Calcium in Living Cells

Calcium in Living Cells

2nd Edition - November 11, 2010
  • Editor: Michael Whitaker
  • Hardcover ISBN: 9780123748416
  • eBook ISBN: 9780080951225

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Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases.

Key Features

  • Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research
  • Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field
  • Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research
  • Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging


Researchers in cell, molecular and developmental biology, biochemists

Table of Contents

      1. A Practical Guide to the Preparation of Ca2+ Buffers
      2. Donald M. Bers, Chris W. Patton and Richard Nuccitelli

      3. Photorelease Techniques for Raising or Lowering Intracellular Ca2+

    Robert Zucker

    3. Making and Using Calcium-Selective Mini- and Microelectrodes

    L. Hove-Madsen, S. Baudet and D.M. Bers

    4. Construction, Theory and Practical Considerations for using Self-Referencing of Ca2+-Selective Microelectrodes for Monitoring Extracellular Ca2+ Gradients

    Mark A. Messerli and Peter J.S. Smith

    5. Practical Aspects of Measuring Intracellular Calcium Signals with Fluorescent Indicators

    Joseph P. Y. Kao, Gong Li, Darryl A. Auston

    6. Genetically-encoded probes for measurement of intracellular calcium

    Michael Whitaker

    7. Patch clamp methods for studying calcium channels

    David L. Armstrong, Christian Erxleben and Jody A. White

    8. Nuclear Patch-Clamp Recording from Inositol 1,4,5-Trisphosphate Receptors

    Taufiq Rahman, Colin W. Taylor

    9. Confocal And Multiphoton Imaging Of Intracellular Ca2+

    Godfrey Smith, Martyn Reynolds, Francis Burto1, Ole Johan Kemi

    10. The Use of Aequorins to Record and Visualize Ca2+ Dynamics: From Subcellular Microdomains to Whole Organisms.

    Sarah E. Webb, Kelly L. Rogers, Eric Karplus, and Andrew L. Miller

Product details

  • No. of pages: 328
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: November 11, 2010
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123748416
  • eBook ISBN: 9780080951225

About the Serial Volume Editor

Michael Whitaker

Affiliations and Expertise

Department of Physiological Sciences Medical School, Newcastle upon Tyne, England