Cell Culture in Phytochemistry - 1st Edition - ISBN: 9780127150048, 9780323155656

Cell Culture in Phytochemistry

1st Edition

Editors: Indra Vasil
eBook ISBN: 9780323155656
Imprint: Academic Press
Published Date: 28th December 1987
Page Count: 330
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Cell Culture and Somatic Cell Genetics of Plants, Volume 4: Cell Culture in Phytochemistry reviews phytochemistry by employing plant cell cultures.

This book discusses the realization of industrial plant cell culture for the production of phytochemicals and molecular biological approach to understand the regulation of product synthesis. The topics covered include the accumulation of secondary metabolites, phenolic production in cultured tissues, and stability of clones and subclones. The somatic hybridization by protoplast fusion, various techniques for continuous culture of plant cells, and methodology of enzyme-linked immunosorbent assays (ELISA) are also deliberated.

This volume provides intensive information on all aspects of plant cell and tissue culture and is recommended to both experienced researchers and to those newly entering the field.

Table of Contents

General Preface

Preface to Volume 4

Contents of Previous Volumes

Part I Introduction

1 Cell Culture in Phytochemistry

I. History

II. Cytodifferentiation

III. Outlook


Part II Accumulation of Phytochemicals

2 Physiology of the Accumulation of Secondary Metabolites with Special Reference to Alkaloids

I. Introduction

II. Biosynthesis

III. Accumulation of Secondary Metabolites

IV. Conclusion


3 The Compartmentation of Secondary Metabolites in Plant Cell Cultures

I. Introduction

II. The Analytical Approach to Cell Compartmentation

III. Compartmentation as a Result of Membrane Transport and Accumulation Processes at Specific Sites

IV. Compartmentation Related to Growth and Cell Specialization

V. Conclusion


4 Regulation of Synthesis of Phenolics

I. Introduction

II. Phenolic Production in Cultured Tissues

III. Regulation of Phenolic Synthesis

IV. Conclusion


5 Cell Growth and Accumulation of Secondary Metabolites

I. Introduction

II. Factors Controlling Growth in Plant Cell Cultures

III. Production of Secondary Metabolites and Control of Cell Growth

IV. Relationship Between Growth and Accumulation of Secondary Metabolites


Part III Special Techniques

6 Cell Cloning and the Selection of High Yielding Strains

I. Introduction

II. Clones and Cloning

III. Heterogeneity in Cell Cultures

IV. Stability of Clones and Subclones

V. High Yielding Strains


7 Selection of Mutants which Accumulate Desirable Secondary Compounds

I. Introduction

II. Selection Systems

III. Conclusion


8 New Approaches to Genetic Manipulation of Plants

I. Introduction

II. Somatic Hybridization by Protoplast Fusion

III. Isolated Plant Chromosomes as Potential Vectors for Gene Transfer

IV. DNA Transformation

V. Conclusion


9 Elicitation: Methodology and Aspects of Application

I. Introduction

II. Terminology

III. Elicitors and Their Mode of Action

IV. Methodology of Elicitation

V. Factors of Elicitor-Induced Accumulation

VI. Elicitation of Protoplasts and Isolated Cells

VII. Elicitation and Cell Ultrastructure

VIII. Induction and Proteinase Inhibitors

IX. Biochemical and Molecular Genetic Aspects

X. Conclusion

Appendix: Elicitor-Induced Product Accumulation as Presented at the Sixth IAPTC Congress, Minneapolis, 1986


10 Techniques, Characteristics, Properties, and Commercial Potential of Immobilized Plant Cells

I. Introduction

II. Early Research on Immobilization Systems

III. Techniques of Cell Immobilization

IV. Characteristics and Properties of Immobilized Plant Cells which Make Them Suitable for the Production of Chemicals

V. Bioreactor Configurations for Use with Immobilized Cells

VI. Future Commercial Exploitation


11 Cryopreservation of Secondary Metabolite-Producing Plant Cell Cultures

I. Introduction

II. Cryopreservation Components

III. Prospects


12 Plant Regeneration

I. Introduction

II. General Methodology

III. Modes of Plant Regeneration

IV. Regeneration in Medicinal Plant Tissue Cultures


13 Two-Phase Culture

I. Introduction

II. Accumulation Phases

III. Discussion


14 Continuous Culture of Plant Cells

I. Introduction

II. Characteristics of Plant Cells

III. Various Techniques for Continuous Culture of Plant Cells

IV. Fermentor Design

V. Theory and Practice of Continuous Culture Principles

VI. Biomass Production

VII. Metabolite Production

VIII. Future Prospects


15 Use of Immunoassays in the Detection of Plant Cell Products

I. Introduction

II. Methodology of Radioimmunoassay

III. Use of Radioimmunoassay in the Measurement of Phytochemicals

IV. Methodology of Enzyme-Linked Immunosorbent Assays (ELISAs)

V. Use of ELISA in the Measurement of Phytochemicals

VI. Conclusions




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© Academic Press 1987
Academic Press
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About the Editor

Indra Vasil

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