Description

This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems.

Key Features

  • Provides researchers with solutions in lab environments
  • Features an array of essential methods, including endocytic pathways, membranes, mitochondria, and in vitro motility
  • Information on a plethora of technologies needed to tackle complex problems

Readership

Graduate student and professionals in a lab enviornment

Table of Contents

  1. Permeabilized Epithelial Cells to Study Exocytic Membrane Transport
  2. Studying Excit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches
  3. Assays Measuring Membrane Transport in the Endocytic Pathway
  4. Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport In Mammalian Cells
  5. Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells
  6. Use of Permeabilsed Mast Cells to Analyze Regulated Exocytosis
  7. Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading
  8. Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers
  9. Protein Translocation into Mitochondria
  10. Polarographic Assays of Mitochrondrial Functions
  11. Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells
  12. Heterokaryons: An Assay for Nucleocytoplasmic Shuttling
  13. DNA Replication-Dependent Chromatin Assembly System
  14. Cygnets: Intracellular Guanosine 3’,5-Cycli Monophosphate Sensing in Primary Cells Using Fluorescence Energy Transfer
  15. Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)
  16. Ratiometric Pericam
  17. Fuorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells
  18. In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins
  19. Dissecting Pathways; in Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication
  20. Detection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent Proteins
  21. Tracking Individual Chromosomes with Integrated Arrays of Iacop Sites and GFP-laci Repressor: Analyzing Position and Dy

Details

No. of pages:
298
Language:
English
Copyright:
© 2010
Published:
Imprint:
Academic Press
Print ISBN:
9780123751522
Electronic ISBN:
9780123751539