Caenorhabditis elegans: Cell Biology and Physiology

Caenorhabditis elegans: Cell Biology and Physiology

2nd Edition - January 16, 2012

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  • Editors: Joel Rothman, Andrew Singson
  • eBook ISBN: 9780123948267
  • Hardcover ISBN: 9780123946201

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Description

The second part of an updated edition of the classic Methods in Cell Biology, Volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.

Key Features

  • Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
  • Chapters written by leaders in the field
  • Tried and true methods deliver busy researchers a one-stop compendium of essential protocols

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

  • High-Resolution Imaging of Cellular Processes in Caenorhabditis elegans

    I. Microscope Stand

    II. Objective Lenses and Resolution

    III. Transmitted Light Imaging

    IV. Fluorescence Imaging

    V. Detectors

    VI. Confocal Imaging

    VII. Deconvolution Imaging

    VIII. Whole Worm (Adult and Larval Stages)

    IX. Embryos

    Immunofluorescence Microscopy

    I. Introduction

    II. Preparing Specimens for Immunolocalization Studies

    III. Preparation of Antibodies to Nematode Antigens

    IV. Microscopy: Necessary Equipment and Imaging Tips

    V. Concluding Remarks

    VI. Recipes

    Fluorescent Protein Methods: Strategies and Applications

    I. Introduction

    II. Methods

    III. Adopting New Fluorescent Proteins for Use in C. elegans

    Modern Electron Microscopy Methods for C. elegans

    I. Introduction

    II. Modern Electron Microscopes

    III. Alternate Immersion Fixation Techniques

    IV. Serial Thin Sectioning

    V. ImmunoEM Protocols

    VI. Alternate Methods to Post Stain Thin Sections

    VII. Electron Tomography

    VIII. Emerging EM Technologies

    IX. Computer-Based Analytical Tools

    X. Landmarks for Recognizing Tissues

    Acknowledgments

    Culture and Manipulation of Embryonic Cells

    I. Introduction: Uses and Limitations of the System

    II. Embryo Devitellinization and Blastomere Isolation

    III. Culture Methods

    IV. Drug Treatments

    V. Radioactive Labeling

    VI. Fixation, Cytochemistry, and Electron Microscopy

    VII. Solutions and Culture Media

    Laser Microsurgery in Caenorhabditis elegans

    I. Overview

    II. Identifying Cells in C. elegans

    III. Laser Ablation Theory and Apparatus

    IV. Laser Killing of Cells

    V. Assessing Damage to the Operated Cell

    VI. Unintended Damage

    VII. Laser Cutting of Nerve Fibers

    VIII. Related Methods

    Acknowledgments

    Methods in Cell Biology: Analysis of Cell Polarity in C. elegans Embryos

    I. Introduction

    II. Methods for Analyzing Gene Function

    III. Analyzing Cell Polarity: Protein and Organelle Localization

    IV. Dynamics of Polarity Establishment

    V. Summary

    Analysis of Membrane-Bound Organelles

    I. Introduction

    II. Visualizing Organelles

    III. Functional Studies

    IV. Protein Extract and Organelle Isolation

    V. Summary

    Acknowledgments

    C. elegans Cell Cycle Analysis

    I. Introduction

    II. Types of C. elegans cell cycles

    III. Using the C. elegans Lineage to Follow Cell Divisions

    IV. Methods of Developmental Synchronization

    V. Quantification of DNA Content

    VI. Detection of Different Cell Cycle Phases

    VII. Common Cell Cycle Phenotypes and Potential Interpretations

    Methods for Studying Programmed Cell Death in C. elegans

    I. Introduction

    II. Methods

    III. Conclusions

    Acknowledgments

    Methods for Studying the DNA Damage Response in the Caenorhabdatis elegans Germ Line

    I. Introduction

    II. Assay Development

    III. Assays

    IV. Materials

    V. Discussion

    Acknowledgements

    Analysis of Aging in Caenorhabditis elegans

    I. Introduction

    II. Aging Pathways and Their Mechanism of Action

    III. Life-Span Analysis

    IV. Analysis and Interpretation of Lifespan Data

    V. Measurement of Age-Related Changes

    VI. Conclusion

    Acknowledgments

    Analyses of C. elegans Fat Metabolic Pathways

    I. Introduction

    II. Staining of Fixed Animals and Histochemical Methods

    III. Biochemical Methods

    IV. Biochemical Measurements of Metabolic Rates

    V. Visualization of Fat Depots and Fat Metabolism Using Vital Dyes

    VI. Visualizing Hypodermal, Intestinal, and Gonad Lipid Stores using Nile Red

    VII. Fatty Acid Uptake

    VIII. Raman Scattering-Based Methods of Lipid Imaging in C. elegans

    IX. Summary

    Acknowledgments

    Electrophysiological Methods for Caenorhabditis elegans Neurobiology

    I. Introduction

    II. Recording Currents from Identified Neurons

    III. Recording Synaptic Events from Identified Neurons

    IV. Recording Currents and Synaptic Events at the Neuromuscular Junction

    V. Materials

    VI. Discussion

    VII. Summary

    Acknowledgments

Product details

  • No. of pages: 512
  • Language: English
  • Copyright: © Academic Press 2012
  • Published: January 16, 2012
  • Imprint: Academic Press
  • eBook ISBN: 9780123948267
  • Hardcover ISBN: 9780123946201

About the Serial Volume Editors

Joel Rothman

Andrew Singson

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