Cell Culture and Its Application - 1st Edition - ISBN: 9780120430505, 9780323142618

Cell Culture and Its Application

1st Edition

Editors: Ronald Acton
eBook ISBN: 9780323142618
Imprint: Academic Press
Published Date: 28th January 1977
Page Count: 770
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Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology. The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research.
The book is organized into four symposium and workshop sessions, encompassing 45 chapters. The opening chapter recognizes the interlocking relationship of cell culture technology and substantive cell biology. Chapters 2-5 describe the biochemical events that mark the cell cycle, with emphasis on occurrence of histone phosphorylation at each cycle. A discussion on cell differentiation, as a phenomena of interacting, inductive, and inhomogeneous cell populations, is included in these chapters. The second symposium session deals with signs of a revolution in progress in cell culture technology. This includes impact of tissue culture in physiological research course and in understanding of integrated physiology.
The last two symposium sessions cover the large-scale production of virus from tissue cultures for cell antigens. An approach to the study of aging using diploid human cells in culture as a model system is also presented. It involves isolation and characterization of HLA antigens from cultured cells and their contribution to the study of disease. A brief discussion on mycoplasma contamination, microplasma-cell-virus interaction, and advantages and limitations of direct and indirect culture for primary isolation and detection of mycoplasma contamination is provided. The book then proceeds by discussing cell differentiation of specific cell or organ, such as testis, sensory cell, hepatocyte, embryonic muscle cell, and brain cortex. The concluding chapters cover nutritional requirements for cell growth, defined culture media for specific cell type, issues and problems related to large-scale cell production, and quality control.
Cell biologists and researchers will find this book invaluable.

Table of Contents

List of Contributors


Opening Remarks

Symposium I. Cell Culture for the Study of Differentiation and Cell Cycle

Sequential Biochemical Events in the Cell Cycle

Aggregate Cultures: A Model for Studies of Brain Development

Differentiation of T Cells In Vitro

Summary Remarks

Symposium II. In Vitro Stimulators of Growth

Fibroblast Growth Factor: Effect on Growth of Fibroblasts and Related Cell Types

Human Epidermal Growth Factor

Summary Remarks

Symposium III. Large-Scale Production

Problems Encountered in Large-Scale Cell Production Plants

Large-Scale Production of Murine Lymphoblastoid Cell Lines Expressing Differentiation Alloantigens

Computer Control of Mammalian Cell Suspension Cultures

Optimizing Parameters for Growth of Anchorage-Dependent Mammalian Cells in Microcarrier Culture

Summary Remarks

Symposium IV. Cell Culture for the Study of Disease

Regulation of Proliferative Capacity during Aging in Cell Culture

Isolation and Characterisation of HLA Antigens from Cultured Cells and Their Contribution to the Study of Disease

New Approaches to Human Lysosomal Storage Diseases Using Animal Cells and Animal Viruses

Summary Remarks

Plenary Sessions

Mycoplasma Contamination of Cell Cultures: A Status Report

Recent Studies—RNA Tumor Viruses

Workshop I. Differentiation

Cell Differentiation of Juvenile Chick Testis II. Conversion of Progesterone to Testosterone In Vitro

Differentiation of Sensory Cells in Cultures of Aggregates of Dissociated Embryonic Chick Otocysts

A Model for the "Permissive" Effect of Glucocorticoids on the Glucagon Induction of Amino Acid Transport in Cultured Hepatocytes

Cytoplasmic Messenger Ribonucleoproteins and Translational Control during Growth and Differentiation of Embryonic Muscle Cells

Explants of Newborn Rat Brain Cortex Show Mutual Influence of Fatty Acid Patterns after Combination at Different Ages in Organotypic Cultures

Use of In Vitro Cell Colony Assays for Measuring the Cytotoxicity of Chemotherapeutic Agents to Hematopoietic Progenitor Cells Committed to Myeloid, Erythroid and Megakaryocytoid Differentiation

Melanogenesis in Somatic Cell Hybrids

Morphological Transforming Factor: Influence on Histotypic Organization of Epithelial Brain Cells

Alterations in the Phenotypic Expression of Cultured Articular Chondrocytes by a Pituitary Growth Factor (CGF)

Restoration of Pigment Synthesis in Mutant Melanocytes after Fusion with Chick Embryo Erythrocytes

Workshop II. Nutritional Requirements for Cell Growth

The Serine and Glycine Requirements of Cultured Human Lymphocyte Lines

Autoclavable Low Cost Cell Culture Media

Qualitative and Quantitative Analysis of Nutritional Factors for Cell Growth

Saline Washes Stimulate the Proliferation of Human Fibroblasts in Culture

Workshop III. Problems of Large-Scale Cell Culture and Quality Control

Tissue Culture Reagent Quality Assurance Testing in a Large-Scale Virus Production Laboratory

Contamination Monitoring and Control in a Large-Scale Tissue Culture Virus Production Laboratory

Mass Cell Cultures in a Controlled Environment

Growth of BHK Cells for the Production and Purification of Foot-and-Mouth Disease Virus

Significance of Oxidation-Reduction Potential in Mammalian Cell Cultures

Human Cell Cultures as Sentinel Systems for Monitoring Air Quality

Workshop IV. Cell Culture For The Study of Disease

Fat Body Culture Systems in the Study of Frog Diseases

Microassay of Bluetongue Virus from Culicoides variipennis

Human Colorectal Cell Lines as a Source for Carcinoembryonic Antigen (CEA)

The Elaboration of Glycoproteins into Culture Medium by Human Ovarian Tumor Cells

Establishment and Characterization of Epithelial Cell Cultures from Tumors of the Human Urinary Tract

Cardiac Muscle Cell Culture as a Pharmacological Tool: The Effect of Coronary Dilators on the Metabolism of Adenosine


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© Academic Press 1977
Academic Press
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About the Editor

Ronald Acton

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