Description

General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Cell cycle regulators in mammalian systems * Cell cycle control in yeast and fungal systems * Analysis of cell cycle regulators in oocyte, egg, and embryonic systems, as well as general methods

Readership

Biochemists, cell biologists, molecular biologists, microbiologists, and developmental biologists.

Table of Contents

General Methodologies: A.A. Russo, Purification and Reconstitution of Cyclin-Dependent Kinase 2 in Four States of Activity. H. Wilhelm, S.S.L. Andersen, and E. Karsenti, Purification of Recombinant Cyclin B1/Cdc2 Kinase from Xenopus Egg Extracts. K.A. Resing and N.G. Ahn, Protein Phosphorylation Analysis by Electrospray Ionization Mass Spectrometry. C. Norbury and S. Moreno, Cloning Cell Cycle Regulatory Genes by Transcomplementation in Yeast. P.D. Adams, P. Lopez, W.R. Sellers, and W.G. Kaelin, Jr., Fluorescence-Activated Cell Sorting of Transfected Cells. N.J.C. Lamb and A. Fernandez, Microinjection of Antibodies into Mammalian Cells. K.D. Lustig, P.T. Stukenberg, T.J. McGarry, R.W. King, V.L. Cryns, P.E. Mead, L.I. Zon, J. Yuan, and M.W. Kirschner, Small Pool Expression Screening: Identification of Genes Involved in Cell Cycle Control, Apoptosis, and Early Development. J. Pines, Localization of Cell Cycle Regulators by Immunofluorescence. L. Meijer and S.-H. Kim, Chemical Inhibitors of Cyclin-Dependent Kinases. D.E. Bassett, Jr., M. Boguski, and P. Hieter, Identifying Human Homologs of Cell Cycle Genes using dbEST and XREFdb. C. Bai and S.J. Elledge, Gene Identification Using the Yeast Two-Hybrid System. Cell Cycle Regulators in Mammalian Systems: S. Freundlieb, U. Baron, A.L. Bonin, M. Gossen, and H. Bujard, Use of Tetracycline-Controlled Gene Expression Systems to Study Mammalian Cell Cycle. R.J. Sheaff, Regulation of Mammalian Cyclin-Dependent Kinase 2. D.E. Phelps and Y. Xiong, Assay for Activity of Mammalian Cyclin D-Dependent Kinases CDK4 and CDK6. J.R. Nevins, J. DeGregori, L. Jakoi

Details

No. of pages:
678
Language:
English
Copyright:
© 1997
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883953
Print ISBN:
9780121821845

About the serial-volume-editor

William Dunphy

Affiliations and Expertise

Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, U.S.A.

Reviews

@from:Praise for the Volume @qu:"This book will be invaluable to those studying the cell cycle, especially to students gaining experience in the techniques common to this field. The chapters have been written by those who are active in the field and have practical knowledge of the techniques involved. I would highly recommend the addition of this book to library and bookstores." @source:--Ada A. Cole, Rush Medical College of Rush University, for DOODY'S PUBLISHING REVIEWS @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive