Cell Biology Assays - 1st Edition - ISBN: 9780123756923, 9780123756930

Cell Biology Assays

1st Edition


Paperback ISBN: 9780123756923
eBook ISBN: 9780123756930
Imprint: Academic Press
Published Date: 16th November 2009
Page Count: 394
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Protein assay methods are used for protein identification with blood groups, cell surface markers, drugs and toxins. This text features comprehensive protocols essential for researchers studying various areas of biological and medical sciences. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a vareity of technologies and model systems.

Key Features

  • Focus on protein identification using mass spectrometry
  • Step-by-step procedures detailing materials, procedures, comment and pitfalls
  • Information on the plethora of technologies needed to tackle complex problems


Graduate students and professionals in a lab enviornment

Table of Contents

  1. Protein Determination
  2. Phosphopeptide Mapping: A Basic Protocol
  3. Coupling of Fluorescent Tags to Proteins
  4. Radioiodination of Proteins and Peptides
  5. Free-Flow Electrophoresis
  6. Gel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis.
  7. High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome Analysis
  8. Two-Dimensional Difference Gel Electrophoresis: Application for the Analysis of Differential Protein Expression in Multiple Biological Samples
  9. Affinity Electrophoresis for Studies of Biospecific Interations: High-Resolution Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies into Monoclonal Antibodies in Murine Blood Plasma
  10. Image Analysis and Quantitation
  11. Fluorescence Detection of Proteins in Gels Using SYPRO Dyes
  12. Autoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity in Flat Samples
  13. Two-Dimensional Gel Profiling of Posttanslationally Modified Proteins by in vivo Isotope Labeling
  14. Immunoprecipitation of Proteins under Nondenaturing Conditions
  15. Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interations: Applications in the Analysis of Mitochrondrial Oxidative Phosphorylation Complexes
  16. Affinity purification with Natural Immobilized Ligands
  17. Analysis of Protein-Protein Interactions by Chemical Cross-Linking
  18. Peroxisomal Targeting as a Tool to Assess Protein-Protein Interactions
  19. Biomolecular Interaction Analysis Mass Spectrometry
  20. Blot Overlays with P-Labedled GST-Ras Fusion Proteins: Application to Mapping Protein-Protein Interaction Sites
  21. Ligand Blot Overlay Assay: Detetion of Ca+2- and Small GTP-Binding Proteins
  22. Modular Scale Yeast


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© Academic Press 2010
Academic Press
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