data that are helpful in the description of cell purifications; and separation of host cells infiltrating tumors and allografts by velocity sedimentation at unit gravity. The separation of different kinds of nucleated cells from blood by centrifugal elutriation; a new approach to the separation of cells at unit gravity; and the isolation and culture of homogeneous populations of glomerular cell types are elucidated as well. Experimental oncologists, hematologists, immunologists, cell biologists, endocrinologists, and others who are not already expert in the use of methods for cell separation will find the book highly useful.

Table of Contents

  • Contributors


    1. Methods for Obtaining Cells in Suspension from Animal Tissues

    I. Introduction

    II. Nonenzymatic Methods of Tissue Disaggregation

    III. Proteolytic Enzymes for Disaggregation

    IV. Subsidiary Enzymes

    V. Protease Inhibitors

    VI. Protective Agents

    VII. Concluding Remarks


    2. Evaluation of Data, Problems, and General Approach

    I. Introduction

    II. Markers

    III. Quantitative Characterization of Cells Before and After Cell Separation

    IV. Morphological Criteria

    V. Concluding Comments


    3. Sedimentation of Cells: An Overview and Discussion of Artifacts

    I. Introduction

    II. Theory

    III. Velocity Sedimentation

    IV. Isopycnic Sedimentation

    V. Comparison of Velocity and Isopycnic Sedimentation

    VI. Differential Sedimentation and Discontinuous Gradients

    VII. Artifacts in Sedimentation of Cells

    VIII. Concluding Remarks


    4 . Separation of Host Cells Infiltrating Tumors and Allografts by Velocity Sedimentation at Unit Gravity

    I. Introduction

    II. Theoretical Considerations

    III. Potential Problems and Practical Limitations

    IV. Velocity Sedimentation of Dissociated Animal Tumors

    v. Velocity Sedimentation of Ascites Tumors, Leukemias, and Lymphomas

    VI. Velocity Sedimentation of Disaggregated Human Neoplasms

    VII. Recovery of Allograft-infiltrating Host Cells by 1 g Velocity Sedimentation

    VIII. Concluding Remarks


    5. Analytical Characterization of Adult Granulocyte-Macrophage Progenitor Cells by Sedimentation Velocity and Buoyant Density

    I. Introduction

    II. Purification Studies

    III. Characterization of the Clonable Mouse Granulocyte-Macrophage Progenitor Cells (CFU-C)

    IV. Relationship of Mouse CFU-C to Other Clonable Hemopoietic Precursors and Stern Cells

    V. Physical Properties of Human CIonable Granulocyte and Macrophage Progenitor Cells

    VI. Conclusions


    6. Sedimentation of Cells in Colloidal Silica (Percoll)

    I. Introduction

    II. Properties of Percoll

    III. Principles of Cell Separation in Percoll

    IV. Practical Aspects of the Use of Percoll

    v. Does Percoll Interfere with Cell Functions?

    VI. Future Use of Percoll


    7. Separation of Different Kinds of Nucleated Cells from Blood by Centrifugal Elutriation

    I. Introduction

    II. Physical Characteristics of Human Blood Leukocytes

    III. Practical Technique

    IV. Isolation of Lymphocytes

    V. Isolation of Monocytes

    VI. Isolation of Granulocytes

    VII. Concluding Remarks


    8. A New Approach to the Separation of Cells at Unit Gravity

    I. Introduction

    II. Theory

    III. CelSep Apparatus for Unit Gravity Separations

    IV. Standard Separation Procedure

    V. Separation of Human Monocytes from Blood

    VI. Characterization of Human Myeloid Stem Cells

    VII. Fractionation of Canine Gastric Cells

    VIII. Advantages and Disadvantages of the CelSep Method


    9. Electronic Cell Sorting of Hemopoietic Progenitor Cells

    I. Introduction

    II. General Considerations

    III. Sorting of Unstained Hemopoietic Cells

    IV. Sorting of Hemopoietic Cells Labeled with Antibodies

    V. Sorting of Hemopoietic Cells Labeled with Lectins

    VI. Sorting of Hemopoietic Cells by Other Techniques

    VII. Summary and Future Perspectives


    10. Separation of Individual Cells from the Fundic Gastric Mucosa

    I. Introduction

    II. Methods for Gastric Mucosal Cell Isolation

    III. Methods for Cell Purification

    IV. General Characteristics of Isolated Gastric Cells

    V. Specific Advantages and Disadvantages of the Various Purification Methods

    VI. Functional Characterization of Isolated Gastric Cells


    11. Isolation and Culture of Homogeneous Populations of Glomerular Cell Types

    I. Introduction

    II. Background

    III. Techniques for Isolating Glomerular Cells

    IV. Tissue Culture Medium Used to Culture Glomerular Cells

    V. Properties of Glomerular Cells in Culture

    VI. Future Areas of Research on the Biology and Pathobiology of Glomerular Cells


    12. Separation and Subfractionation of Blood Cell Populations Based on Their Surface Properties by Partitioning in Two-Polymer Aqueous Phase Systems

    I. Introduction

    II. Separation and Subfractionation of Blood Cells

    III. Cell-Cell Affinity Reflected by Countercurrent Distribution

    IV. Conclusion


    13. Purification of Basophilic Leukocytes from Guinea Pig and Human Blood and from Guinea Pig Bone Marrow

    I. Introduction

    II. Purification of Guinea Pig Blood and Bone Marrow Basophils

    III. Purification of Human Peripheral Blood Basophils



Product details

  • No. of pages: 342
  • Language: English
  • Copyright: © Academic Press 1982
  • Published: December 28, 1982
  • Imprint: Academic Press
  • eBook ISBN: 9781483219387

About the Editors

Thomas G. Pretlow

Theresa P. Pretlow

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