Calculations for Molecular Biology and Biotechnology

2nd Edition

A Guide to Mathematics in the Laboratory

Authors: Frank Stephenson Frank Stephenson
Paperback ISBN: 9780123756909
eBook ISBN: 9780123756916
Imprint: Academic Press
Published Date: 28th June 2010
Page Count: 460
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Description

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing.

Key Features

Key Features:

  • Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology
  • Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation
  • Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text

New to this Edition:

  • Updated and increased coverage of real time PCR and the mathematics used to measure gene expression
  • More sample problems in every chapter for readers to practice concepts

Readership

Any Biology student with an interest in Biotechnology or Molecular Biology and new Biotechnology technicians.

Table of Contents

Chapter 1 Scientific Notation and Metric Prefixes Introduction 1.1 Significant Digits 1.1.1 Rounding Off Significant Digits in Calculations 1.2 Exponents and Scientific Notation 1.2.1 Expressing Numbers in Scientific Notation 1.2.2 Converting Numbers from Scientific Notation to Decimal Notation 1.2.3 Adding and Subtracting Numbers Written in Scientific Notation 1.2.4 Multiplying and Dividing Numbers Written in Scientific Notation 1.3 Metric Prefixes 1.3.1 Conversion Factors and Canceling Terms Chapter Summary Chapter 2 Solutions, Mixtures, and Media Introduction 2.1 Calculating Dilutions – A General Approach 2.2 Concentrations by a Factor of X 2.3 Preparing Percent Solutions 2.4 Diluting Percent Solutions 2.5 Moles and Molecular Weight – Definitions 2.5.1 Molarity 2.5.2 Preparing Molar Solutions in Water with Hydrated Compounds 2.5.3 Diluting Molar Solutions 2.5.4 Converting Molarity to Percent 2.5.5 Converting Percent to Molarity 2.6 Normality 2.7 pH 2.8 pKa and the Henderson – Hasselbalch Equation Chapter Summary Chapter 3 Cell Growth 3.1 The Bacterial Growth Curve 3.1.1 Sample Data 3.2 Manipulating Cell Concentration 3.3 Plotting OD550 vs. Time on a Linear Graph 3.4 Plotting the Logarithm of OD550 vs. Time on a Linear Graph 3.4.1 Logarithms 3.4.2 Sample OD550 Data Converted to Logarithm Values 3.4.3 Plotting Logarithm OD550 vs. Time 3.5 Plotting the Logarithm of Cell Concentration vs. Time 3.5.1 Determining Logarithm Values 3.6 Calculating Generation Time 3.6.1 Slope and the Growth Constant 3.6.2 Generation Time 3.7 Plotting Cell Growth Data on a Semilog Graph 3.7.1 Plotting OD550 vs. Time on a Semilog Graph 3.7.2 E

Details

No. of pages:
460
Language:
English
Copyright:
© Academic Press 2011
Published:
Imprint:
Academic Press
eBook ISBN:
9780123756916
Paperback ISBN:
9780123756909

About the Author

Frank Stephenson

Frank Stephenson received his doctorate in molecular biology from UC Berkeley and has published several books in the field including 'DNA: How the Biotech Revolution is Changing the Way We Fight Disease' and 'A Hands-On Introduction to Forensic Science: Cracking the Case'. He is currently an instructor in the Technical Training Department with ThermoFisher Scientific, the world’s leading manufacturer of instrumentation and reagents for the biotechnology industry.

Affiliations and Expertise

Technical Training Department, ThermoFisher Scientific, San Diego, CA, USA

Frank Stephenson

Frank Stephenson received his doctorate in molecular biology from UC Berkeley and has published several books in the field including 'DNA: How the Biotech Revolution is Changing the Way We Fight Disease' and 'A Hands-On Introduction to Forensic Science: Cracking the Case'. He is currently an instructor in the Technical Training Department with ThermoFisher Scientific, the world’s leading manufacturer of instrumentation and reagents for the biotechnology industry.

Affiliations and Expertise

Technical Training Department, ThermoFisher Scientific, San Diego, CA, USA