Cell Biology A Comprehensive Treatise V3

Cell Biology A Comprehensive Treatise V3

Gene Expression: The Production of RNA's

1st Edition - May 28, 1980

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  • Editor: David M. Prescott
  • eBook ISBN: 9780323143257

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Cell Biology, A Comprehensive Treatise, Volume 3: Gene Expression: The Production of RNA’s mainly discusses the molecular and cytological bases of gene expression. The coverage begins with the concepts of organization of DNA and gene sequences in chromosomes, as an introduction to a more detailed coverage of gene expression. The book opens with a general discussion on the organization of DNA sequences in chromosomes. This chapter includes different methods of analyzing DNA sequences. As the book progresses, it looks upon the details on gene reiteration and amplification up to the transcription of prokaryotes and eukaryotes. It includes the ways of regulating transcription. The following chapters deal mostly with the structure and activity of genes up to the different virus strains in both RNA and DNA. The cytoplasmic and environmental impact on gene expression is also discussed. Chapter 8 generally tackles the DNA conformation and template function. The succeeding chapters focus on the transfer and ribosomal RNA as a result of maturation events; the processing of hnRNA and its relation to mRNA; and recombinant DNA procedures. The book closes with the directory of the different classes of cellular RNAs. This book will be helpful to many graduate students, teachers, scientists, and researchers in need of information regarding cell biology.

Table of Contents

  • List of Contributors


    Contents of Other Volumes

    1 The Organization of DNA Sequences in Chromosomes

    I. Introduction

    II. Methods of Analyzing DNA Sequences

    III. Characteristics of the Different Kinetic Classes of Sequences

    IV. Interspersion of Sequences of Different Kinds

    V. Arrangement of Specific Structural Genes

    VI. Single-Copy Structural Genes

    VII. Concluding Remarks


    2 Gene Reiteration and Gene Amplification

    I. Introduction

    II. Alternatives to Multiple Genes

    III. Multiple Genes Integrated in the Germ Line Genome

    IV. Fluctuations in Tandem Gene Number

    V. Predictable Changes in rDNA Redundancy in Somatic Cells

    VI. Predictable Changes in rDNA Redundancy That Are Transmissible in the Germ Line-rDNA Magnification

    VII. The Evolution of Tandem Repeated Genes

    VIII. Amplification of a Unique Germ Line Gene in Tetrahymena

    IX. Amplification in the Germ Cells of Multicellular Organisms

    X. Concluding Remarks on the Peculiarity of Ribosomal DNA


    3 Basic Enzymology of Transcription in Prokaryotes and Eukaryotes

    I. Introduction

    II. Eukaryotic RNA Polymerases

    III. Purification Characteristics and General Properties of RNA Polymerases

    IV. Function of Individual Subunits of Core RNA Polymerase

    V. Interrelationship of RNA Polymerases

    VI. Conclusions


    4 Regulation of Transcription in Prokaryotes, Their Plasmids, and Viruses

    I. The Scope of This Chapter

    II. The Basic Mechanics of the Transcription Process

    III. Different Ways of Regulating Transcription

    IV. How Different Combinations of Factors Are Put Together to Control Specific Genes

    V. Overall Patterns of Transcriptional Control Observed in Different Gene Systems

    VI. Concluding Remarks


    5 Structural Manifestation of Nonribosomal Gene Activity

    I. Introduction

    II. Chromatin in Nonpolytene Somatic Cells

    III. Polytene Chromosomes

    IV. Chromosomes during Meiosis

    V. Comparative Aspects


    6 The Expression of Animal Virus Genes

    I. Introduction

    II. The RNA-Containing Viruses

    III. The DNA-Containing Viruses

    IV. Remarks


    7 Aspects of Cytoplasmic and Environmental Influences on Gene Expression

    I. Introduction

    II. Cytoplasmic Influences on Gene Expression in Transplanted Nuclei

    III. Cytoplasmic Determinants in Embryogeny

    IV. Behavior of Nuclei in Somatic Cell Heterokaryons

    V. Steroid Hormone Influences on Target Cell Nuclei

    VI. "Gene Amplification" in Cultured Mammalian Cells

    VII. Conclusions


    8 Molecular Aspects of the Regulation of Eukaryotic Transcription: Nucleosomal Proteins and Their Postsynthetic Modifications in the Control of DNA Conformation and Template Function

    I. Introduction

    II. Control of DNA Conformation

    III. DNA-Associated Nonhistone Proteins

    IV. Conclusion


    9 Maturation Events Leading to Transfer RNA and Ribosomal RNA

    I. Introduction

    II. Approaches to the Study of RNA Processing

    III. Transfer RNA Biosynthesis

    IV. Ribosomal RNA Biosynthesis

    V. Degradation of RNA


    10 The Processing of hnRNA and Its Relation to mRNA

    I. Introduction

    II. Labeling of hnRNA

    III. The Sequence Complexities of Nuclear and Cytoplasmic RNA's

    IV. Precursors for Specific mRNA's

    V. Structural Gene Sequences and Introns

    VI. Histone Gene Transcription

    VII. Polyadenylation and Methylation

    VIII. Nuclear Ribonucleoprotein

    IX. Additional Genetic Aspects

    X. Conclusions


    11 Recombinant DNA Procedures in the Study of Eukaryotic Genes

    I. Introduction

    II. Methods for in Vitro Recombination

    III. Cloning Vectors

    IV. Methods for Screening Recombinant Clones for Specific DNA Sequences

    V. Isolation of Single-Copy Structural Gene Sequences

    VI. Isolation and Characterization of Specific Eukaryotic Genes

    VII. Identification of Regulatory Sequences in Cloned Eukaryotic DNA


    12 Basic Characteristics of Different Classes of Cellular RNA's: A Directory

    I. Introduction

    II. Nuclear RNA's

    III. Cytoplasmic RNA's

    IV. Mitochondrial RNA's



Product details

  • No. of pages: 698
  • Language: English
  • Copyright: © Academic Press 1980
  • Published: May 28, 1980
  • Imprint: Academic Press
  • eBook ISBN: 9780323143257

About the Editor

David M. Prescott

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