Cell-derived Matrices Part A, Volume 156
1st Edition
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Table of Contents
Preface
David Caballero, Subhas C Kundu and Rui L Reis
Section 1: Decellularization and characterization methods
1.A step-by-step protocol for generating human fibroblast cell-derived completely biological extracellular matrix scaffolds
Dhavan Sharma, Morgan Ferguson and Feng Zhao
2.Preparation of osteogenic matrices from cultured cells
Carl A. Gregory, Eoin McNeill and Simin Pan
3.Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches
Fátima Raquel Maia, Rui Luís Reis and Joaquim Miguel Oiveira
4.Characterization of the elastic properties of extracellular matrix models by atomic force microscopy
J. Otero, D. Navajas and J. Alcaraz
5.Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry
João C. Silva, Marta S. Carvalho, Ke Xia, Joaquim M. S. Cabral, Claúdia L. da Silva, Frederico C. Ferreira, Deepak Vashishth and Robert J. Linhardt
Section 2: Engineering cell-derived matrices with advanced characteristics
6.Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices
Janusz Franco-Barraza, Kristopher S. Raghavan, Tiffany Luong and Edna Cukierman
7.Engineering cell-derived matrices with controlled 3D architectures for pathophysiological studies
Enrico Almici, David Caballero, Joan Montero Boronat and Josep Samitier Martí
8.Generation of fluorescent cell-derived-matrix to study 3D cell migration
Amélie Luise Godeau, Hélène Delanoë-Ayari and Daniel Riveline
9.Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM
Insung Yong, Seung Won Oh and Pilnam Kim
Section 3: Cell-derived matrices for biomedical applications
10.Cell-derived matrices (CDM)—Methods, challenges and applications
Cheng HW, Yuan MT, Li CW and Chan BP.
11.Boosting collagen deposition with a lysyl oxidase/bone morphogenetic protein-1 cocktail
Tamara Rosell-García and Fernando Rodríguez-Pascual
12.Generating specific cell-derived matrices from human trabecular meshwork cell cultures for mechanistic studies
Felix Yemanyi, Janice Vranka and VijayKrishna Raghunathan
13.Methods in cell biology: Cell-derived matrices
Elizabeth Pavez Loriè and Petra Boukamp
Description
Cell-Derived Matrices, Part A, Volume 156, provides a detailed description and step-by-step methods surrounding the use of three-dimensional cell-derived matrices for tissue engineering applications. Biochemical, biophysical and cell biological approaches are presented, along with sample results. Specific chapters cover Anisotropic cell-derived matrices with controlled 3D architecture, Generation of functional fluorescently-labelled cell-derived matrices by means of genetically-modified fibroblasts, Bi-layered cell-derived matrices, Engineering clinically-relevant cell-derived matrices using primary fibroblasts, Decellularized matrices for bioprinting applications, and much more.
Key Features
- Contains contributions from leading experts in the field from across the globe
- Covers a wide array of topics on the use of cell-derived matrices for tissue engineering and regenerative medicine applications
- Includes relevant, analysis-based topics, such as quantification of the mechanical properties, decellularization protocols, and innovative matrix engineering methods
Readership
Postgraduates and researchers in the area of tissue engineering, biophysics and cellular biology, and other related scientific areas
Details
- No. of pages:
- 350
- Language:
- English
- Copyright:
- © Academic Press 2020
- Published:
- 24th March 2020
- Imprint:
- Academic Press
- Hardcover ISBN:
- 9780128201725
- eBook ISBN:
- 9780128201732
Ratings and Reviews
About the Serial Volume Editors
David Caballero
Dr. David Caballero is a senior Research Associate at the 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics at University of Minho (Portugal). Dr. Caballero has broad experience in the fields of biophysics, bioengineering, microfluidics, and nanotechnology. His main research interests include the development of novel bioengineered in vitro models to study the mechanistic determinants of cancer cell invasion. Currently, Dr. Caballero work focuses in the development of innovative multi-organ-on-chip tumor models using engineered native-like 3D biomaterials for drug screening applications.
Affiliations and Expertise
Senior Research Associate, 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics, University of Minho, Portugal
Subhas Kundu
Prof. Subhas C. Kundu is a European Research Area Chair and Professor at the 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho (Portugal). Earlier Prof. Kundu was a Professor in the Department of Biotechnology at the Indian Institute of Technology Kharagpur (India). He obtained his post-graduation and PhD in Genetics from Banaras Hindu University (India) and received his post-doctoral trainings at the Institute of Molecular Biology in Moscow, the York University in Canada, the Medical University in Lubeck (Germany), and the Brunel University in UK. His research interests include silk biomaterial matrices for biomedical applications including 3D cancer modelling for investigating tumor growth and progression. His present area of interest is the use of natural-based biomaterials for 3D cancer modelling and drug screening.
Affiliations and Expertise
European Research Area Chair and Professor, 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Portugal
Rui Reis
Prof. Rui L Reis is the Founder and the Director of the 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics, and Director of ICVS/3B´s Associate Laboratory, both at University of Minho (Portugal). He is also the CEO of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine and the Global Past-President of Tissue Engineering and Regenerative Medicine International Society (TERMIS). He is a recognized world expert in the TERM and biomaterials fields, and has edited several books, being an author/inventor of more than 1250 publications and around 75 patents. He co-founded several companies that raised important private investments. He has been awarded several major international prizes, including among others the Jean Leray and George Winter Awards (ESB), the Clemson Award (SFB) for contributions to the literature and both the TERMIS-EU award for contributions to the literature and the lifetime achievement award, as well as the UNESCO - International Life Sciences Award and the IET A. F. Harvey Engineering Research Prize. He was bestowed with 2 honoris causa degrees, and is a honorary Professor in major Universities in the UK, China and South Korea, as well as a member of the National Academy of Engineering (USA).
Affiliations and Expertise
Director and Professor, 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, Portugal
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