Analytical and Synthetic Methods:
G. Livesey and P. Lund, Determination of Branched-Chain Amino and Keto Acids with Leucine Dehydrogenase.
S.J. Yeaman, K.G. Cook, A.P. Bradford, and S.M.A. Jones, Analysis of Phosphorylation Sites on Branched-Chain 2-Ketoacid Dehydrogenase Complex.
D.J. Aberhart and J.-A. Cotting, High-Performance Liquid Chromatographic Separation of (3R) and (3S)-/-Leucine Using Marfey's Reagent.
D.J. Aberhart, High-Performance Liquid Chromatographic Separation of ~ga- and /-Leucine.
A.L. Gasking, W.T.E. Edwards, A.H. Frohock, M. Elia, and G. Livesey, Quantitative High-Performance Liquid Chromatographic Analysis of Branched-Chain 2-Keto Acids in Biological Samples.
O.A. Mamer and J.A. Montgomery, Determination of Branched-Chain 2-Hydroxy and 2-Keto Acids by Mass Spectrometry.
P.L. Crowell, R.H. Miller and A.E. Harper, Measurement of Plasma and Tissue Levels of Branched-Chain ~ga-Keto Acids by Gas-Liquid Chromatography.
J.A. Montgomery and O.A. Mamer, Determination of Methylmalonic Acid in Biological Fluids by Mass Spectrometry.
B.E. Corkey, Analysis of Acyl Coenzyme A Esters in Biological Samples.
M.T. King, P.D. Reiss, and N.W. Cornell, Determination of Short-Chain Coenzyme A Compounds by Reversed-Phase High-Performance Liquid Chromatography.
K. Bartlett and A.G. Causey, Radiochemical High-Performance Liquid Chromatography Methods for the Study of Branched-Chain Amino Acid Metabolism.
J.V. Schloss, R. Magolda, and M. Emptage, Synthesis of ~ga-lsopropylmalate, /-Isopropylmalate, and Dimethylcitraconate.
R.A. LaRossa and T.K. Van Dyk, Utilization of Sulfometuron Methyl, an Acetolactate Synthase Inhibitor, in Molecular Biological and Metabolic Studies of
This volume includes chapters on the newest techniques for measuring metabolites of branched-chain amino acids, many new enzyme assays such as the measurement of isozymes of acetohydroxacid synthase, and the purification of recently described enzymes such as isovaleryl-CoA dehydrogenase.
Biochemists, microbiologists, analytical chemists, geneticists, biomedical researchers, plant biochemists.
- No. of pages:
- © Academic Press 1988
- 28th November 1988
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY
University of Texas, Austin, U.S.A.
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA