An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.

Key Features

*Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
* Chapters written by leaders in the field
* Tried and true methods deliver busy researchers a one-stop compendium of essential protocols


Researchers and students in cell, molecular and developmental biology

Table of Contents




PART I Genetics and Functional Genomics

Chapter 1: Mapping Mutations in C. elegans

I. Introduction

II. Mutant Origination

III. Mutations That Can Be Propagated as Homozygotes

IV. Mutations That Cannot Be Propagated as Homozygotes

V. Modifier Mutations

VI. SNP Mapping

VII. WGS and Beyond

Chapter 2: Specialized Chromosomes and Their Uses in Caenorhabditis elegans

I. Introduction

II. Chromosomal Rearrangements

III. Characterization of Chromosomal Rearrangements

IV. Engineered Constructs: Transgenic Arrays

Chapter 3: Genome Engineering by Transgene-Instructed Gene Conversion in C. elegans

I. Introduction

II. General Features of the MosTIC Technique and Experimental Outline

III. Methods

IV. Materials

V. Discussion: MosTIC Applications and Derivatives

VI. Summary

Chapter 4: RNAi Methods and Screening: RNAi Based High-Throughput Genetic Interaction Screening

I. Introduction: Large-Scale RNAi Screening in C. elegans

II. Methods

III. Materials

PART II Molecular Biology and Biochemistry

Chapter 5: Dissection of Genetic Pathways in C. elegans

I. Introduction

II. Strategies for Identifying Components in Pathways of Interest

III. Ordering Genes into Pathways

IV. Future Outlook

Chapter 6: Transgenesis in C. elegans

I. Introduction

II. Uses for Transgenes in C. elegans

III. Construction of Transgenes

IV. Obtaining Transgenic Animals

V. Perspectives: What Lies on the Horizon?

VI. Summary

Chapter 7: RNA Processing in C. elegans

I. Introduction

II. RNA Processing in Other Organisms

III. RNA Processing in C. elegans

Chapter 8: Analysis of microRNA Expression and Function

I. Introducti


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Academic Press
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