Preface. 1. Introduction. 2. Homotypic and Heterotypic Cell Adhesion in Metastasis. 2.1 Release of malignant cells from the tumor mass: intercellular cohesion. 2.2 Malignant tumor cells in the blood stream: interactions with blood. 2.3 Adhesion to the target organ. 3. Motility, Deformability and Metastasis. 3.1 Motility and metastasis. 3.2 The role of active and passive deformability in invasion and resistance to shear stress forces in the blood stream. 4. ECM Degradation and Invasion. 4.1 Degradation. 4.2 Invasion. 5. The Role of Growth Interactions in Cancer Metastasis. 5.1 Methods to evaluate growth interactions in vitro. 5.2 Growth interactions in vivo. 6. Selection of Metastatic Variants. 6.1 Selection of organ-specific metastatic variants. 6.2 Selection of metastatic variants with enhanced or decreased metastatic abilities. 7. Genetic Tagging as a Mean to Study Tumor Progression or Metastasis-related Genes. 7.1 Clonal dominance in tumor progression. 7.2 Visualization of cancer metastasis. 7.3 Genes controlling the metastatic phenotype: use of gene tags to identify metastasis-related genes. 8. In Vivo Cancer Metastasis Assays.
8.1 Why study metastasis in vivo? 8.2 What defines an appropriate model of metastasis? 8.3 Cell lines. 8.4 Considerations regarding animals. 8.5 Site of injection. 8.6 Materials needed. 8.7 Spontaneous metastasis assay. 8.8 Experimental metastasis assay. 8.9 Enumeration of metastases. 8.10 Statistical considerations. 8.11 The influence of stress. 8.12 Concluding remarks. 9. Angiogenesis and Metastasis. 9.1 The corneal assay for angiogenesis. 9.2 The chick embryo chorioallantoic membrane assay. 9.3 Subcutaneous implant assay. References.