Table of Contents

Chapter 1 Scientific Notation and Metric Prefixes Introduction 1.1 Significant Digits 1.1.1 Rounding Off Significant Digits in Calculations 1.2 Exponents and Scientific Notation 1.2.1 Expressing Numbers in Scientific Notation 1.2.2 Converting Numbers from Scientific Notation to Decimal Notation 1.2.3 Adding and Subtracting Numbers Written in Scientific Notation 1.2.4 Multiplying and Dividing Numbers Written in Scientific Notation 1.3 Metric Prefixes 1.3.1 Conversion Factors and Canceling Terms Chapter Summary Chapter 2 Solutions, Mixtures, and Media Introduction 2.1 Calculating Dilutions – A General Approach 2.2 Concentrations by a Factor of X 2.3 Preparing Percent Solutions 2.4 Diluting Percent Solutions 2.5 Moles and Molecular Weight – Definitions 2.5.1 Molarity 2.5.2 Preparing Molar Solutions in Water with Hydrated Compounds 2.5.3 Diluting Molar Solutions 2.5.4 Converting Molarity to Percent 2.5.5 Converting Percent to Molarity 2.6 Normality 2.7 pH 2.8 pKa and the Henderson – Hasselbalch Equation Chapter Summary Chapter 3 Cell Growth 3.1 The Bacterial Growth Curve 3.1.1 Sample Data 3.2 Manipulating Cell Concentration 3.3 Plotting OD550 vs. Time on a Linear Graph 3.4 Plotting the Logarithm of OD550 vs. Time on a Linear Graph 3.4.1 Logarithms 3.4.2 Sample OD550 Data Converted to Logarithm Values 3.4.3 Plotting Logarithm OD550 vs. Time 3.5 Plotting the Logarithm of Cell Concentration vs. Time 3.5.1 Determining Logarithm Values 3.6 Calculating Generation Time 3.6.1 Slope and the Growth Constant 3.6.2 Generation Time 3.7 Plotting Cell Growth Data on a Semilog Graph 3.7.1 Plotting OD550 vs. Time on a Semilog Graph 3.7.2 E

Details

No. of pages:
460
Language:
English
Copyright:
© 2011
Published:
Imprint:
Academic Press
Print ISBN:
9780123756909
Electronic ISBN:
9780123756916

About the authors

Frank Stephenson

Frank Stephenson received his doctorate in molecular biology from UC Berkeley and has published several books in the field including 'DNA: How the Biotech Revolution is Changing the Way We Fight Disease' and 'A Hands-On Introduction to Forensic Science: Cracking the Case'. He is currently an instructor in the Technical Training Department with ThermoFisher Scientific, the world’s leading manufacturer of instrumentation and reagents for the biotechnology industry.

Affiliations and Expertise

Technical Training Department, ThermoFisher Scientific, San Diego, CA, USA

Frank Stephenson

Frank Stephenson received his doctorate in molecular biology from UC Berkeley and has published several books in the field including 'DNA: How the Biotech Revolution is Changing the Way We Fight Disease' and 'A Hands-On Introduction to Forensic Science: Cracking the Case'. He is currently an instructor in the Technical Training Department with ThermoFisher Scientific, the world’s leading manufacturer of instrumentation and reagents for the biotechnology industry.

Affiliations and Expertise

Technical Training Department, ThermoFisher Scientific, San Diego, CA, USA