Brain Receptor Methodologies Pt A
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Brain Receptor Methodologies Pt A

General Methods and Concepts. Amines and Acetylcholine

1st Edition - January 28, 1984

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  • Editor: Paul J. Marangos
  • eBook ISBN: 9780323152808

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Description

Brain Receptor Methodologies, Part A, General Methods and Concepts: Amines and Acetylcholine provides information pertinent to neurotransmitter and neuromodulator receptors in brain. This book explores the methodologies that can used to address several basic and clinical problems. Organized into two sections encompassing 18 chapters, this book starts with an overview of the receptor concept, which can be validated from indirect evidence obtained in studies of the quantitative aspects of drug antagonism. This text then examines the radioligand–receptor binding interactions. Other chapters consider immunocytochemistry, which has a primary role in determining the precise distribution of regulatory peptides to neural and endocrine elements of the diffuse neuroendocrine system. The final chapter discusses the use of the radioligand binding procedure for the study of muscarinic receptors, which has expanded the area of muscarinic receptor pharmacology. Biochemists, pharmacologists, physiologists, and researchers engaged in the fields of neurobiology and neuroscience will find this book extremely useful.

Table of Contents


  • Contributors

    General Preface

    Preface to Part A

    Contents of Part B

    Section I General Methods and Concepts Relating to Receptor Studies

    A. Some Introductory Considerations Relevant to Receptor Studies

    1. Receptors: A Historical Perspective

    2 . Preparation of Labeled Receptor Ligands

    3 . Heterogeneous Receptors and Binding Curve Analysis in Neurobiology

    4. The Distribution of Peripheral Regulatory Peptides: A Dual Immunochemical (Immunocytochemistry and Radioimmunoassay) Approach

    B. Methods for Studying the Receptor Molecule

    5. The Solubilization of Membrane Proteins

    6. Solubilization and Characterization of Brain Benzodiazepine Binding Sites

    7. Solubilization of the Dopamine Receptor

    8. Autoradiographic Demonstration of Receptor Distributions

    9. Affinity and Photoaffinity Labeling of Receptors

    C. Methods for Studying Receptor-Linked Effector Mechanisms

    10. Cyclic Nucleotide and Adenylate Cyclase in Brain: Electrophysiological Studies

    11. Calmodulin in the Nervous System

    12. Preparation of the Catalytic Subunit of cAMP-Dependent Protein Kinase

    13. Phospholipid Methylation in Brain and Other Tissues

    Section II Specific Receptor-Binding Methodologies

    A. Amines and Acetylcholines

    14. ß-Adrenergic Receptors

    15. α-Adrenergic Receptors in Neural Tissues: Methods and Applications of Radioligand Binding Assays

    16. Dopamine Receptors in Brain

    17. Radioactive Ligand Binding Studies: Identification of Central Serotonin Receptors

    18. Muscarinic Receptor [3H]Ligand Binding Methods

    Index

Product details

  • No. of pages: 384
  • Language: English
  • Copyright: © Academic Press 1984
  • Published: January 28, 1984
  • Imprint: Academic Press
  • eBook ISBN: 9780323152808

About the Editor

Paul J. Marangos

Dr. Marangos is author of 252 peer-reviewed research papers on neurobiology, and 4 books – three by Academic Press (the Neurobiological Research series of volumes) and a fourth titled “Emerging Strategies in Neuroprotection”. He is a founding editor of “The Journal of Molecular Neuroscience” and inventor on 14 issued patents. He spent 13 years at the NIMH and was adjunct professor of neurochemistry at GW School of Medicine during that time. He is co-Founder of five Biotech firms with over 20 years of experience as CEO of both public and private life science companies. He has had extensive experience in all aspects of the pharmaceutical and biotechnology industries from bench to product. This coupled with his academic experience has provided a global perspective on the entire medical R&D enterprise, in particular its faults, and remedies to speed the quest for cures.

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