Cell of Immunoglobulin Synthesis

Cell of Immunoglobulin Synthesis

1st Edition - January 1, 1979

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  • Editor: Benvenuto Pernis
  • eBook ISBN: 9780323149686

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Description

Cells of Immunoglobulin Synthesis documents the proceedings of a symposium on ""Cells of Immunoglobulin Synthesis"" held at Arden House, on the Harriman Campus of Columbia University from June 9-11, 1978. The meeting was the third of the P & S Biomedical Sciences Symposia. Starting with the opening address on B lymphocyte differentiation and the tolerance problem, the remainder of the book presents the contributions made by researchers at the symposium. These contributions are organized into seven parts. Part I contains studies on immunoglobulin genes, messages, and molecules. Part II presents studies on cellular immunoglobulin production. Part III examines membrane immunoglobulins while Part IV focuses on immunoglobulins as regulatory molecules. Part V deals with lymphocyte hybrids. Part VI examines immunoglobulin idiotypes while Part VII contains papers on the ontogeny of immunoglobulin-synthesizing cells.

Table of Contents


  • List of Participants

    Preface

    Opening Address

    B Lymphocyte Differentiation and the Tolerance Problem

    Sequence of Acquisition of la, IgM, and IgD in Adult Murine Bone Marrow

    Kinetics of Tolerogenesis among Immature B Cells of Various Categories

    The Cambier Dilemma — Differences between T-Independent and T-Dependent Cells

    Multivalency of Tolerogens and the Nature of Tolerance Phenomena in Adult Animals

    Conclusions

    References

    Part I Immunoglobulin Genes, Messages, and Molecules

    Somatic Recombination and Structure of an Immunoglobulin Gene

    Introduction

    Scheme for Isolation of Ig Genes from Cellular DNA

    DNA Clones Isolated from Embryo and Myeloma Cells

    Type of Gene Sequences Contained in the DNA Clones

    Location of the λ Chain Gene Sequence in the DNA Clones

    Sequence Homology between the Cloned DNA Fragments

    Evidence for Somatic Rearrangement of Immunoglobulin Genes

    References

    Implications of the Assortment of Framework Segments for the Assembly of Immunoglobulin VL and VH Regions and the Generation of Diversity

    Text

    References

    Immunoglobulin Genes and Nuclear RNA Precursors

    Introduction

    Results and Discussion

    Closing Remarks

    References

    Studies on mRNA Sequence and Immunoglobulin Gene Organization Using Synthetic Oligonucleotides

    Introduction

    Studies with 32P-mRNA and cDNA Primed with Oligo(dT)

    Studies with Specific Primers

    The Uses of mRNA Nucleotide Sequence Data

    Hybrid Myeloma Cells and the Specificity of V-C Integration

    Summary

    References

    Comparative Aspects of in Vitro and Cellular Assembly of Immunoglobulins

    Text

    References

    Human Immunoglobulin Mutants

    Text

    References

    Part II Cellular Immunoglobulin Production

    Variable and Constant Region Variants of Mouse Myeloma Cells

    Introduction

    Variants with Changes in Antigen Binding

    Constant Region Mutants

    Conclusions

    References

    Constant Region Mutants of Mouse Immunoglobulins

    Introduction

    Isolation and Characterization of 10-1, a Mutant Cell Line Synthesizing an Internally

    Deleted Heavy Chain

    Mutants Derived from the 10-1 Cell Line

    Subclass Switch Mutants from G251 and 117

    Mechanisms of Subclass Switch

    References

    Differentiation of Leukemic B Lymphocytes in Man

    Introduction

    In Vivo Differentiation

    Induction of in Vitro Differentiation of Leukemic B Lymphocytes

    Epstein-Barr Virus Transformation of Leukemic B Lymphocytes

    Defective T Cell Helper Function in Patients

    Discussion and Conclusion

    References

    Intracellular Events in the Differentiation of B Lymphocytes to Pentamer IgM Synthesis

    Text

    References

    Part III Membrane Immunoglobulins

    Receptor-Mediated Triggering and Tolerance in Murine B Cells

    The Role of Surface Igs in B Cell Tolerance

    The Role of Surface Igs in B Cell Triggering

    References

    Structure of Lymphocyte Membrane Immunoglobulin

    Introduction

    Methods

    Results

    Discussion

    References

    Role of Membrane Immunoglobulins in Lymphocyte Responses

    Introduction

    Effect of Anti-Immunoglobulin Antibodies on B Lymphocyte Maturation Induced by LPS

