Cannabinoids and Their Receptors, Volume 593

1. Mouse Neuroblastoma CB1 Cannabinoid Receptor-Stimulated [35S]GTPɣS Binding: Total and Antibody-Targeted Gα Protein-Specific Scintillation Proximity Assays
   Khalil Eldeeb, Sandra Leone-Kabler and Allyn C. Howlett
2. Protocols and Good Operating Practices in the Study of Cannabinoid Receptors
   Linda M. Console-Bram, Pingwei Zhao and Mary E. Abood
3. Real-Time Measurement of Cannabinoid Receptor-Mediated cAMP Signaling
   Morag R. Hunter, David B. Finlay, Christa E. Macdonald, Erin E. Cawston, Natasha L. Grimsey  and Michelle Glass
4. Techniques for the Cellular and Subcellular Localization of Endocannabinoid Receptors and Enzymes in the Mammalian Brain
   Luigia Cristino, Roberta Imperatore and Vincenzo Di Marzo
5. Endocannabinoid Transport Proteins: Discovery of Tools to Study Sterol Carrier Protein-2
   Cecilia J. Hillard, Huan Huang, Caleb D. Vogt, Beatriz E. Rodrigues, Terrence S. Neumann, Daniel S. Sem, Friedhelm Schroeder and Christopher W. Cunningham
6. Lipidomics: A Corrective Lens for Enzyme Myopia
   Heather B. Bradshaw and Emma Leishman
7. Functional Analysis of Mitochondrial CB1 Cannabinoid Receptors (mtCB1) in the Brain
   Su Melser, Antonio C.P. Zottola, Roman Serrat, Nagore Puente, Pedro Grandes, Giovanni Marsicano and Etienne Hebert-Chatelain
8. Modeling Neurodegenerative Disorders for Developing Cannabinoid-Based Neuroprotective Therapies
   Javier Fernández-Ruiz, María Gómez-Ruiz, Concepción García, Mariluz Hernández and José A. Ramos
9. Metabolic Profiling of CB1 Neutral Antagonists
   Herbert H. Seltzman, Rangan Maitra, Katharine Bortoff, Jay Henson, Patricia H. Reggio, Daniel Wesley and Joseph Tam
10. Ligand-Assisted Protein Structure (LAPS): An Experimental Paradigm for Characterizing Cannabinoid-Receptor Ligand-Binding Domains
    David R. Janero, Anisha Korde and Alexandros Makriyannis
11. New Methods for the Synthesis of Cannabidiol Derivatives
    Ana Lago-Fernández, Vanessa Redondo, Laura Hernández-Folgado, Laura Figuerola-Asencio and Nadine Jagerovic
12. Approaches to Assess Biased Signaling at the CB1R Receptor
    Robert B. Laprairie, Edward L. Stahl and Laura M. Bohn
13. Design and Synthesis of Cannabinoid 1 Receptor (CB1R) Allosteric Modulators: Drug Discovery Applications
    Abhijit R. Kulkarni, Sumanta Garai, David R. Janero and Ganesh A. Thakur
14. Assessing Allosteric Modulation of CB1 at the Receptor and Cellular Levels
    Caitlin E. Scott and Debra A. Kendall
15. Purification of Functional CB1 and Analysis by Site-Directed Fluorescence Labeling Methods
    J.F. Fay and D.L. Farrens
16. Mass Spectrometry Analysis of Human CB2 Cannabinoid Receptor and Its Associated Proteins
    Zhao-Hui Song, Alyssa S. Laun and Jian Cai
17. Expression and NMR Structural Studies of Isotopically Labeled Cannabinoid Receptor Type II
    Alexei Yeliseev and Klaus Gawrisch
18. Methods for the Development of In Silico GPCR Models
    Paula Morales, Dow P. Hurst and Patricia H. Reggio
19. Molecular Dynamics Methodologies for Probing Cannabinoid Ligand/Receptor Interaction
    Diane L. Lynch, Dow P. Hurst, Derek Shore, Mike Pitman and Patricia H. Reggio
    

Cannabinoids and Their Receptors, Volume 593, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This updated volume includes comprehensive chapters on a variety of topics, including Real time cAMP signaling in response to CB1 activation, CB1 signaling in mitochondria, Lipidomics of cannabinoid systems, Studying endocannabinoid transport, Metabolic profiling of CB1 neutral antagonists, Approaches to assess biased signaling at the CB1 receptor, and the Development of CB1 allosteric modulators.

• Continues the legacy of this premier serial with a new and updated release
• Covers research cannabinoids and their receptors

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists