Cell-derived Matrices Part A

Cell-derived Matrices Part A

1st Edition - March 24, 2020

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  • Editors: David Caballero, Subhas Kundu, Rui Reis
  • Hardcover ISBN: 9780128201725
  • eBook ISBN: 9780128201732

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Cell-Derived Matrices, Part A, Volume 156, provides a detailed description and step-by-step methods surrounding the use of three-dimensional cell-derived matrices for tissue engineering applications. Biochemical, biophysical and cell biological approaches are presented, along with sample results. Specific chapters cover Anisotropic cell-derived matrices with controlled 3D architecture, Generation of functional fluorescently-labelled cell-derived matrices by means of genetically-modified fibroblasts, Bi-layered cell-derived matrices, Engineering clinically-relevant cell-derived matrices using primary fibroblasts, Decellularized matrices for bioprinting applications, and much more.

Key Features

  • Contains contributions from leading experts in the field from across the globe
  • Covers a wide array of topics on the use of cell-derived matrices for tissue engineering and regenerative medicine applications
  • Includes relevant, analysis-based topics, such as quantification of the mechanical properties, decellularization protocols, and innovative matrix engineering methods


Postgraduates and researchers in the area of tissue engineering, biophysics and cellular biology, and other related scientific areas

Table of Contents

  • Preface
    David Caballero, Subhas C Kundu and Rui L Reis

    Section 1: Decellularization and characterization methods

    1.A step-by-step protocol for generating human fibroblast cell-derived completely biological extracellular matrix scaffolds
    Dhavan Sharma, Morgan Ferguson and Feng Zhao

    2.Preparation of osteogenic matrices from cultured cells
    Carl A. Gregory, Eoin McNeill and Simin Pan

    3.Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches
    Fátima Raquel Maia, Rui Luís Reis and Joaquim Miguel Oiveira

    4.Characterization of the elastic properties of extracellular matrix models by atomic force microscopy
    J. Otero, D. Navajas and J. Alcaraz

    5.Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry
    João C. Silva, Marta S. Carvalho, Ke Xia, Joaquim M. S. Cabral, Claúdia L. da Silva, Frederico C. Ferreira, Deepak Vashishth and Robert J. Linhardt

    Section 2: Engineering cell-derived matrices with advanced characteristics

    6.Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices
    Janusz Franco-Barraza, Kristopher S. Raghavan, Tiffany Luong and Edna Cukierman

    7.Engineering cell-derived matrices with controlled 3D architectures for pathophysiological studies
    Enrico Almici, David Caballero, Joan Montero Boronat and Josep Samitier Martí

    8.Generation of fluorescent cell-derived-matrix to study 3D cell migration
    Amélie Luise Godeau, Hélène Delanoë-Ayari and Daniel Riveline

    9.Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM
    Insung Yong, Seung Won Oh and Pilnam Kim

    Section 3: Cell-derived matrices for biomedical applications

    10.Cell-derived matrices (CDM)—Methods, challenges and applications
    Cheng HW, Yuan MT, Li CW and Chan BP.

    11.Boosting collagen deposition with a lysyl oxidase/bone morphogenetic protein-1 cocktail
    Tamara Rosell-García and Fernando Rodríguez-Pascual

    12.Generating specific cell-derived matrices from human trabecular meshwork cell cultures for mechanistic studies
    Felix Yemanyi, Janice Vranka and VijayKrishna Raghunathan

    13.Methods in cell biology: Cell-derived matrices
    Elizabeth Pavez Loriè and Petra Boukamp

Product details

  • No. of pages: 350
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: March 24, 2020
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128201725
  • eBook ISBN: 9780128201732

About the Serial Volume Editors

David Caballero

Dr. David Caballero is a senior Research Associate at the 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics at University of Minho (Portugal). Dr. Caballero has broad experience in the fields of biophysics, bioengineering, microfluidics, and nanotechnology. His main research interests include the development of novel bioengineered in vitro models to study the mechanistic determinants of cancer cell invasion. Currently, Dr. Caballero work focuses in the development of innovative multi-organ-on-chip tumor models using engineered native-like 3D biomaterials for drug screening applications.

Affiliations and Expertise

Senior Research Associate, 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics, University of Minho, Portugal

Subhas Kundu

Subhas C. Kundu, PhD, is a Research Coordinator and former European Research Area Chair at the 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of the University of Minho, Portugal. He was a former Full Professor and founder Head of the Department of Biotechnology at the Indian Institute of Technology Kharagpur, India. His research interests include natural-based biomaterials matrices, particularly silk, for biomedical applications, including 3D in vitro cancer models for investigating tumour growth and progression.

Affiliations and Expertise

Research Coordinator and former European Research Area Chair, 3B´s Research Group, I3Bs, Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Portugal.

Rui Reis

Dr. Rui Reis is Vice-President for Research and Innovation of University of Minho, Portugal, Director of 3B’s Research Group and Director of ICVS/3B´s Associate Laboratory, both of UMinho. He’s the CEO of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, the Coordinator of the Discoveries Centre for Regenerative and Precision Medicine, the Global Past-President of the Tissue Engineering and Regenerative Medicine International Society and the Editor-in-chief of the Journal of Tissue Engineering and Regenerative Medicine. He’s edited 18 books, 10 special issue journals, 280 book chapters and has more than 1225 published works listed on ISI Web of Knowledge, being an inventor of around 70 patents. He’s been awarded many important international prizes, including the TERMIS-EU contributions to the literature Award and the TERMIS-EU Career Achievement Award, and recently the UNESCO- International Life Sciences Award and the IET A. F. Harvey Engineering Research Prize.

Affiliations and Expertise

President of Institute 3B’s, University of Minho; Director of the Associated Laboratory ICVS/3B’s and of the European Institute of Excellence in Tissue Engineering and Regenerative Medicine (EXPERTISSUES); Full Professor of Tissue Engineering, Regenerative Medicine and Stem Cells, University of Minho, Barco, Portugal

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  • David C. Tue Oct 15 2019

    David Caballero

    An outstanding series of chapters written by top leaders in the field describing a large variety of methods and applications of cell-derived matrices. Highly recommended!