    Effect of Antigen on B Lymphocyte Maturation Induced by LPS

    Summary

    References

    Part IV Immunoglobulins as Regulatory Molecules

    Function of Surface Immunoglobulin of B Lymphocytes

    Capping

    Endocytosis of Ig-Ligand Complexes

    Receptor Reexpression

    Modulation of Proliferation

    References

    The Role of Immunoglobulin in the Induction of B Lymphocytes

    Introduction

    Results

    Discussion

    References

    Role of the Fc Portion of Antibody in Immune Regulation

    Introduction

    Activation of B Lymphocytes by the Fc Portion of Immunoglobulin

    Summary

    References

    Factors Affecting the Triggering of the B-Cell Repertoire

    Introduction

    The Clonotype Repertoire

    B-Cell Subpopulations

    Antigen Form

    Modes of Antigenic Stimulation

    Suppression

    Conclusion

    References

    Part V Lymphocyte Hybrids

    Fusion of Immunoglobulin Secreting Cells

    Introduction

    Hybridomas

    Cellular Restrictions on Hybridoma Fusion

    Molecular Biology of Immunoglobulins

    Basis of Differentiation

    References

    Analysis of the Diversity of Murine Antibodies to α(1→3) Dextran

    Introduction

    Extent of Diversity of Anti-α(1→3) Dextran Repertoire

    Correlation between Spectrotype, Idiotype, and Isotype

    Somatic Cell Hybrids

    Summary

    References

    Monoclonal Antibodies to Alloantigens and to Immunoglobulin Allotypes

    Introduction

    Production of Hybrids

    Antibodies to Alloantigens of the Major Histocompatibility Complex

    Purification and Chain Composition of Hybrid Cell Antibodies

    Antibodies to Immunoglobulin Allotypes

    Conclusions

    References

    Part VI Immunoglobulin Idiotypes

    Idiotype-Specific Suppression Mediated by B Cells

    Introduction

    Results and Discussion

    References

    Regulation of the Immune System by Idiotype-Anti-Idiotype Interaction in the Rabbit and the Mouse

    Introduction

    Idiotypic Manipulation of the Mouse Immune System

    VH Allotypic Background and Network

    Comparison between Idiotype of Ab1 and Idiotype of Ab3

    Conclusion

    References

    Ontogeny of Clonal Dominance

    Introduction

    Loss of Clonal Dominance after Adoptive Transfer

    Rescue of Clonal Dominance

    Discussion

    Summary

    References

    Anti-(T,G)-A--L Idiotypes: Initial Studies of Genetic Control and Cellular Expression

    Introduction

    Production and Specificity of Antisera Directed against Anti-(T,G)-A--L Idiotypes

    Genetic Control of the Expression of BIO Anti-(T,G)-A--L Idiotype(s)

    Cellular Expression of Anti-(T,G)-A--L Idiotype(s)

    References

    Part VII Ontogeny of Immunoglobulin-Synthesizing Cells

    B Lymphocyte Development and Activation: Analysis with a Mutant Mouse Strain

    Introduction

    Conclusion

    References

    Influence of Thymus Cells on the Ontogeny of B Lymphocyte Function

    Introduction

    Methods and Rationale

    Results and Discussion

    Conclusions

    References

    Pre-B Cells: Normal Morphologic and Biologic Characteristics and Abnormal Development in Certain Immunodeficiencies and Malignancies

    Introduction

    Historical Perspective of the Identification of Pre-B Cells

    Ontogeny, Morphology, and Organ Distribution of Pre-B Cells

    Growth Characteristics of Pre-B Cells

    Evidence for Precursor-Progeny Relationship of Pre-B Cells and B Lymphocytes

    Surface and Cytoplasmic Constituents of Pre-B Cells

    Absence of Functional Responses of Pre-B Cells

    Evidence for Clonal Diversity of Pre-B Cells

    Pre-B Cells in Immunodeficiencies

    Malignancies Involving Pre-B Cells

    Conclusion

    References

    Index






Product details

  • No. of pages: 456
  • Language: English
  • Copyright: © Academic Press 1979
  • Published: January 1, 1979
  • Imprint: Academic Press
  • eBook ISBN: 9780323149686

About the Editor

Benvenuto Pernis

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