Cell Culture in Phytochemistry

General Preface

Preface to Volume 4

Contents of Previous Volumes

Part I Introduction


1 Cell Culture in Phytochemistry

I. History

II. Cytodifferentiation

III. Outlook

References

Part II Accumulation of Phytochemicals


2 Physiology of the Accumulation of Secondary Metabolites with Special Reference to Alkaloids

I. Introduction

II. Biosynthesis

III. Accumulation of Secondary Metabolites

IV. Conclusion

References


3 The Compartmentation of Secondary Metabolites in Plant Cell Cultures

I. Introduction

II. The Analytical Approach to Cell Compartmentation

III. Compartmentation as a Result of Membrane Transport and Accumulation Processes at Specific Sites

IV. Compartmentation Related to Growth and Cell Specialization

V. Conclusion

References


4 Regulation of Synthesis of Phenolics

I. Introduction

II. Phenolic Production in Cultured Tissues

III. Regulation of Phenolic Synthesis

IV. Conclusion

References


5 Cell Growth and Accumulation of Secondary Metabolites

I. Introduction

II. Factors Controlling Growth in Plant Cell Cultures

III. Production of Secondary Metabolites and Control of Cell Growth

IV. Relationship Between Growth and Accumulation of Secondary Metabolites

References

Part III Special Techniques


6 Cell Cloning and the Selection of High Yielding Strains

I. Introduction

II. Clones and Cloning

III. Heterogeneity in Cell Cultures

IV. Stability of Clones and Subclones

V. High Yielding Strains

References


7 Selection of Mutants which Accumulate Desirable Secondary Compounds

I. Introduction

II. Selection Systems

III. Conclusion

References


8 New Approaches to Genetic Manipulation of Plants

I. Introduction

II. Somatic Hybridization by Protoplast Fusion

III. Isolated Plant Chromosomes as Potential Vectors for Gene Transfer

IV. DNA Transformation

V. Conclusion

References


9 Elicitation: Methodology and Aspects of Application

I. Introduction

II. Terminology

III. Elicitors and Their Mode of Action

IV. Methodology of Elicitation

V. Factors of Elicitor-Induced Accumulation

VI. Elicitation of Protoplasts and Isolated Cells

VII. Elicitation and Cell Ultrastructure

VIII. Induction and Proteinase Inhibitors

IX. Biochemical and Molecular Genetic Aspects

X. Conclusion

Appendix: Elicitor-Induced Product Accumulation as Presented at the Sixth IAPTC Congress, Minneapolis, 1986

References


10 Techniques, Characteristics, Properties, and Commercial Potential of Immobilized Plant Cells

I. Introduction

II. Early Research on Immobilization Systems

III. Techniques of Cell Immobilization

IV. Characteristics and Properties of Immobilized Plant Cells which Make Them Suitable for the Production of Chemicals

V. Bioreactor Configurations for Use with Immobilized Cells

VI. Future Commercial Exploitation

References


11 Cryopreservation of Secondary Metabolite-Producing Plant Cell Cultures

I. Introduction

II. Cryopreservation Components

III. Prospects

References


12 Plant Regeneration

I. Introduction

II. General Methodology

III. Modes of Plant Regeneration

IV. Regeneration in Medicinal Plant Tissue Cultures

References


13 Two-Phase Culture

I. Introduction

II. Accumulation Phases

III. Discussion

References


14 Continuous Culture of Plant Cells

I. Introduction

II. Characteristics of Plant Cells

III. Various Techniques for Continuous Culture of Plant Cells

IV. Fermentor Design

V. Theory and Practice of Continuous Culture Principles

VI. Biomass Production

VII. Metabolite Production

VIII. Future Prospects

References


15 Use of Immunoassays in the Detection of Plant Cell Products

I. Introduction

II. Methodology of Radioimmunoassay

III. Use of Radioimmunoassay in the Measurement of Phytochemicals

IV. Methodology of Enzyme-Linked Immunosorbent Assays (ELISAs)

V. Use of ELISA in the Measurement of Phytochemicals

VI. Conclusions

References

Index