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David Caballero, Subhas C. Kundu and Rui L. Reis
Section 1: Tissue decellularization and characterization methods
1. Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels
Rabeil Sakina, Aida Llucià-Valldeperas, Ana Henriques Lourenço, Abhishek Harichandan, Sandro Gelsomino, Paul Wieringa, Carlos Mota and Lorenzo Moroni
2. Method to decellularize the human chorion membrane
Laura P. Frazão, Joana Vieira-de-Castro, Cristina Nogueira-Silva and Nuno M. Neves
3. Protocols for decellularization of human amniotic membrane
Sadjad Khosravimelal, Maryam Momeni, Mahdieh Gholipur, Subhas C. Kundu and Mazaher Gholipourmalekabadi
4. Use of supercritical CO2 in soft tissue decellularization
Bengisu Topuz, Gülçin Günal, Selcan Guler and Halil Murat Aydin
5. Corneal extracellular matrix decellularization
Section 2: Decellularized tissues for tissue engineering and disease modelling
6. Implementation of a basement membrane invasion assay using mesenteric tissue
Ritobrata Ghose, Alistair J. Rice, Ernesto Cortes, Upamanyu Ghose, Dariusz Lachowski and Armando del Rio Hernandez
7. Decellularized human bone as a 3D model to study skeletal progenitor cells in a natural environment
Ana Rita Pereira, Maximilian Rudert and Marietta Herrmann
8. Cell sheet tissue engineering for scaffold-free three-dimensional (3D) tissue reconstruction
Kyungsook Kim, Sophia Bou-Ghannam and Teruo Okano
9. Decellularized matrices for tumor cell modeling
Virginia Brancato, Maurizio Ventre, Rui L. Reis and Paolo Antonio Netti
10. Tissue-derived decellularized extracellular matrices toward cartilage repair and regeneration
Piyali Das, Yogendra Pratap Singh, Biman B. Mandal and Samit Kumar Nandi
Section 3: Hybrid systems
11.The effect of aligned electrospun fibers and macromolecular crowding in tenocyte culture
Dimitrios Tsiapalis, Shubhasmin Rana, Meletios Doulgkeroglou, Stephen Kearns, Jack Kelly, Yves Bayon and Dimitrios I. Zeugolis
Cell-Derived Matrices Part B, Volume 157 provides a detailed description and step-by-step methods surrounding the use of three-dimensional cell-derived matrices for tissue engineering applications. Chapters in this new release include Glaucomatous cell-derived matrices, Cardiac tissue explants decellularization, Decellularization of skin matrices for wound healing applications, Guiding axonal growth by aligned cell-derived matrices for spinal cord injury regeneration, Human Mesenchymal Stem Cell–Derived Matrices for Enhanced Osteoregeneration, Amniotic decellularized matrices, Three-Dimensional (3-D) Tissue Reconstruction without Scaffold, Tubular cell-derived matrices for TERM applications, and more.
- Contains contributions from leading experts in the field from across the globe
- Covers a wide array of topics surrounding the use of cell-derived matrices for tissue engineering and regenerative medicine applications
- Includes relevant, analysis-based topics, such as the quantification of mechanical properties, decellularization protocols, and innovative matrix engineering methods
Postgraduates and researchers in the area of tissue engineering, biophysics and cellular biology, and other related scientific areas
- No. of pages:
- © Academic Press 2020
- 1st May 2020
- Academic Press
- Hardcover ISBN:
Dr. David Caballero is a senior Research Associate at the 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics at University of Minho (Portugal). Dr. Caballero has broad experience in the fields of biophysics, bioengineering, microfluidics, and nanotechnology. His main research interests include the development of novel bioengineered in vitro models to study the mechanistic determinants of cancer cell invasion. Currently, Dr. Caballero work focuses in the development of innovative multi-organ-on-chip tumor models using engineered native-like 3D biomaterials for drug screening applications.
Senior Research Associate, 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics, University of Minho, Portugal
Prof. Subhas C. Kundu is a European Research Area Chair and Professor at the 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho (Portugal). Earlier Prof. Kundu was a Professor in the Department of Biotechnology at the Indian Institute of Technology Kharagpur (India). He obtained his post-graduation and PhD in Genetics from Banaras Hindu University (India) and received his post-doctoral trainings at the Institute of Molecular Biology in Moscow, the York University in Canada, the Medical University in Lubeck (Germany), and the Brunel University in UK. His research interests include silk biomaterial matrices for biomedical applications including 3D cancer modelling for investigating tumor growth and progression. His present area of interest is the use of natural-based biomaterials for 3D cancer modelling and drug screening.
European Research Area Chair and Professor, 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Portugal
Prof. Rui L Reis is the Founder and the Director of the 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics, and Director of ICVS/3B´s Associate Laboratory, both at University of Minho (Portugal). He is also the CEO of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine and the Global Past-President of Tissue Engineering and Regenerative Medicine International Society (TERMIS). He is a recognized world expert in the TERM and biomaterials fields, and has edited several books, being an author/inventor of more than 1250 publications and around 75 patents. He co-founded several companies that raised important private investments. He has been awarded several major international prizes, including among others the Jean Leray and George Winter Awards (ESB), the Clemson Award (SFB) for contributions to the literature and both the TERMIS-EU award for contributions to the literature and the lifetime achievement award, as well as the UNESCO - International Life Sciences Award and the IET A. F. Harvey Engineering Research Prize. He was bestowed with 2 honoris causa degrees, and is a honorary Professor in major Universities in the UK, China and South Korea, as well as a member of the National Academy of Engineering (USA).
Director and Professor, 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Portugal
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